CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

nucleus 1
cytosol 3
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, nucleus
Any Predictor:	cytosol, mitochondrion, nucleus, plastid
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	plastid
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH08874
KRH13856
KRH35244
KRH38387
KRH43566
KRH69305

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G53180.1	KRH69305	AT1G14340.1	21798944
AT3G53180.1	KRH13856	AT2G31955.1	21798944
AT3G53180.1	KRH43566	AT2G31955.1	21798944
AT3G53180.1	KRH08874	AT4G25660.1	21798944
AT3G53180.1	KRH35244	AT4G25660.1	21798944
AT3G53180.1	KRH38387	AT4G25660.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH75546	Soybean	plastid	20.69	75.22
VIT_08s0007g04670.t01	Wine grape	cytosol	71.53	71.19
PGSC0003DMT400072665	Potato	cytosol	28.95	68.56
Solyc10g083830.1.1	Tomato	extracellular	49.64	67.7
AT3G53180.1	Thale cress	cytosol	67.58	66.31
CDX73575	Canola	cytosol	64.95	66.22
Bra006975.1-P	Field mustard	cytosol	66.99	66.12
CDX78151	Canola	cytosol	66.99	66.12
CDY13313	Canola	cytosol	63.4	64.79
CDX67559	Canola	cytosol	64.11	64.42
Bra028548.1-P	Field mustard	cytosol	62.92	64.38
KRH32567	Soybean	endoplasmic reticulum	33.01	64.04
GSMUA_AchrUn_...	Banana	cytosol	24.16	63.12
Zm00001d032815_P011	Maize	cytosol	62.56	62.11
Zm00001d031055_P001	Maize	cytosol	11.96	62.11
EER91525	Sorghum	cytosol	62.2	61.76
TraesCS1B01G158600.1	Wheat	cytosol	62.2	61.76
TraesCS1A01G143000.1	Wheat	cytosol	62.08	61.71
TraesCS1D01G141800.1	Wheat	golgi	61.96	61.52
GSMUA_AchrUn_...	Banana	cytosol	38.04	61.51
HORVU1Hr1G038060.9	Barley	cytosol	61.6	61.02
Zm00001d047936_P001	Maize	cytosol	13.04	60.89
Zm00001d044569_P001	Maize	cytosol	13.04	60.89
Os10t0456500-02	Rice	plasma membrane	61.24	60.59
Zm00001d033947_P001	Maize	cytosol	7.89	58.93
PGSC0003DMT400024814	Potato	cytosol	21.05	58.47
TraesCS6D01G065600.1	Wheat	cytosol	55.98	57.42
HORVU6Hr1G012290.5	Barley	cytosol	57.54	57.06
Solyc10g083550.1.1	Tomato	cytosol	26.08	54.64
Solyc06g011330.1.1	Tomato	cytosol	19.38	54.36
PGSC0003DMT400072666	Potato	cytosol	54.55	53.84
Solyc10g083850.1.1	Tomato	cytosol	52.39	52.58
PGSC0003DMT400048935	Potato	cytosol	16.75	52.04
Solyc01g056430.1.1	Tomato	cytosol	20.1	50.91
PGSC0003DMT400072398	Potato	cytosol	28.35	50.53
Solyc10g083840.1.1	Tomato	cytosol	9.69	50.31
Zm00001d021367_P001	Maize	cytosol	12.2	49.28
Solyc06g011290.1.1	Tomato	cytosol	21.65	46.41
PGSC0003DMT400024816	Potato	cytosol	16.99	44.38
Solyc01g056450.1.1	Tomato	cytosol	12.8	43.85
PGSC0003DMT400048929	Potato	cytosol, plastid	8.49	41.28
PGSC0003DMT400048927	Potato	cytosol	6.7	36.6

Protein Annotations

KEGG:00220+6.3.1.2	KEGG:00250+6.3.1.2	KEGG:00630+6.3.1.2	KEGG:00910+6.3.1.2	EntrezGene:100809101	Gene3D:3.10.20.70
Gene3D:3.20.20.140	Gene3D:3.30.590.10	MapMan:35.2	EMBL:ACUP02006129	InterPro:Amidohydro-rel	EnsemblPlantsGene:GLYMA_10G060200
GO:GO:0003674	GO:GO:0003824	GO:GO:0004356	GO:GO:0006542	GO:GO:0006807	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016787	InterPro:Gln_synt_N	InterPro:Gln_synth/guanido_kin_cat_dom
InterPro:Gln_synth_cat_dom	UniProt:I1L922	InterPro:IPR036651	EnsemblPlants:KRH32570	ProteinID:KRH32570	ProteinID:KRH32570.1
InterPro:Metal_Hydrolase	PFAM:PF00120	PFAM:PF04909	PANTHER:PTHR43785	PANTHER:PTHR43785:SF2	MetaCyc:PWY-381
MetaCyc:PWY-5675	MetaCyc:PWY-6549	MetaCyc:PWY-6963	MetaCyc:PWY-6964	SMART:SM01230	SUPFAM:SSF51556
SUPFAM:SSF54368	SUPFAM:SSF55931	UniParc:UPI0002965A7B	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr10:-:5585676..5601516

Molecular Weight (calculated)

92815.1 Da

IEP (calculated)

6.453

GRAVY (calculated)

-0.153

Length

836 amino acids

Sequence
(BLAST)

001: MELSELRKAV EEVELVDAHA HNIVSLDSNF AFIHAFSEAY GDAVTFSPHT LSFKRNLREI AELYGSELSL QGVEEHRRVS GMQSICSTCF KAARISAILI
101: DDGLQLDKKH DIEWHRSFTP LVGRILRIER LAEEILDEDL PDGSSWTVDS FTKAFVSKLK SVSGEIFGLK SIAAYRSGLE INTNVTKKDA EEGLRQVLIA
201: GKPVRIANKN LIDYIFLQSL EVAQSYDLPM QIHTGFGDKD LDMRLSNPLH LRAVLEDKRY LKSRIVLLHA SYPFSREASY LASVYSQVYL DFGLAIPKLS
301: VHGMISSMKE LLELAPINKV MFSTDGYAFP ETFYLGAKKS REVVFSVLRD ACIDGDLSIP EAVEVAKDIF ARNAIHFYKI SSAIGVVSSH SNLPQKLNDG
401: LDIDLSLVRI LWVDGSGQHR CRAVPKKRFN DIVAKNGVGL AFATLGFSSY MDGPADGSGL TAVGETRLMP DLSTLTRIPW NKQDEMVLAD MCVKPGEAWE
501: YCPRDALRRA SKILKDEFDL EMNAGFENEF ILLKSITREG KEEWIPFDSS PYCSSSAFDA ASPILHEVAA SLHSLGISVE QLHAEAGKGQ FELVLKYTVC
601: TKAADNLTFT REVVRAIARK HGLLATFIPK YALDDLGSGS HVHLSLSRNG QNVYMASDRS SKHGISTLGK EFMAGILHHL PSILAFIAPL PNSYDRLQPN
701: TWSGAYLFWG NENKEAPLRA TSPPGTPDGL VSNFEMKSFD GSANPYLGLA AILAAGIDGL RRKLSLPEPV DTNPNPETLQ RLPASLSESL DALNKDDFLK
801: EFMSDKLLTT IKAIRKAEID HYTKHKDAYK QLIHRY

Best Arabidopsis Sequence Match ( AT3G53180.1 )
(BLAST)

001: MEFSELKEAI EKIELVDAHA HNIVSLDSSF PFIGTFSEAA GDALTFAPHS LSFKRNLREI AQLYGTEVSL EAVEEHRKTS GLDSFTSKCF KEARISALLI
101: DDGLKLDKKH DIEWHRNFVP FVGRVLRIET LAEQILEEEC PGGYFYGSES TEPPVWDLDS FTKTFVERLN SLVPEIVALK TIAAYRSGLD IDTYVSKEVA
201: ENGLVEVLRA GKPVRIGNKG LIDYILTISL EVAVRRDLPL QIHTGFGDKD LDLRLSNPLH LRTLLEDKRF GKCRIVLLHA AYPFSKEASF LSSVYPQVYL
301: DFGLAVPKLS VHGMVSSVKE LLDLASIKKV MFSTDGYASP ETYYLGAKKA REVIFLVLSD ACASGDLSLM EAIDAAKDIF SRNSIGFYKL NIDTDSSSPQ
401: NIISPKLKIK EPDVQEDSSS FVRIIWVDTS GQQRCRAVQA QRFNRSVKKN GVGLTFASMG MTSFTDGPAE ESKLTGVGEI RLVPDLSTKQ TIPWTKQESM
501: VLADMQLKPG EAWGYCPRET LRRVAKVLKD EFDLVMNAGF ENEFYLLKNV VREGKEEYMP FDFGPYCATS SFDAASPIFH DIVPALESLN IEVEQFHAES
601: GKGQFEVSLG HTIASHAADN LVYTREVIRS VARKQGLLAT FVPKYDYCDI GSGSHVHLSL WKNGENVFPA SNNSSSHGIS SVGEEFMAGV LFHLPSILAI
701: IAPLPNSYDR IQPNTWSGAF QCWGKENREA ALRAASPPGT PDGLVTNFEI KSFDGSANPH LGLAVIMAAG IDGLRRHLQL PTPIDINPAD VAATLNRLPE
801: TLSEAVEALD KDKVLHDLLG QKLLVAIKGV RKAEVEYYSK NPDAYKQLIH RY

Arabidopsis Description

Nodulin/glutamine synthase-like protein [Source:UniProtKB/TrEMBL;Acc:F4J9A0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.