Factsheet for PGSC0003DMT400024816

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 4
extracellular 2
endoplasmic reticulum 2
vacuole 2
plasma membrane 2
golgi 2
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytoskeleton, cytosol, secretory
BaCelLo:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	cytosol
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	cytoskeleton
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400018924
PGSC0003DMT400065786
PGSC0003DMT400074537

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G53180.1	PGSC0003DMT400065786	AT1G14340.1	21798944
AT3G53180.1	PGSC0003DMT400074537	AT2G31955.1	21798944
AT3G53180.1	PGSC0003DMT400018924	AT4G25660.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
PGSC0003DMT400048927	Potato	cytosol	34.06	71.24
Zm00001d033947_P001	Maize	cytosol	15.0	42.86
PGSC0003DMT400072665	Potato	cytosol	44.06	39.94
Zm00001d047936_P001	Maize	cytosol	20.62	36.87
Zm00001d044569_P001	Maize	cytosol	20.62	36.87
Zm00001d031055_P001	Maize	cytosol	17.5	34.78
Zm00001d021367_P001	Maize	cytosol	18.12	28.02
PGSC0003DMT400072398	Potato	cytosol	40.94	27.93
GSMUA_AchrUn_...	Banana	cytosol	42.5	26.31
PGSC0003DMT400048929	Potato	cytosol, plastid	14.06	26.16
PGSC0003DMT400072666	Potato	cytosol	52.81	19.95
VIT_08s0007g04670.t01	Wine grape	cytosol	45.94	17.5
KRH32570	Soybean	cytosol, nucleus	44.38	16.99
TraesCS1A01G143000.1	Wheat	cytosol	43.44	16.53
Bra028548.1-P	Field mustard	cytosol	42.19	16.52
Zm00001d032815_P011	Maize	cytosol	43.44	16.51
TraesCS1D01G141800.1	Wheat	golgi	43.44	16.51
EER91525	Sorghum	cytosol	43.44	16.51
HORVU1Hr1G038060.9	Barley	cytosol	43.44	16.47
TraesCS1B01G158600.1	Wheat	cytosol	43.12	16.39
CDY13313	Canola	cytosol	41.88	16.38
CDX73575	Canola	cytosol	41.88	16.34
AT3G53180.1	Thale cress	cytosol	43.44	16.31
Bra006975.1-P	Field mustard	cytosol	43.12	16.29
CDX78151	Canola	cytosol	43.12	16.29
CDX67559	Canola	cytosol	41.56	15.99
Os10t0456500-02	Rice	plasma membrane	42.19	15.98
TraesCS6D01G065600.1	Wheat	cytosol	40.31	15.83
HORVU6Hr1G012290.5	Barley	cytosol	40.0	15.18
GSMUA_AchrUn_...	Banana	cytosol	1.88	1.88
PGSC0003DMT400048935	Potato	cytosol	1.56	1.86
PGSC0003DMT400024814	Potato	cytosol	1.56	1.66
KRH32567	Soybean	endoplasmic reticulum	2.19	1.62
KRH75546	Soybean	plastid	0.31	0.43

Protein Annotations

KEGG:00220+6.3.1.2	KEGG:00250+6.3.1.2	KEGG:00630+6.3.1.2	KEGG:00910+6.3.1.2	Gene3D:3.30.590.10	MapMan:35.2
GO:GO:0003674	GO:GO:0003824	GO:GO:0004356	GO:GO:0006807	GO:GO:0008150	GO:GO:0008152
InterPro:Gln_synth/guanido_kin_cat_dom	InterPro:Gln_synth_cat_dom	UniProt:M1AKN4	PFAM:PF00120	EnsemblPlantsGene:PGSC0003DMG403009595	PGSC:PGSC0003DMG403009595
EnsemblPlants:PGSC0003DMT400024816	PANTHER:PTHR43785	PANTHER:PTHR43785:SF2	SMART:SM01230	SUPFAM:SSF55931	UniParc:UPI000296868A

Description

Glutamate-ammonia ligase [Source:PGSC_GENE;Acc:PGSC0003DMG403009595]

Coordinates

chr1:-:27407755..27416055

Molecular Weight (calculated)

35766.7 Da

IEP (calculated)

5.909

GRAVY (calculated)

-0.159

Length

320 amino acids

Sequence
(BLAST)

001: MCPDVQHFHC LILYGDKYMD LNVIYVTIVR FFYQVSGDGK ETWARIDKTS YCSTTAIDDA SLVLQEIVAS LHSFNILVEQ VKAGKGQFEI VLGYTDCGRA
101: ANNLIITHEV VKGVARKHGL LASFTPRYYN NNVCPQSYAH STDDDVGSGS HVHISLFKNG ENIFMASGEP NSYERLIPKA RNSSFICWGI ECKELPVRAC
201: CPPGVPNGVV TNFEMKTFDG SANPHLGLAS IIIAGIDGMR RKLPIPGPVE PESYDFDWNN DDIKLPWSLR CAIIHMDSDK WFNEMLGKEF ICTRGGVVGE
301: EIGHDFCGGD EKYKHIMFNY

Best Arabidopsis Sequence Match ( AT3G53180.1 )
(BLAST)

001: MEFSELKEAI EKIELVDAHA HNIVSLDSSF PFIGTFSEAA GDALTFAPHS LSFKRNLREI AQLYGTEVSL EAVEEHRKTS GLDSFTSKCF KEARISALLI
101: DDGLKLDKKH DIEWHRNFVP FVGRVLRIET LAEQILEEEC PGGYFYGSES TEPPVWDLDS FTKTFVERLN SLVPEIVALK TIAAYRSGLD IDTYVSKEVA
201: ENGLVEVLRA GKPVRIGNKG LIDYILTISL EVAVRRDLPL QIHTGFGDKD LDLRLSNPLH LRTLLEDKRF GKCRIVLLHA AYPFSKEASF LSSVYPQVYL
301: DFGLAVPKLS VHGMVSSVKE LLDLASIKKV MFSTDGYASP ETYYLGAKKA REVIFLVLSD ACASGDLSLM EAIDAAKDIF SRNSIGFYKL NIDTDSSSPQ
401: NIISPKLKIK EPDVQEDSSS FVRIIWVDTS GQQRCRAVQA QRFNRSVKKN GVGLTFASMG MTSFTDGPAE ESKLTGVGEI RLVPDLSTKQ TIPWTKQESM
501: VLADMQLKPG EAWGYCPRET LRRVAKVLKD EFDLVMNAGF ENEFYLLKNV VREGKEEYMP FDFGPYCATS SFDAASPIFH DIVPALESLN IEVEQFHAES
601: GKGQFEVSLG HTIASHAADN LVYTREVIRS VARKQGLLAT FVPKYDYCDI GSGSHVHLSL WKNGENVFPA SNNSSSHGIS SVGEEFMAGV LFHLPSILAI
701: IAPLPNSYDR IQPNTWSGAF QCWGKENREA ALRAASPPGT PDGLVTNFEI KSFDGSANPH LGLAVIMAAG IDGLRRHLQL PTPIDINPAD VAATLNRLPE
801: TLSEAVEALD KDKVLHDLLG QKLLVAIKGV RKAEVEYYSK NPDAYKQLIH RY

Arabidopsis Description

Nodulin/glutamine synthase-like protein [Source:UniProtKB/TrEMBL;Acc:F4J9A0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.