Factsheet for Zm00001d031128_P004

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, plastid

Predictor Summary:

cytosol 2
mitochondrion 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane, plastid
Any Predictor:	cytosol, mitochondrion
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	mitochondrion

plasma membrane: 18521681
plastid: 20089766
extracellular: 20408568
plastid: 22065420
extracellular: 22192489
plastid: 23198870
plasma membrane: 23508561
mitochondrion: 27297264
plasma membrane: 27341663

msms PMID: 27297264 doi

D Dahal, KJ Newton, BP Mooney

Division of Biological Sciences, ‡Department of Biochemistry, and §The Charles W Gehrke Proteomics Center, University of Missouri , Columbia, Missouri 65211, United States.

msms PMID: 20089766 doi

G Friso, W Majeran, M Huang, Q Sun, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA.

msms PMID: 22192489 doi

K Witzel, M Shahzad, A Matros, HP Mock, KH Mühling

Institute of Plant Nutrition and Soil Science, Christian Albrechts University, Hermann-Rodewald-Strasse 2, 24118 Kiel, Germany. khmuehling@plantnutrition.uni-kiel.de.

msms PMID: 23198870 doi

M Huang, G Friso, K Nishimura, X Qu, PD Olinares, W Majeran, Q Sun, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, United States.

msms PMID: 18521681 doi

P Carletti, A Masi, B Spolaore, P Polverino De Laureto, M De Zorzi, L Turetta, M Ferretti, S Nardi

Department of Agricultural Biotechnology, University of Padua, Agripolis, Strada Romea, 16-35020 Legnaro, Padova, Italy.

msms PMID: 27341663 doi

P Voothuluru, JC Anderson, RE Sharp, SC Peck

Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA., Division of Biochemistry, University of Missouri, Columbia, MO, 65211, USA., Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA., Interdisciplinary Plant Group, University of Missouri, Columbia, MO, 65211, USA.

msms PMID: 20408568 doi

W Ma, N Muthreich, C Liao, M Franz-Wachtel, W Schütz, F Zhang, F Hochholdinger, C Li

Department of Plant Nutrition, China Agricultural University, Beijing, PR China.

msms PMID: 22065420 doi

W Majeran, G Friso, Y Asakura, X Qu, M Huang, L Ponnala, KP Watkins, A Barkan, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA.

msms PMID: 23508561 doi

Z Zhang, P Voothuluru, M Yamaguchi, RE Sharp, SC Peck

Division of Biochemistry, University of Missouri Columbia, MO, USA ; Christopher S. Bond Life Sciences Center, University of Missouri Columbia, MO, USA ; Interdisciplinary Plant Group, University of Missouri Columbia, MO, USA.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KXG24060	Sorghum	cytosol	97.78	97.78
Os12t0624000-02	Rice	extracellular	94.52	94.52
Os12t0623900-01	Rice	extracellular	94.52	94.52
Zm00001d033480_P002	Maize	extracellular	92.17	92.29
GSMUA_Achr4P22700_001	Banana	cytosol	78.59	89.85
GSMUA_Achr7P01530_001	Banana	cytosol	89.3	89.3
Solyc10g081510.1.1	Tomato	extracellular	89.16	89.28
GSMUA_Achr5P02740_001	Banana	cytosol	78.07	89.25
PGSC0003DMT400072279	Potato	cytosol, extracellular	88.51	88.63
Zm00001d013644_P001	Maize	extracellular	91.91	88.33
CDY38470	Canola	cytosol	87.99	88.1
CDX85436	Canola	cytosol	87.86	87.97
Bra023645.1-P	Field mustard	cytosol	87.73	87.84
AT5G17920.1	Thale cress	cytosol	87.6	87.71
Bra002116.1-P	Field mustard	cytosol	87.6	87.71
CDY25477	Canola	cytosol	87.6	87.71
CDY02674	Canola	cytosol	87.6	87.71
CDX78767	Canola	cytosol	87.47	87.58
Bra006429.1-P	Field mustard	cytosol	87.34	87.45
AT3G03780.2	Thale cress	cytosol	87.21	87.32
CDX70785	Canola	cytosol	87.21	87.32
KRH06666	Soybean	nucleus	86.95	87.29
CDX74207	Canola	cytosol	87.08	87.19
KRG94871	Soybean	cytosol	86.81	87.16
PGSC0003DMT400022467	Potato	cytosol	86.81	86.93
CDX92051	Canola	cytosol	86.81	86.93
KRH04756	Soybean	cytosol, endoplasmic reticulum, nucleus	85.51	85.62
KRH57885	Soybean	cytosol	85.51	85.62
CDY50662	Canola	cytosol	83.81	85.26
Bra001090.1-P	Field mustard	cytosol	86.55	84.03
GSMUA_Achr4P21470_001	Banana	mitochondrion	89.16	84.01
KRH04805	Soybean	plastid	45.82	83.77
KRH17939	Soybean	nucleus	86.16	82.19
KRG89918	Soybean	endoplasmic reticulum	85.38	81.85
Solyc01g009180.2.1	Tomato	plastid	87.21	81.76
KRH63013	Soybean	cytosol	11.88	79.82
KRH04804	Soybean	endoplasmic reticulum	35.12	74.93
CDX70955	Canola	mitochondrion	78.2	73.5
CDX88830	Canola	mitochondrion	78.07	73.37
Bra006589.1-P	Field mustard	mitochondrion, plastid	78.07	73.37
AT5G20980.2	Thale cress	mitochondrion	77.68	73.28
KRH04683	Soybean	mitochondrion	25.46	69.89
CDX92419	Canola	plastid	77.81	68.04
CDY16584	Canola	plastid	77.28	62.65
KRH04746	Soybean	plastid	12.92	56.9

Protein Annotations

KEGG:00270+2.1.1.14	KEGG:00450+2.1.1.14	Gene3D:3.20.20.210	MapMan:4.1.2.2.6.2.1	EntrezGene:541942	EMBL:BT063702
UniProt:C0P5Y3	InterPro:Cbl-indep_Met_Synth_N	InterPro:Cobalamin-indep_Met_synthase	GO:GO:0003674	GO:GO:0003824	GO:GO:0003871
GO:GO:0005488	GO:GO:0008150	GO:GO:0008152	GO:GO:0008168	GO:GO:0008270	GO:GO:0008652
GO:GO:0009058	GO:GO:0009086	GO:GO:0009987	GO:GO:0016740	GO:GO:0032259	InterPro:IPR038071
HAMAP:MF_00172	InterPro:Met_Synth_C/arc	ProteinID:ONM02225.1	PFAM:PF01717	PFAM:PF08267	PIRSF:PIRSF000382
PANTHER:PTHR30519	PANTHER:PTHR30519:SF11	SUPFAM:SSF51726	TIGRFAMs:TIGR01371	UniParc:UPI0001948E99	InterPro:UROD/MetE-like_sf
EnsemblPlantsGene:Zm00001d031128	EnsemblPlants:Zm00001d031128_P004	EnsemblPlants:Zm00001d031128_T004	SEG:seg	:	:

Description

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1

Coordinates

chr1:+:178799384..178803593

Molecular Weight (calculated)

84496.9 Da

IEP (calculated)

5.498

GRAVY (calculated)

-0.131

Length

766 amino acids

Sequence
(BLAST)

001: MASHIVGYPR MGPKRELKFA LESFWDGKST AEDLEKVATD LRASIWKQMA DAGIKYIPSN TFSYYDQVLD TTAMLGAVPE RYSWTGGEIG FDTYFSMARG
101: NATVPAMEMT KWFDTNYHFI VPELGPNTKF SYASHKAVNE YKEAKALGVD TVPVLVGPVS YLLLSKPAKG VEKGFPLLSL LSSILPVYKE VIAELKAAGA
201: SWIQFDEPTL VLDLDSDKLA AFSAAYAELE SVLSGLNVLV ETYFADVPAE SYKTLTSLSS VTAYGFDLVR GTQTLGLVTS AGFPAGKYLF AGVVDGRNIW
301: ADDLATSLST LQSLEAVVGK DKLVVSTSCS LMHTAVDLVN ETKLDSEIKS WLAFAAQKVV EVDALAKALA GQKDEAYFAA NAAAQASRKS SPRVTNEEVQ
401: KAAAALKGSD HRRGTNVSAR LDAQQKKLNL PILPTTTIGS FPQTVELRRV RREYKAKKIS EEEYVTAIKE EINKVVKLQE ELDIDVLVHG EPERNDMVEY
501: FGEQLSGFAF TANGWVQSYG SRCVKPPIIY GDVSRPNPMT VFWSKTAQSM TSRPMKGMLT GPVTILNWSF VRNDQPRFET CYQIALAIKK EVEDLEAGGI
601: QVIQIDEAAL REGLPLRKAE HAFYLDWAVH SFRITNCEIQ DTTQIHTHMC YSNFNDIIHS IIDMDADVIT IENSRSDEKL LSVFREGVKY GAGIGPGVYD
701: IHSPRIPSAE EIADRIDKML AVLDTNILWV NPDCGLKTRK YTEVKPALTN MVSAAKLIRT QLASAK

Best Arabidopsis Sequence Match ( AT5G17920.2 )
(BLAST)

001: MASHIVGYPR MGPKRELKFA LESFWDGKST AEDLQKVSAD LRSSIWKQMS AAGTKFIPSN TFAHYDQVLD TTAMLGAVPP RYGYTGGEIG LDVYFSMARG
101: NASVPAMEMT KWFDTNYHYI VPELGPEVNF SYASHKAVNE YKEAKALGVD TVPVLVGPVS YLLLSKAAKG VDKSFELLSL LPKILPIYKE VITELKAAGA
201: TWIQLDEPVL VMDLEGQKLQ AFTGAYAELE STLSGLNVLV ETYFADIPAE AYKTLTSLKG VTAFGFDLVR GTKTLDLVKA GFPEGKYLFA GVVDGRNIWA
301: NDFAASLSTL QALEGIVGKD KLVVSTSCSL LHTAVDLINE TKLDDEIKSW LAFAAQKVVE VNALAKALAG QKDEALFSAN AAALASRRSS PRVTNEGVQK
401: AAAALKGSDH RRATNVSARL DAQQKKLNLP ILPTTTIGSF PQTVELRRVR REYKAKKVSE EDYVKAIKEE IKKVVDLQEE LDIDVLVHGE PERNDMVEYF
501: GEQLSGFAFT ANGWVQSYGS RCVKPPVIYG DVSRPKAMTV FWSAMAQSMT SRPMKGMLTG PVTILNWSFV RNDQPRHETC YQIALAIKDE VEDLEKGGIG
601: VIQIDEAALR EGLPLRKSEH AFYLDWAVHS FRITNCGVQD STQIHTHMCY SHFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
701: HSPRIPSSEE IADRVNKMLA VLEQNILWVN PDCGLKTRKY TEVKPALKNM VDAAKLIRSQ LASAK

Arabidopsis Description

MS15-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 [Source:UniProtKB/Swiss-Prot;Acc:O50008]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.