CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 4
plastid 1
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
EES02617

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G45810.1	EES02617	AT1G26110.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EER91411	Sorghum	cytosol	99.01	99.01
Zm00001d032526_P006	Maize	cytosol, mitochondrion, nucleus	95.27	96.02
Zm00001d013910_P002	Maize	endoplasmic reticulum, vacuole	61.34	91.47
Os10t0503700-01	Rice	plasma membrane	93.1	90.59
KRH04755	Soybean	cytosol	46.15	88.3
CDY45702	Canola	cytosol	34.91	85.92
CDY54061	Canola	cytosol	55.23	85.89
TraesCS1A01G174600.1	Wheat	cytosol	86.78	83.81
TraesCS1B01G189900.1	Wheat	cytosol	86.59	83.62
Bra008527.1-P	Field mustard	cytosol	54.24	83.33
TraesCS1D01G164500.1	Wheat	cytosol	86.39	83.27
Solyc10g017530.2.1	Tomato	nucleus	79.49	82.08
VIT_02s0025g03790.t01	Wine grape	cytosol, plastid	77.51	81.88
Solyc01g094350.2.1	Tomato	nucleus	77.51	78.92
KRH48170	Soybean	endoplasmic reticulum	77.91	78.53
KRH48173	Soybean	mitochondrion	77.32	78.4
KRH65098	Soybean	endoplasmic reticulum, nucleus	77.51	78.29
HORVU1Hr1G046020.1	Barley	cytosol	86.98	78.19
KRH65096	Soybean	nucleus	76.92	78.16
CDY07205	Canola	cytosol	75.54	78.0
AT4G00660.1	Thale cress	cytosol, mitochondrion	77.51	77.82
CDY51147	Canola	cytosol	75.74	77.42
CDY48935	Canola	cytosol, plastid	72.39	77.26
Bra007587.1-P	Field mustard	cytosol	71.99	77.17
Bra003442.1-P	Field mustard	cytosol	72.19	76.73
CDX91901	Canola	cytosol	75.74	76.65
CDY58787	Canola	cytosol	72.58	76.35
Bra037351.1-P	Field mustard	cytosol	76.53	76.08
CDX71756	Canola	cytosol	71.79	75.99
AT3G61240.1	Thale cress	cytosol	73.96	75.3
Bra000965.1-P	Field mustard	cytosol	76.13	75.24
CDX74342	Canola	cytosol	76.13	75.1
CDY37067	Canola	cytosol	73.77	73.33
CDY27357	Canola	cytosol, plastid	72.98	72.69
Bra039312.1-P	Field mustard	cytosol	73.77	72.34
AT2G45810.1	Thale cress	cytosol, plastid	73.96	71.02
GSMUA_Achr11P...	Banana	cytosol	78.5	70.44
Solyc05g048850.2.1	Tomato	mitochondrion	71.4	67.41
EES04512	Sorghum	cytosol	29.78	36.83
EES07819	Sorghum	cytosol	28.01	34.89
KXG22711	Sorghum	cytosol	28.6	33.8
KXG32708	Sorghum	cytosol	28.21	33.41
OQU76995	Sorghum	cytosol	29.98	29.4
EER95473	Sorghum	cytosol	24.85	25.25
KXG34209	Sorghum	plastid	18.74	21.44
OQU85369	Sorghum	mitochondrion, plastid	20.91	18.5
OQU84655	Sorghum	cytosol	24.65	16.34
KXG29825	Sorghum	cytosol	22.88	13.15

Protein Annotations

Gene3D:3.40.50.300	MapMan:35.1	EntrezGene:8067079	UniProt:C5WWE1	InterPro:DEAD/DEAH_box_helicase_dom	EnsemblPlants:EER91410
ProteinID:EER91410	ProteinID:EER91410.1	ProteinID:EER91412.2	GO:GO:0000166	GO:GO:0000932	GO:GO:0003674
GO:GO:0003676	GO:GO:0003824	GO:GO:0004004	GO:GO:0004386	GO:GO:0005488	GO:GO:0005524
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005730	GO:GO:0005737
GO:GO:0006139	GO:GO:0006412	GO:GO:0006417	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058
GO:GO:0009987	GO:GO:0010494	GO:GO:0010501	GO:GO:0016043	GO:GO:0016787	GO:GO:0019538
GO:GO:0033962	InterPro:Helicase_ATP-bd	InterPro:Helicase_C	InterPro:IPR001650	InterPro:IPR014001	InterPro:IPR014014
InterPro:P-loop_NTPase	PFAM:PF00270	PFAM:PF00271	ScanProsite:PS00039	PFscan:PS51192	PFscan:PS51194
PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF352	InterPro:RNA-helicase_DEAD-box_CS	InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487
SMART:SM00490	EnsemblPlantsGene:SORBI_3001G201400	SUPFAM:SSF52540	unigene:Sbi.6097	UniParc:UPI0001A8242A	RefSeq:XP_002464412.1
RefSeq:XP_002464414.1	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr1:+:18270117..18276701

Molecular Weight (calculated)

56677.0 Da

IEP (calculated)

8.506

GRAVY (calculated)

-0.392

Length

507 amino acids

Sequence
(BLAST)

001: MDQPRARYPP GYGSRGGGGA GRGGGGGNGG GGGGGGGNHN YYGRNPQPQQ QHHYQQQQQQ QQHVHRNSSH QQQWLRRDQA PAVAGAASGN AAAKTAPQLD
101: AIDSSSQDWK AQLNIPAPDT RYRTEDVTAT KGNEFEDYFL KRELLMGIYE KGFERPSPIQ EESIPIALTG SDILARAKNG TGKTAAFCIP ALEKIDPEKT
201: AIQVVILVPT RELALQTSQV CKELGKYLNI QVMVSTGGTS LKDDIMRLYQ PVHLLVGTPG RILDLTRKGI CVLKDCSMLV MDEADKLLAP EFQPSVEALI
301: HFLPPSRQLL MFSATFPVTV KEFKEKYLPR PYVINLMDEL TLKGITQYYA FVEERQKVHC LNTLFSKLQI NQSIIFCNSV NRVELLAKKI TELGYSCFYI
401: HAKMLQDHRN RVFHDFRNGA CRNLVCTDLF TRGIDIQAVN VVINFDFPKT SETYLHRVGR SGRYGHLGLA VNLITYEDRF NLYRIEQELG TEIKTIPPQI
501: DLAVYCQ

Best Arabidopsis Sequence Match ( AT4G00660.2 )
(BLAST)

001: MNNRGRYPPG IGAGRGAFNP NPNYQSRSGY QQHPPPQYVQ RGNYAQNHQQ QFQQAPSQPH QYQQQQQQQQ QWLRRGQIPG GNSNGDAVVE VEKTVQSEVI
101: DPNSEDWKAR LKLPAPDTRY RTEDVTATKG NEFEDYFLKR ELLMGIYEKG FERPSPIQEE SIPIALTGRD ILARAKNGTG KTAAFCIPVL EKIDQDNNVI
201: QAVIIVPTRE LALQTSQVCK ELGKHLKIQV MVTTGGTSLK DDIMRLYQPV HLLVGTPGRI LDLTKKGVCV LKDCSVLVMD EADKLLSQEF QPSVEHLISF
301: LPESRQILMF SATFPVTVKD FKDRFLTNPY VINLMDELTL KGITQFYAFV EERQKIHCLN TLFSKLQINQ SIIFCNSVNR VELLAKKITE LGYSCFYIHA
401: KMLQDHRNRV FHDFRNGACR NLVCTDLFTR GIDIQAVNVV INFDFPKNAE TYLHRVGRSG RFGHLGLAVN LITYEDRFNL YRIEQELGTE IKQIPPHIDQ
501: AIYCQ

Arabidopsis Description

RH8DEAD-box ATP-dependent RNA helicase 8 [Source:UniProtKB/Swiss-Prot;Acc:Q8RXK6]
SUBAcon: [mitochondrion,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.