CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

plastid 8
extracellular 2
endoplasmic reticulum 2
vacuole 2
plasma membrane 2
golgi 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	mitochondrion, plastid, secretory
BaCelLo:	plastid
ChloroP:	plastid
EpiLoc:	plastid
iPSORT:	secretory
MultiLoc:	plastid
Plant-mPloc:	plastid
Predotar:	mitochondrion
PProwler:	plastid
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	plastid

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH17939	Soybean	nucleus	97.37	96.89
PGSC0003DMT400022467	Potato	cytosol	84.98	88.76
KRH06666	Soybean	nucleus	83.85	87.81
KRG94871	Soybean	cytosol	83.85	87.81
HORVU4Hr1G002270.3	Barley	cytosol	82.85	86.42
KXG37902	Sorghum	cytosol	82.73	86.41
Zm00001d033480_P002	Maize	extracellular	82.6	86.27
GSMUA_Achr4P22700_001	Banana	cytosol	72.34	86.27
GSMUA_Achr7P01530_001	Banana	cytosol	82.6	86.16
KRH57885	Soybean	cytosol	82.48	86.14
KRH04756	Soybean	cytosol, endoplasmic reticulum, nucleus	82.48	86.14
Os12t0623900-01	Rice	extracellular	82.48	86.03
GSMUA_Achr5P02740_001	Banana	cytosol	72.09	85.97
TraesCS4A01G298700.1	Wheat	cytosol	82.35	85.9
Os12t0624000-02	Rice	extracellular	82.1	85.64
KXG24060	Sorghum	cytosol	81.98	85.51
Zm00001d031128_P004	Maize	plasma membrane, plastid	81.85	85.38
VIT_06s0009g01080.t01	Wine grape	plastid	85.36	84.83
KRH04805	Soybean	plastid	44.31	84.49
TraesCS5D01G031300.2	Wheat	golgi, mitochondrion, nucleus, plasma membrane, unclear	81.1	84.16
TraesCS5B01G022800.4	Wheat	extracellular, golgi, plastid, unclear	80.85	83.68
Solyc01g009180.2.1	Tomato	plastid	85.23	83.35
Zm00001d013644_P001	Maize	extracellular	82.48	82.69
KRH63013	Soybean	cytosol	11.64	81.58
GSMUA_Achr4P21470_001	Banana	mitochondrion	82.85	81.43
HORVU5Hr1G006780.7	Barley	cytosol	47.56	81.37
TraesCS5A01G024900.2	Wheat	cytosol	81.6	80.79
TraesCS4B01G014700.2	Wheat	vacuole	82.98	80.27
TraesCS4D01G012900.1	Wheat	mitochondrion	82.73	80.02
KRH04683	Soybean	mitochondrion	27.91	79.93
AT5G20980.2	Thale cress	mitochondrion	77.97	76.72
CDX70955	Canola	mitochondrion	78.22	76.69
Bra006589.1-P	Field mustard	mitochondrion, plastid	78.1	76.56
CDX88830	Canola	mitochondrion	78.1	76.56
KRH04804	Soybean	endoplasmic reticulum	34.04	75.77
CDX92419	Canola	plastid	77.35	70.55
CDY16584	Canola	plastid	77.1	65.19
KRH04746	Soybean	plastid	12.77	58.62

Protein Annotations

KEGG:00270+2.1.1.14	KEGG:00450+2.1.1.14	EntrezGene:100776541	Gene3D:3.20.20.210	MapMan:4.1.2.2.6.2.1	EMBL:ACUP02012319
InterPro:Cbl-indep_Met_Synth_N	InterPro:Cobalamin-indep_Met_synthase	EnsemblPlantsGene:GLYMA_20G055900	GO:GO:0003674	GO:GO:0003824	GO:GO:0003871
GO:GO:0005488	GO:GO:0005575	GO:GO:0005576	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005829	GO:GO:0008150	GO:GO:0008152	GO:GO:0008270	GO:GO:0008652	GO:GO:0008705
GO:GO:0009058	GO:GO:0009086	GO:GO:0009987	GO:GO:0016740	GO:GO:0032259	GO:GO:0050667
UniProt:I1JWK3	InterPro:IPR038071	EnsemblPlants:KRG89918	ProteinID:KRG89918	ProteinID:KRG89918.1	HAMAP:MF_00172
InterPro:Met_Synth_C/arc	PFAM:PF01717	PFAM:PF08267	PIRSF:PIRSF000382	PANTHER:PTHR30519	PANTHER:PTHR30519:SF11
MetaCyc:PWY-5041	MetaCyc:PWY-6151	MetaCyc:PWY-6936	MetaCyc:PWY-702	SUPFAM:SSF51726	TIGRFAMs:TIGR01371
UniParc:UPI000233A206	InterPro:UROD/MetE-like_sf	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr20:-:13720721..13726049

Molecular Weight (calculated)

88669.1 Da

IEP (calculated)

6.841

GRAVY (calculated)

-0.105

Length

799 amino acids

Sequence
(BLAST)

001: MYKLSSIQCV APFTISLCTL SFSSSSLRFS VRATSSRAMA SHIVGYPRMG PKRELKFALE SFWDGKSSAD DLQKVAADLR SAIWKQMADA GIKYIPSNTF
101: SYYDQVLDTT AMLGAVPSRY NWNGGEIGFD VYFSMARGNS SVPAMEMTKW FDTNYHYIVP ELGPDVKFSY ASHKVVDEYK EAKVLGINTV PVLVGPVSYL
201: LLSKPAKGVE KSFSLLSLID KILPVYREVV AELKAAGATW IQFDEPTLVK DLNAHQLQAF THAYAELESS LSGLNVLIET YFADVPAEAY KTLTSLKAVT
301: AYGFDIVRGT KTLDLIKQGF PSGKFLFAGV VDGRNIWANN FASSLNTLQA LGDIVGNDKV VVSTSCSLLH TAVDLVNETK LDQEIKSWLA FAAQKVVEVN
401: ALAKALSGQK DEVFFSANAA ALASRKSSPR VINEAVQKAA AALKGSDHRR ATNVSARLDA QQKKLNLSVL PTTTIGSFPQ TADLRRVRRE FKANKISEED
501: YIRFIKEEIY NVVKLQEELD IDVLVHGEPE RNDMVEYFGE QLSGFAFTAN GWVQSYGSRC VKPPIIYGDV SRPKPMTVFW SSTAQSLTKR PMKGMLTGPV
601: TILNWSFVRD DQPRFETCYQ IALAIKDEVE DLEKAGITVI QIDEAALREG LPLRKSEEAF YLNWAVHSFR ITNCGVEDTT QIHTHMCYSN FNDIIHSIID
701: MDADVITIEN SRSDEKLLSV FREGVKYGAG IGPGVYDIHS PRIPPTEEIA DRINKMLAVL ESSILWVNPD CGLKTRKYTE VKPALTNMVA AAKLIRNQL

Best Arabidopsis Sequence Match ( AT3G03780.1 )
(BLAST)

001: MASHIVGYPR MGPKRELKFA LESFWDGKSS ADDLQKVSAD LRSDIWKQMS AAGIKYIPSN TFSHYDQVLD TTAMLGAVPS RYGFTSGEIG LDVYFSMARG
101: NASVPAMEMT KWFDTNYHYI VPELGPEVKF SYASHKAVNE YKEAKALGVE TVPVLVGPVS YLLLSKLAKG VDKSFDLLSL LPKILPVYKE VIAELKAAGA
201: SWIQLDEPLF VMDLEGHKLQ AFSGAYAELE STLSGLNVLV ETYFADIPAE AYKTLTSLKG VTAFGFDLVR GTKTIDLIKS GFPQGKYLFA GVVDGRNIWA
301: NDLAASLITL QSLEGVVGKD KLVVSTSCSL LHTAVDLINE TKLDAEIKSW LAFAAQKVVE VDALAKALAG QTNESFFTAN ADALSSRRSS PRVTNESVQK
401: AAAALKGSDH RRTTEVSARL DAQQKKLNLP ILPTTTIGSF PQTVELRRVR REYKAKKISE EDYVKAIKEE IKKVVDIQED LDIDVLVHGE PERNDMVEYF
501: GEQLSGFAFT ANGWVQSYGS RCVKPPVIYG DVSRPKPMTV FWSSTAQSMT KRPMKGMLTG PVTILNWSFV RNDQPRHETC YQIALAIKDE VEDLEKGGIG
601: VIQIDEAALR EGLPLRKAEH SFYLDWAVHS FRITNCGVQD STQIHTHMCY SNFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
701: HSPRIPSTDE IADRINKMLA VLEQNILWVN PDCGLKTRKY TEVKPALKAM VDAAKLIRSQ LGSAK

Arabidopsis Description

MS25-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9SRV5]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.