Factsheet for PGSC0003DMT400072279

Subcellular Localization

min:

: max

Winner_takes_all: extracellular, cytosol

Predictor Summary:

cytosol 4
plastid 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

cytosol: 22313117
extracellular: 22313117
extracellular: 28248230

msms PMID: 28248230 doi

E Elagamey, K Narula, A Sinha, PR Aggarwal, S Ghosh, N Chakraborty, S Chakraborty

National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. aggarwal.pooja28@yahoo.com., National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. arunima35@gmail.com., National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. gayatrinarula@gmail.com., National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. nchakraborty@nipgr.ac.in., National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. schakraborty@nipgr.ac.in., National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. sgblackpearl@gmail.com., National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. subhrac@hotmail.com.

msms PMID: 22313117 doi

S Lim, K Chisholm, RH Coffin, RD Peters, KI Al-Mughrabi, G Wang-Pruski, DM Pinto

Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc10g081510.1.1	Tomato	extracellular	98.3	98.3
AT5G17920.1	Thale cress	cytosol	89.15	89.15
CDY02674	Canola	cytosol	88.76	88.76
CDX85436	Canola	cytosol	88.76	88.76
KXG24060	Sorghum	cytosol	88.63	88.51
Zm00001d031128_P004	Maize	plasma membrane, plastid	88.63	88.51
Bra023645.1-P	Field mustard	cytosol	88.5	88.5
CDY38470	Canola	cytosol	88.5	88.5
KRH06666	Soybean	nucleus	88.24	88.47
KRG94871	Soybean	cytosol	88.24	88.47
CDX78767	Canola	cytosol	88.37	88.37
CDY25477	Canola	cytosol	88.37	88.37
CDX74207	Canola	cytosol	88.37	88.37
Bra002116.1-P	Field mustard	cytosol	88.37	88.37
AT3G03780.2	Thale cress	cytosol	88.37	88.37
Bra006429.1-P	Field mustard	cytosol	88.24	88.24
CDX70785	Canola	cytosol	88.1	88.1
CDX92051	Canola	cytosol	87.84	87.84
Os12t0623900-01	Rice	extracellular	87.84	87.73
TraesCS4A01G298700.1	Wheat	cytosol	87.71	87.6
Os12t0624000-02	Rice	extracellular	87.71	87.6
GSMUA_Achr4P22700_001	Banana	cytosol	76.6	87.46
KXG37902	Sorghum	cytosol	87.45	87.45
Zm00001d033480_P002	Maize	extracellular	87.32	87.32
HORVU4Hr1G002270.3	Barley	cytosol	87.32	87.21
GSMUA_Achr7P01530_001	Banana	cytosol	87.32	87.21
KRH04756	Soybean	cytosol, endoplasmic reticulum, nucleus	87.19	87.19
KRH57885	Soybean	cytosol	87.06	87.06
GSMUA_Achr5P02740_001	Banana	cytosol	76.21	87.01
PGSC0003DMT400022467	Potato	cytosol	86.54	86.54
CDY50662	Canola	cytosol	84.84	86.19
TraesCS5D01G031300.2	Wheat	golgi, mitochondrion, nucleus, plasma membrane, unclear	86.67	86.1
TraesCS5B01G022800.4	Wheat	extracellular, golgi, plastid, unclear	86.8	86.01
Bra001090.1-P	Field mustard	cytosol	87.84	85.17
KRH04805	Soybean	plastid	46.27	84.49
Zm00001d013644_P001	Maize	extracellular	87.32	83.81
HORVU5Hr1G006780.7	Barley	cytosol	50.2	82.23
TraesCS5A01G024900.2	Wheat	cytosol	86.67	82.16
GSMUA_Achr4P21470_001	Banana	mitochondrion	87.19	82.04
TraesCS4B01G014700.2	Wheat	vacuole	87.58	81.11
TraesCS4D01G012900.1	Wheat	mitochondrion	87.45	80.99
KRH63013	Soybean	cytosol	11.9	79.82
KRH04804	Soybean	endoplasmic reticulum	36.08	76.88
KRH04746	Soybean	plastid	13.33	58.62

Protein Annotations

KEGG:00270+2.1.1.14	KEGG:00450+2.1.1.14	Gene3D:3.20.20.210	MapMan:4.1.2.2.6.2.1	ProteinID:AAF74983	ProteinID:AAF74983.1
EMBL:AF082893	InterPro:Cbl-indep_Met_Synth_N	InterPro:Cobalamin-indep_Met_synthase	GO:GO:0003674	GO:GO:0003824	GO:GO:0003871
GO:GO:0005488	GO:GO:0008150	GO:GO:0008152	GO:GO:0008168	GO:GO:0008270	GO:GO:0008652
GO:GO:0009058	GO:GO:0009086	GO:GO:0009987	GO:GO:0016740	GO:GO:0032259	InterPro:IPR038071
UniProt:M1CQ02	HAMAP:MF_00172	InterPro:Met_Synth_C/arc	PFAM:PF01717	PFAM:PF08267	EnsemblPlantsGene:PGSC0003DMG400028127
PGSC:PGSC0003DMG400028127	EnsemblPlants:PGSC0003DMT400072279	PIRSF:PIRSF000382	PANTHER:PTHR30519	PANTHER:PTHR30519:SF11	SUPFAM:SSF51726
TIGRFAMs:TIGR01371	UniParc:UPI000295AF0A	InterPro:UROD/MetE-like_sf	SEG:seg	:	:

Description

Methionine synthase [Source:PGSC_GENE;Acc:PGSC0003DMG400028127]

Coordinates

chr10:-:56459089..56462664

Molecular Weight (calculated)

84602.2 Da

IEP (calculated)

6.590

GRAVY (calculated)

-0.170

Length

765 amino acids

Sequence
(BLAST)

001: MASHVVGYPR MGPKRELKFA LESFWDGKSS AEDLKKVSAD LRSSIWKQMS DAGIKYIPSN TFSYYDQVLD TTTMLGAVPS RYNWTGGEIE FGTYFSMARG
101: NASVPAMEMT KWFDTNYHFI VPELGPDVNF SYASHKAVNE YKEAKAQGVD TVPVLVGPVS YLLLSKPAKG VDKSFPLLSL LDKILPIYKE VIAELKAAGA
201: SWIQLDEPTL VLDLESHKLE AFTKAYADLE SSLSGLNVLV ETYFADVPAG AFKTLTALKG VTAFGFDLVR GTQTLELIKS SFPSGKYLFA GVVDGRNIWA
301: SDLAASLALL QSLEGVVGKD KLVTSTSCSL LHTAVDLINE TKLDDEIKSW LAFAAQKVVE VNALAKALSG AKDEAFFSAN AAAQASRKSS PRVTNEAVRK
401: ASAALQGSDH RRATNVSARL DAQQKKLNLP ILPTTTIGSF PQTVELRRVR REYKAKKISE EEYVKAITEE IKKVVDLQEE LDIDVLVHGE PERNDMVEYF
501: GEQLSGFAFT ANGWVQSYGS RCVKPPIIYG DVSRPKAMTV FWSSKAQEMT KRPMKGMLTG PVTILNWSFV RNDQPRFETC YQIALAIKDE VEDLEKAGIT
601: VIQIDEAALR EGLPLRKAEH TSYLNWAVHS FRITNVGIQD TTQIHTHMCY SNFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
701: HSPRIPSTEE IADRVNKMLA VLETNILWVN PDCGLKTRKY TEVKPALQNM VSAAKTIRTQ LASAK

Best Arabidopsis Sequence Match ( AT5G17920.2 )
(BLAST)

001: MASHIVGYPR MGPKRELKFA LESFWDGKST AEDLQKVSAD LRSSIWKQMS AAGTKFIPSN TFAHYDQVLD TTAMLGAVPP RYGYTGGEIG LDVYFSMARG
101: NASVPAMEMT KWFDTNYHYI VPELGPEVNF SYASHKAVNE YKEAKALGVD TVPVLVGPVS YLLLSKAAKG VDKSFELLSL LPKILPIYKE VITELKAAGA
201: TWIQLDEPVL VMDLEGQKLQ AFTGAYAELE STLSGLNVLV ETYFADIPAE AYKTLTSLKG VTAFGFDLVR GTKTLDLVKA GFPEGKYLFA GVVDGRNIWA
301: NDFAASLSTL QALEGIVGKD KLVVSTSCSL LHTAVDLINE TKLDDEIKSW LAFAAQKVVE VNALAKALAG QKDEALFSAN AAALASRRSS PRVTNEGVQK
401: AAAALKGSDH RRATNVSARL DAQQKKLNLP ILPTTTIGSF PQTVELRRVR REYKAKKVSE EDYVKAIKEE IKKVVDLQEE LDIDVLVHGE PERNDMVEYF
501: GEQLSGFAFT ANGWVQSYGS RCVKPPVIYG DVSRPKAMTV FWSAMAQSMT SRPMKGMLTG PVTILNWSFV RNDQPRHETC YQIALAIKDE VEDLEKGGIG
601: VIQIDEAALR EGLPLRKSEH AFYLDWAVHS FRITNCGVQD STQIHTHMCY SHFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
701: HSPRIPSSEE IADRVNKMLA VLEQNILWVN PDCGLKTRKY TEVKPALKNM VDAAKLIRSQ LASAK

Arabidopsis Description

MS15-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 [Source:UniProtKB/Swiss-Prot;Acc:O50008]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.