Factsheet for PGSC0003DMT400072398

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 4
plastid 1
mitochondrion 1
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400018924
PGSC0003DMT400065786
PGSC0003DMT400074537

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G53180.1	PGSC0003DMT400065786	AT1G14340.1	21798944
AT3G53180.1	PGSC0003DMT400074537	AT2G31955.1	21798944
AT3G53180.1	PGSC0003DMT400018924	AT4G25660.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc10g083550.1.1	Tomato	cytosol	77.19	90.73
PGSC0003DMT400072665	Potato	cytosol	48.83	64.87
Zm00001d031055_P001	Maize	cytosol	17.48	50.93
Zm00001d047936_P001	Maize	cytosol	18.76	49.16
Zm00001d044569_P001	Maize	cytosol	18.76	49.16
Zm00001d033947_P001	Maize	cytosol	11.09	46.43
GSMUA_AchrUn_...	Banana	cytosol	50.11	45.45
PGSC0003DMT400048929	Potato	cytosol, plastid	15.35	41.86
PGSC0003DMT400024816	Potato	cytosol	27.93	40.94
Zm00001d021367_P001	Maize	cytosol	17.91	40.58
PGSC0003DMT400048927	Potato	cytosol	12.37	37.91
VIT_08s0007g04670.t01	Wine grape	cytosol	54.16	30.24
Bra028548.1-P	Field mustard	cytosol	49.68	28.52
CDY13313	Canola	cytosol	49.47	28.36
KRH32570	Soybean	cytosol, nucleus	50.53	28.35
CDX73575	Canola	cytosol	49.25	28.17
Bra006975.1-P	Field mustard	cytosol	50.11	27.75
CDX78151	Canola	cytosol	50.11	27.75
CDX67559	Canola	cytosol	49.04	27.64
AT3G53180.1	Thale cress	cytosol	49.25	27.11
HORVU1Hr1G038060.9	Barley	cytosol	47.76	26.54
TraesCS1A01G143000.1	Wheat	cytosol	47.55	26.52
Os10t0456500-02	Rice	plasma membrane	47.76	26.51
TraesCS1D01G141800.1	Wheat	golgi	47.55	26.48
TraesCS1B01G158600.1	Wheat	cytosol	47.33	26.37
PGSC0003DMT400072666	Potato	cytosol	47.33	26.21
Zm00001d032815_P011	Maize	cytosol	46.91	26.13
EER91525	Sorghum	cytosol	46.48	25.89
TraesCS6D01G065600.1	Wheat	cytosol	43.92	25.28
HORVU6Hr1G012290.5	Barley	cytosol	44.78	24.91
GSMUA_AchrUn_...	Banana	cytosol	1.49	2.19
PGSC0003DMT400048935	Potato	cytosol	1.07	1.86
PGSC0003DMT400024814	Potato	cytosol	1.07	1.66
KRH32567	Soybean	endoplasmic reticulum	1.07	1.16
KRH75546	Soybean	plastid	0.0	0.0

Protein Annotations

KEGG:00220+6.3.1.2	KEGG:00250+6.3.1.2	KEGG:00630+6.3.1.2	KEGG:00910+6.3.1.2	EntrezGene:107061364	Gene3D:3.10.20.70
Gene3D:3.30.590.10	MapMan:35.2	GO:GO:0003674	GO:GO:0003824	GO:GO:0004356	GO:GO:0006542
GO:GO:0006807	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	InterPro:Gln_synt_N
InterPro:Gln_synth/guanido_kin_cat_dom	InterPro:Gln_synth_cat_dom	InterPro:IPR036651	UniProt:M1CQ84	PFAM:PF00120	EnsemblPlantsGene:PGSC0003DMG400028171
PGSC:PGSC0003DMG400028171	EnsemblPlants:PGSC0003DMT400072398	PANTHER:PTHR43785	PANTHER:PTHR43785:SF2	SMART:SM01230	SUPFAM:SSF55931
UniParc:UPI0002959C65	RefSeq:XP_015165995.1	:	:	:	:

Description

Glutamate-ammonia ligase [Source:PGSC_GENE;Acc:PGSC0003DMG400028171]

Coordinates

chr10:-:55645299..55649317

Molecular Weight (calculated)

52175.2 Da

IEP (calculated)

4.878

GRAVY (calculated)

-0.200

Length

469 amino acids

Sequence
(BLAST)

001: MERCESSSVG EEGNYNSSDE PFKPVPFVRI LWIDNSGIHR CRVIPRERLS FVKKHGLGLS PACLAALSSV SNSPAEGTNL GFTGMIRIIP DLSTRCKIPW
101: EKQQEMTLAD MCIEPDKPWE YCPREVFRRV TKILKDEFDL VVNVGFEIEF YILKSVIKNG KEEWLPIDKT SYCSTSGFDV SSSILEDIVT YLQTMNITVE
201: QVHAETGKGQ FEVVLGYAEA STAIASLIYA REVIKSVARQ HGQMATFVPK YAEDEDGSGS HVHISLSRNG ENIFMASGDS KYGMSKIGES FMAGVLNHLP
301: AILAFTATHP LSYEHLVPKT RNAAYLCWGK ENTEAPLRTA SPPGIADDLV SHFEIKAFDA CTNPYIGLAS IIISGVDGLR KDLSLPKPVE GDSDVSGENL
401: RSVPDSISES LIALGEDTLF SEVMSENLLT AIKGIRKMEV KNYCEPERDY NFYDQQIYKD PLKDIIFKF

Best Arabidopsis Sequence Match ( AT3G53180.1 )
(BLAST)

001: MEFSELKEAI EKIELVDAHA HNIVSLDSSF PFIGTFSEAA GDALTFAPHS LSFKRNLREI AQLYGTEVSL EAVEEHRKTS GLDSFTSKCF KEARISALLI
101: DDGLKLDKKH DIEWHRNFVP FVGRVLRIET LAEQILEEEC PGGYFYGSES TEPPVWDLDS FTKTFVERLN SLVPEIVALK TIAAYRSGLD IDTYVSKEVA
201: ENGLVEVLRA GKPVRIGNKG LIDYILTISL EVAVRRDLPL QIHTGFGDKD LDLRLSNPLH LRTLLEDKRF GKCRIVLLHA AYPFSKEASF LSSVYPQVYL
301: DFGLAVPKLS VHGMVSSVKE LLDLASIKKV MFSTDGYASP ETYYLGAKKA REVIFLVLSD ACASGDLSLM EAIDAAKDIF SRNSIGFYKL NIDTDSSSPQ
401: NIISPKLKIK EPDVQEDSSS FVRIIWVDTS GQQRCRAVQA QRFNRSVKKN GVGLTFASMG MTSFTDGPAE ESKLTGVGEI RLVPDLSTKQ TIPWTKQESM
501: VLADMQLKPG EAWGYCPRET LRRVAKVLKD EFDLVMNAGF ENEFYLLKNV VREGKEEYMP FDFGPYCATS SFDAASPIFH DIVPALESLN IEVEQFHAES
601: GKGQFEVSLG HTIASHAADN LVYTREVIRS VARKQGLLAT FVPKYDYCDI GSGSHVHLSL WKNGENVFPA SNNSSSHGIS SVGEEFMAGV LFHLPSILAI
701: IAPLPNSYDR IQPNTWSGAF QCWGKENREA ALRAASPPGT PDGLVTNFEI KSFDGSANPH LGLAVIMAAG IDGLRRHLQL PTPIDINPAD VAATLNRLPE
801: TLSEAVEALD KDKVLHDLLG QKLLVAIKGV RKAEVEYYSK NPDAYKQLIH RY

Arabidopsis Description

Nodulin/glutamine synthase-like protein [Source:UniProtKB/TrEMBL;Acc:F4J9A0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.