CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

plastid 1
nucleus 2
mitochondrion 4
cytosol 3

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH62823
KRH70630
KRH70633
KRH75529

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G45810.1	KRH62823	AT1G26110.1	21798944
AT2G45810.1	KRH70630	AT1G26110.1	21798944
AT2G45810.1	KRH70633	AT1G26110.1	21798944
AT2G45810.1	KRH75529	AT1G26110.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH04755	Soybean	cytosol	51.39	97.36
KRH48173	Soybean	mitochondrion	96.81	97.2
KRH65096	Soybean	nucleus	96.41	96.99
KRH48170	Soybean	endoplasmic reticulum	95.62	95.43
CDY54061	Canola	cytosol	58.96	90.8
CDY45702	Canola	cytosol	35.86	87.38
Bra008527.1-P	Field mustard	cytosol	56.97	86.67
CDY51147	Canola	cytosol	84.66	85.69
CDY07205	Canola	cytosol	83.27	85.13
AT4G00660.1	Thale cress	cytosol, mitochondrion	85.46	84.95
CDX91901	Canola	cytosol	84.26	84.43
Bra037351.1-P	Field mustard	cytosol	85.26	83.92
Zm00001d013910_P002	Maize	endoplasmic reticulum, vacuole	56.37	83.24
Os04t0533000-02	Rice	cytosol	82.47	83.13
CDX74342	Canola	cytosol	84.86	82.88
Os02t0641800-02	Rice	plasma membrane, plastid	83.86	82.87
Bra000965.1-P	Field mustard	cytosol	84.66	82.85
TraesCS2B01G387200.1	Wheat	cytosol	81.47	81.96
TraesCS2A01G370200.2	Wheat	cytosol	81.08	81.24
HORVU6Hr1G060630.1	Barley	cytosol	83.47	80.89
TraesCS2D01G366700.1	Wheat	cytosol	81.08	80.12
TraesCS6B01G265300.1	Wheat	cytosol	83.27	79.92
TraesCS6A01G236800.1	Wheat	cytosol	83.27	79.92
TraesCS6D01G219400.1	Wheat	cytosol	83.07	79.73
Zm00001d051268_P004	Maize	extracellular	82.87	79.69
Bra007587.1-P	Field mustard	cytosol	74.7	79.28
CDY58787	Canola	cytosol	76.1	79.25
Bra003442.1-P	Field mustard	cytosol	75.3	79.25
Zm00001d017384_P008	Maize	cytosol	82.67	79.05
CDX71756	Canola	cytosol	74.7	78.29
AT3G61240.1	Thale cress	cytosol	77.49	78.11
KRH40488	Soybean	cytosol	83.67	77.92
Zm00001d032526_P006	Maize	cytosol, mitochondrion, nucleus	77.89	77.73
CDY48935	Canola	cytosol, plastid	73.51	77.68
CDY37067	Canola	cytosol	78.88	77.65
EER91410	Sorghum	cytosol	78.29	77.51
Os10t0503700-01	Rice	plasma membrane	80.28	77.35
EER91411	Sorghum	cytosol	78.09	77.32
CDY27357	Canola	cytosol, plastid	77.89	76.82
Bra039312.1-P	Field mustard	cytosol	78.88	76.6
GSMUA_Achr11P...	Banana	cytosol	85.26	75.75
TraesCS1A01G174600.1	Wheat	cytosol	79.08	75.62
TraesCS1B01G189900.1	Wheat	cytosol	78.88	75.43
AT2G45810.1	Thale cress	cytosol, plastid	79.08	75.19
TraesCS1D01G164500.1	Wheat	cytosol	78.69	75.1
HORVU1Hr1G046020.1	Barley	cytosol	78.69	70.04
KRH61534	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	29.88	36.32
KRH52210	Soybean	cytosol, endoplasmic reticulum	29.88	36.32
KRH19215	Soybean	cytosol, endoplasmic reticulum, nucleus	29.88	36.32
KRH23270	Soybean	cytosol	29.88	36.32
KRH10048	Soybean	cytosol, endoplasmic reticulum	29.88	36.32
KRH12430	Soybean	cytosol	29.68	36.08
KRH37426	Soybean	cytosol, endoplasmic reticulum	29.68	36.08
KRH02682	Soybean	cytosol, endoplasmic reticulum, nucleus	29.68	36.08
KRH47074	Soybean	nucleus	29.68	36.08
KRH44102	Soybean	nucleus	29.88	35.63
KRH12197	Soybean	nucleus	28.69	35.47
KRH37217	Soybean	endoplasmic reticulum	28.69	35.38
KRH52452	Soybean	nucleus	29.28	34.43
KRH61783	Soybean	cytosol	29.28	34.43
KRH44441	Soybean	cytosol, nucleus	28.88	34.04
KRH47464	Soybean	nucleus	28.88	34.04
KRG99590	Soybean	endoplasmic reticulum, nucleus	26.89	27.66
KRG93607	Soybean	nucleus	26.49	26.87
KRH02098	Soybean	cytosol	9.56	20.87
KRH51049	Soybean	plastid	17.93	19.07
KRH43643	Soybean	nucleus	21.31	19.04
KRH13813	Soybean	nucleus	20.92	18.68

Protein Annotations

EntrezGene:106794117	Gene3D:3.40.50.300	MapMan:35.1	EMBL:ACUP02001311	InterPro:DEAD/DEAH_box_helicase_dom	EnsemblPlantsGene:GLYMA_03G013200
GO:GO:0000166	GO:GO:0000932	GO:GO:0003674	GO:GO:0003676	GO:GO:0003824	GO:GO:0004004
GO:GO:0004386	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005634	GO:GO:0005730	GO:GO:0005737	GO:GO:0006139	GO:GO:0006412	GO:GO:0006417
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0010494	GO:GO:0010501
GO:GO:0016043	GO:GO:0016787	GO:GO:0019538	GO:GO:0033962	InterPro:Helicase_ATP-bd	InterPro:Helicase_C
UniProt:I1JKB0	InterPro:IPR001650	InterPro:IPR014001	InterPro:IPR014014	EnsemblPlants:KRH65098	ProteinID:KRH65098
ProteinID:KRH65098.1	InterPro:P-loop_NTPase	PFAM:PF00270	PFAM:PF00271	ScanProsite:PS00039	PFscan:PS51192
PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF555	InterPro:RNA-helicase_DEAD-box_CS	InterPro:RNA_helicase_DEAD_Q_motif
SMART:SM00487	SMART:SM00490	SUPFAM:SSF52540	UniParc:UPI0002339BE3	SEG:seg	:

Description

hypothetical protein

Coordinates

chr3:+:1337139..1343939

Molecular Weight (calculated)

57542.4 Da

IEP (calculated)

8.647

GRAVY (calculated)

-0.428

Length

502 amino acids

Sequence
(BLAST)

001: MNHNNNNRAR YPPGMGIGRG GFNPNLGQNP SLNQNPNLNQ NHHAFQARPP YHHQQQQPQY VQRHLLQPPP PQQQQQWLRR DANAVDEVEK TVQSEPMDSS
101: SQDWKARLKI PPADTRYRTE DVTATKGNEF EDYFLKRELL MGIYEKGFER PSPIQEESIP IALTGSDILA RAKNGTGKTA AFCIPALEKI DQDNNVIQVV
201: ILVPTRELAL QTSQVCKELG KHLKIQVMVT TGGTSLKDDI MRLYQPVHLL VGTPGRILDL AKKGVCILKD CAMLVMDEAD KLLSPEFQPS IEQLIHFLPT
301: TRQILMFSAT FPVTVKDFKD RYLRKPYVIN LMDELTLKGI TQFYAFVEER QKVHCLNTLF SKLQINQSII FCNSVNRVEL LAKKITELGY SCFYIHAKML
401: QDHRNRVFHD FRNGACRNLV CTDLFTRGID IQAVNVVINF DFPKNAETYL HRVGRSGRFG HLGLAVNLIT YEDRFNLYRI EQELGTEIKQ IPPQIDQAIY
501: CR

Best Arabidopsis Sequence Match ( AT4G00660.2 )
(BLAST)

001: MNNRGRYPPG IGAGRGAFNP NPNYQSRSGY QQHPPPQYVQ RGNYAQNHQQ QFQQAPSQPH QYQQQQQQQQ QWLRRGQIPG GNSNGDAVVE VEKTVQSEVI
101: DPNSEDWKAR LKLPAPDTRY RTEDVTATKG NEFEDYFLKR ELLMGIYEKG FERPSPIQEE SIPIALTGRD ILARAKNGTG KTAAFCIPVL EKIDQDNNVI
201: QAVIIVPTRE LALQTSQVCK ELGKHLKIQV MVTTGGTSLK DDIMRLYQPV HLLVGTPGRI LDLTKKGVCV LKDCSVLVMD EADKLLSQEF QPSVEHLISF
301: LPESRQILMF SATFPVTVKD FKDRFLTNPY VINLMDELTL KGITQFYAFV EERQKIHCLN TLFSKLQINQ SIIFCNSVNR VELLAKKITE LGYSCFYIHA
401: KMLQDHRNRV FHDFRNGACR NLVCTDLFTR GIDIQAVNVV INFDFPKNAE TYLHRVGRSG RFGHLGLAVN LITYEDRFNL YRIEQELGTE IKQIPPHIDQ
501: AIYCQ

Arabidopsis Description

RH8DEAD-box ATP-dependent RNA helicase 8 [Source:UniProtKB/Swiss-Prot;Acc:Q8RXK6]
SUBAcon: [mitochondrion,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.