CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 2
mitochondrion 4
nucleus 1
extracellular 1
golgi 2
plasma membrane 1
endoplasmic reticulum 1
vacuole 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	mitochondrion, nucleus, plastid, secretory
BaCelLo:	plastid
iPSORT:	mitochondrion
MultiLoc:	mitochondrion
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	mitochondrion
WoLF PSORT:	plastid
YLoc:	golgi

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH43643	Soybean	nucleus	94.31	94.31
VIT_02s0025g04190.t01	Wine grape	endoplasmic reticulum, vacuole	69.57	70.2
Solyc08g080550.1.1	Tomato	nucleus	64.59	66.24
PGSC0003DMT400007864	Potato	golgi, mitochondrion, plastid	63.88	65.51
AT1G12770.2	Thale cress	mitochondrion, plastid	59.25	60.44
Os02t0636300-01	Rice	nucleus	60.32	59.16
Os03t0219700-01	Rice	plastid	60.14	58.99
OQU85369	Sorghum	mitochondrion, plastid	59.43	58.29
HORVU6Hr1G059910.1	Barley	mitochondrion	58.9	55.07
TraesCS6A01G234000.1	Wheat	mitochondrion	58.72	54.91
GSMUA_Achr1P01890_001	Banana	plastid	51.42	54.63
TraesCS6B01G262800.1	Wheat	mitochondrion	58.36	54.58
TraesCS6D01G216300.1	Wheat	mitochondrion	51.96	54.28
Zm00001d017345_P002	Maize	plastid	59.43	52.6
KRH12197	Soybean	nucleus	20.28	28.08
KRH37217	Soybean	endoplasmic reticulum	20.28	28.01
KRH37426	Soybean	cytosol, endoplasmic reticulum	20.46	27.84
KRH12430	Soybean	cytosol	20.46	27.84
KRH02682	Soybean	cytosol, endoplasmic reticulum, nucleus	20.28	27.6
KRH52210	Soybean	cytosol, endoplasmic reticulum	20.28	27.6
KRH61534	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	20.28	27.6
KRH19215	Soybean	cytosol, endoplasmic reticulum, nucleus	20.28	27.6
KRH47074	Soybean	nucleus	19.93	27.12
KRH10048	Soybean	cytosol, endoplasmic reticulum	19.93	27.12
KRH23270	Soybean	cytosol	19.93	27.12
KRH44102	Soybean	nucleus	19.93	26.6
KRH04755	Soybean	cytosol	12.46	26.42
KRH44441	Soybean	cytosol, nucleus	19.75	26.06
KRH47464	Soybean	nucleus	19.75	26.06
KRH52452	Soybean	nucleus	19.75	26.0
KRH61783	Soybean	cytosol	19.75	26.0
KRH51049	Soybean	plastid	20.64	24.58
KRG93607	Soybean	nucleus	21.0	23.84
KRG99590	Soybean	endoplasmic reticulum, nucleus	20.64	23.77
KRH02098	Soybean	cytosol	9.43	23.04
KRH48173	Soybean	mitochondrion	18.86	21.2
KRH48170	Soybean	endoplasmic reticulum	18.86	21.07
KRH65098	Soybean	endoplasmic reticulum, nucleus	18.68	20.92
KRH65096	Soybean	nucleus	18.51	20.84
KRH40488	Soybean	cytosol	18.33	19.11

Protein Annotations

EntrezGene:100812968	Gene3D:3.40.50.300	MapMan:35.1	EMBL:ACUP02009789	InterPro:DEAD/DEAH_box_helicase_dom	EnsemblPlantsGene:GLYMA_15G265600
GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003824	GO:GO:0004004	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005730
GO:GO:0005737	GO:GO:0006139	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0010501
GO:GO:0016787	InterPro:Helicase_ATP-bd	InterPro:Helicase_C	UniProt:I1MJG2	InterPro:IPR001650	InterPro:IPR014001
InterPro:IPR014014	EnsemblPlants:KRH13813	ProteinID:KRH13813	ProteinID:KRH13813.1	InterPro:P-loop_NTPase	PFAM:PF00270
PFAM:PF00271	PFscan:PS51192	PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF537
InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487	SMART:SM00490	SUPFAM:SSF52540	UniParc:UPI000233BE80	SEG:seg

Description

hypothetical protein

Coordinates

chr15:-:50070458..50072776

Molecular Weight (calculated)

61570.7 Da

IEP (calculated)

10.140

GRAVY (calculated)

-0.247

Length

562 amino acids

Sequence
(BLAST)

001: MPTLISYRFL LLVGESLHMR RALATSRSVL LHTSVKCMSQ VEPHHGSLTV SGIGLQTETE PRNRSKTNKV KPLGSPSENR KSKGKPLGIN EKKKKAFRVV
101: EKQQIESAPF AAESFSELGL PHVLIERLEK EGFTVPTEVQ SAAVPTILNN HDVIIQSYTG SGKTLAYLLP ILSVVGPLRG KTPEGNSDGG ESGKKLGIEA
201: VIVAPSRELG MQIVREFEKV LGMDNKRVVQ QLVGGANRTR QEDALKKNKP AIVVGTPGRI AELSASGKLR THGCRYLVLD EVDELLSFNF REDMHRILEH
301: VGRRSGADPN SDSRKAERQL IMVSATVPFS VVRAARSWGC DPLLVQANKV APLETVSPSE PISLSRSSPS SSPSSAMPSP AAVESLPPAL KHYYFVTRVQ
401: HKVDVLRRCI HALDAKFVIA FMNHTKQLKD VVFKLEARGM KAMELHGDLG KLARSTTLKK FKNGEVRVLV TNELSARGLD VAECDLVVNL DLPTDSIHYA
501: HRAGRTGRLG RNGTVVTICE ESEVFVVKKL QKQLAIPIAA CDFAEGKFLV TEEEKTVSTS QR

Best Arabidopsis Sequence Match ( AT1G12770.1 )
(BLAST)

001: MAASTSTRFL VLLKDFSAFR KISWTCAATN FHRQSRFLCH VAKEDGSLTL ASLDLGNKPR KFGKGKAMKL EGSFVTEMGQ GKVRAVKNDK MKVVKEKKPA
101: EIVSPLFSAK SFEELGLPDS LLDSLEREGF SVPTDVQSAA VPAIIKGHDA VIQSYTGSGK TLAYLLPILS EIGPLAEKSR SSHSENDKRT EIQAMIVAPS
201: RELGMQIVRE VEKLLGPVHR RMVQQLVGGA NRMRQEEALK KNKPAIVVGT PGRIAEISKG GKLHTHGCRF LVLDEVDELL SFNFREDIHR ILEHVGKRSG
301: AGPKGEVDER ANRQTILVSA TVPFSVIRAA KSWSHEPVLV QANKVTPLDT VQPSAPVMSL TPTTSEADGQ IQTTIQSLPP ALKHYYCISK HQHKVDTLRR
401: CVHALDAQSV IAFMNHSRQL KDVVYKLEAR GMNSAEMHGD LGKLGRSTVL KKFKNGEIKV LVTNELSARG LDVAECDLVV NLELPTDAVH YAHRAGRTGR
501: LGRKGTVVTV CEESQVFIVK KMEKQLGLPF LYCEFVDGEL VVTEEDKAII R

Arabidopsis Description

RH47DEAD-box ATP-dependent RNA helicase 47, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q8W4E1]
SUBAcon: [plastid,mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.