Factsheet for Zm00001d033480_P002

Subcellular Localization

min:

: max

Winner_takes_all: extracellular

Predictor Summary:

cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	extracellular
Any Predictor:	cytosol, mitochondrion
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

extracellular: 20408568
extracellular: 22192489
plastid: 23198870
plasma membrane: 23508561
mitochondrion: 27297264

msms PMID: 27297264 doi

D Dahal, KJ Newton, BP Mooney

Division of Biological Sciences, ‡Department of Biochemistry, and §The Charles W Gehrke Proteomics Center, University of Missouri , Columbia, Missouri 65211, United States.

msms PMID: 22192489 doi

K Witzel, M Shahzad, A Matros, HP Mock, KH Mühling

Institute of Plant Nutrition and Soil Science, Christian Albrechts University, Hermann-Rodewald-Strasse 2, 24118 Kiel, Germany. khmuehling@plantnutrition.uni-kiel.de.

msms PMID: 23198870 doi

M Huang, G Friso, K Nishimura, X Qu, PD Olinares, W Majeran, Q Sun, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, United States.

msms PMID: 20408568 doi

W Ma, N Muthreich, C Liao, M Franz-Wachtel, W Schütz, F Zhang, F Hochholdinger, C Li

Department of Plant Nutrition, China Agricultural University, Beijing, PR China.

msms PMID: 23508561 doi

Z Zhang, P Voothuluru, M Yamaguchi, RE Sharp, SC Peck

Division of Biochemistry, University of Missouri Columbia, MO, USA ; Christopher S. Bond Life Sciences Center, University of Missouri Columbia, MO, USA ; Interdisciplinary Plant Group, University of Missouri Columbia, MO, USA.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KXG37902	Sorghum	cytosol	98.82	98.82
TraesCS4A01G298700.1	Wheat	cytosol	94.51	94.39
HORVU4Hr1G002270.3	Barley	cytosol	94.51	94.39
Zm00001d013644_P001	Maize	extracellular	97.78	93.85
Zm00001d031128_P004	Maize	plasma membrane, plastid	92.29	92.17
GSMUA_Achr5P02740_001	Banana	cytosol	78.3	89.4
GSMUA_Achr4P22700_001	Banana	cytosol	77.91	88.96
GSMUA_Achr7P01530_001	Banana	cytosol	88.76	88.64
Solyc10g081510.1.1	Tomato	extracellular	87.84	87.84
CDX85436	Canola	cytosol	87.45	87.45
TraesCS4B01G014700.2	Wheat	vacuole	94.38	87.41
PGSC0003DMT400072279	Potato	cytosol, extracellular	87.32	87.32
CDY02674	Canola	cytosol	87.32	87.32
CDY38470	Canola	cytosol	87.32	87.32
Bra023645.1-P	Field mustard	cytosol	87.32	87.32
AT5G17920.1	Thale cress	cytosol	87.19	87.19
Bra002116.1-P	Field mustard	cytosol	87.19	87.19
CDY25477	Canola	cytosol	87.19	87.19
AT3G03780.2	Thale cress	cytosol	87.19	87.19
TraesCS4D01G012900.1	Wheat	mitochondrion	94.12	87.17
CDX78767	Canola	cytosol	87.06	87.06
CDX74207	Canola	cytosol	86.93	86.93
Bra006429.1-P	Field mustard	cytosol	86.93	86.93
CDX70785	Canola	cytosol	86.8	86.8
KRG94871	Soybean	cytosol	86.54	86.76
PGSC0003DMT400022467	Potato	cytosol	86.67	86.67
KRH06666	Soybean	nucleus	86.41	86.63
CDX92051	Canola	cytosol	86.41	86.41
KRH57885	Soybean	cytosol	86.01	86.01
KRH04756	Soybean	cytosol, endoplasmic reticulum, nucleus	85.88	85.88
CDY50662	Canola	cytosol	83.79	85.13
Bra001090.1-P	Field mustard	cytosol	87.06	84.41
KRH04805	Soybean	plastid	46.14	84.25
GSMUA_Achr4P21470_001	Banana	mitochondrion	88.63	83.39
KRG89918	Soybean	endoplasmic reticulum	86.27	82.6
KRH17939	Soybean	nucleus	86.27	82.19
Solyc01g009180.2.1	Tomato	plastid	87.06	81.52
KRH63013	Soybean	cytosol	11.9	79.82
KRH04804	Soybean	endoplasmic reticulum	34.9	74.37
AT5G20980.2	Thale cress	mitochondrion	77.78	73.28
CDX70955	Canola	mitochondrion	78.04	73.25
CDX88830	Canola	mitochondrion	78.04	73.25
Bra006589.1-P	Field mustard	mitochondrion, plastid	77.91	73.13
KRH04683	Soybean	mitochondrion	25.23	69.18
CDX92419	Canola	plastid	77.52	67.69
CDY16584	Canola	plastid	77.25	62.54
KRH04746	Soybean	plastid	13.07	57.47
VIT_10s0042g00970.t01	Wine grape	cytosol	14.77	41.24

Protein Annotations

KEGG:00270+2.1.1.14	KEGG:00450+2.1.1.14	EntrezGene:100286153	Gene3D:3.20.20.210	MapMan:4.1.2.2.6.2.1	UniProt:B6UF55
InterPro:Cbl-indep_Met_Synth_N	InterPro:Cobalamin-indep_Met_synthase	EMBL:EU975870	GO:GO:0003674	GO:GO:0003824	GO:GO:0003871
GO:GO:0005488	GO:GO:0008150	GO:GO:0008152	GO:GO:0008168	GO:GO:0008270	GO:GO:0008652
GO:GO:0009058	GO:GO:0009086	GO:GO:0009987	GO:GO:0016740	GO:GO:0032259	InterPro:IPR038071
HAMAP:MF_00172	InterPro:Met_Synth_C/arc	ProteinID:ONM07669.1	PFAM:PF01717	PFAM:PF08267	PIRSF:PIRSF000382
PANTHER:PTHR30519	PANTHER:PTHR30519:SF11	SUPFAM:SSF51726	TIGRFAMs:TIGR01371	UniParc:UPI000182F780	InterPro:UROD/MetE-like_sf
EnsemblPlantsGene:Zm00001d033480	EnsemblPlants:Zm00001d033480_P002	EnsemblPlants:Zm00001d033480_T002	SEG:seg	:	:

Description

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Coordinates

chr1:-:263882342..263887652

Molecular Weight (calculated)

84474.9 Da

IEP (calculated)

6.106

GRAVY (calculated)

-0.143

Length

765 amino acids

Sequence
(BLAST)

001: MASHIVGYPR MGPKRELKFA LESFWDGKSS ADDLEKVASD LRSSIWKQMS EAGIKYIPSN TFSYYDQVLD TTAMLGAVPE RYSWTGGEIG LSTYFSMARG
101: NATVPAMEMT KWFDTNYHFI VPELGPSTKF TYASHKAVSE YKEAKALGID TVPVLVGPVS YLLLSKPAKG VEKSFSLLSL LGSILPIYKE VVSELKAAGA
201: SWIQFDEPTL VKDLDVHELA AFSSAYAELE SAFSGLNVLI ETYFADIPAE SYKTLTSLSG VTAYGFDLIR GAKTLDLIRS SFPSGKYLFA GVVDGRNIWA
301: DDLAASLSTL QSLEAVAGKD KLVVSTSCSL MHTAVDLVNE TKLDDEIKSW LAFAAQKVVE VNALAKALAG QKDEAYFAAN AAAQASRRSS PRVTNEEVQK
401: AAAALKGSDH RRATTVAARL DAQQKKLNLP VLPTTTIGSF PQTVELRRVR REYKAKKISE EEYISAIKEE ISKVVKIQEE LDIDVLVHGE PERNDMVEYF
501: GEQLSGFAFT ANGWVQSYGS RCVKPPIIYG DVSRPNPMTV FWSKTAQSMT ARPMKGMLTG PVTILNWSFV RNDQPRFETC YQIALAIKKE VEDLEAAGIQ
601: VIQIDEAALR EGLPLRKSEH AFYLDWAVHS FRITNCGVQD TTQIHTHMCY SNFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
701: HSPRIPSTEE IADRVNKMLA VLDTNILWVN PDCGLKTRKY TEVKPALTNM VSATKLIRTQ LGSAK

Best Arabidopsis Sequence Match ( AT3G03780.2 )
(BLAST)

001: MASHIVGYPR MGPKRELKFA LESFWDGKSS ADDLQKVSAD LRSDIWKQMS AAGIKYIPSN TFSHYDQVLD TTAMLGAVPS RYGFTSGEIG LDVYFSMARG
101: NASVPAMEMT KWFDTNYHYI VPELGPEVKF SYASHKAVNE YKEAKALGVE TVPVLVGPVS YLLLSKLAKG VDKSFDLLSL LPKILPVYKE VIAELKAAGA
201: SWIQLDEPLF VMDLEGHKLQ AFSGAYAELE STLSGLNVLV ETYFADIPAE AYKTLTSLKG VTAFGFDLVR GTKTIDLIKS GFPQGKYLFA GVVDGRNIWA
301: NDLAASLITL QSLEGVVGKD KLVVSTSCSL LHTAVDLINE TKLDAEIKSW LAFAAQKVVE VDALAKALAG QTNESFFTAN ADALSSRRSS PRVTNESVQK
401: AAAALKGSDH RRTTEVSARL DAQQKKLNLP ILPTTTIGSF PQTVELRRVR REYKAKKISE EDYVKAIKEE IKKVVDIQED LDIDVLVHGE PERNDMVEYF
501: GEQLSGFAFT ANGWVQSYGS RCVKPPVIYG DVSRPKPMTV FWSSTAQSMT KRPMKGMLTG PVTILNWSFV RNDQPRHETC YQIALAIKDE VEDLEKGGIG
601: VIQIDEAALR EGLPLRKAEH SFYLDWAVHS FRITNCGVQD STQIHTHMCY SNFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
701: HSPRIPSTDE IADRINKMLA VLEQNILWVN PDCGLKTRKY TEVKPALKAM VDAAKLIRSQ LGSAK

Arabidopsis Description

MS25-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9SRV5]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.