CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion, endoplasmic reticulum, nucleus, cytosol

Predictor Summary:

nucleus 2
vacuole 1
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH03030
KRH55033
KRH63081
KRH72326

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G72730.1	KRH03030	AT5G57870.1	21798944
AT1G72730.1	KRH55033	AT5G57870.1	21798944
AT1G72730.1	KRH63081	AT5G57870.1	21798944
AT1G72730.1	KRH72326	AT5G57870.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH52210	Soybean	cytosol, endoplasmic reticulum	98.79	98.79
Solyc12g095990.1.1	Tomato	nucleus	95.88	95.88
Solyc12g096000.1.1	Tomato	cytosol, nucleus, unclear	91.28	95.69
Solyc08g062800.2.1	Tomato	extracellular, plastid	95.64	95.64
PGSC0003DMT400021166	Potato	cytosol	95.64	95.64
PGSC0003DMT400075339	Potato	cytosol	95.4	95.4
PGSC0003DMT400075336	Potato	cytosol	94.19	94.88
KRH12430	Soybean	cytosol	94.67	94.67
KRH37426	Soybean	cytosol, endoplasmic reticulum	94.43	94.43
KRH19215	Soybean	cytosol, endoplasmic reticulum, nucleus	93.7	93.7
KRH10048	Soybean	cytosol, endoplasmic reticulum	93.46	93.46
KRH02682	Soybean	cytosol, endoplasmic reticulum, nucleus	93.46	93.46
KRH23270	Soybean	cytosol	93.46	93.46
KRH47074	Soybean	nucleus	92.98	92.98
CDY08220	Canola	cytosol	92.49	92.72
Bra030893.1-P	Field mustard	cytosol	92.25	92.48
Bra014365.1-P	Field mustard	cytosol	92.25	92.48
CDY02469	Canola	cytosol	92.25	92.48
CDY10607	Canola	cytosol	92.01	92.46
CDY39079	Canola	cytosol	92.01	92.23
AT1G54270.1	Thale cress	cytosol	91.77	91.99
Bra027387.1-P	Field mustard	cytosol	91.77	91.99
Bra021517.1-P	Field mustard	cytosol	91.77	91.99
KRH44102	Soybean	nucleus	93.22	91.45
Bra001530.1-P	Field mustard	cytosol	92.01	91.35
CDY24057	Canola	cytosol	89.59	89.81
Bra037977.1-P	Field mustard	cytosol	89.59	89.81
Bra029124.1-P	Field mustard	cytosol	88.38	89.02
CDY32586	Canola	cytosol	81.36	88.89
CDY33073	Canola	cytosol	88.62	88.83
AT1G72730.1	Thale cress	cytosol	88.86	88.65
Solyc07g040750.1.1	Tomato	cytosol	88.14	88.14
AT3G13920.5	Thale cress	cytosol	89.35	88.07
CDY49861	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	90.31	87.35
Bra008045.1-P	Field mustard	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	90.31	87.35
CDY55205	Canola	cytosol	89.35	87.23
CDY67069	Canola	cytosol	21.31	87.13
CDX97528	Canola	cytosol	89.35	86.82
CDX82505	Canola	cytosol	88.62	85.51
VIT_04s0008g00990.t01	Wine grape	cytosol	25.42	85.37
VIT_04s0008g00980.t01	Wine grape	cytosol	17.19	84.52
CDY72259	Canola	cytosol	45.28	84.23
CDX75739	Canola	cytosol	88.86	84.17
CDY11154	Canola	cytosol	38.5	78.71
CDY54037	Canola	cytosol	42.62	65.92
CDY53867	Canola	cytosol	65.62	64.22
KRH37217	Soybean	endoplasmic reticulum	62.23	63.15
KRH12197	Soybean	nucleus	61.99	63.05
CDX72939	Canola	mitochondrion, nucleus	64.16	56.87
TraesCS6B01G217800.1	Wheat	cytosol	55.69	54.63
Bra016037.1-P	Field mustard	cytosol	89.59	50.89
GSMUA_Achr9P13950_001	Banana	mitochondrion	36.56	49.19
TraesCS6A01G186600.1	Wheat	cytosol	57.38	43.09
CDY68148	Canola	nucleus	64.65	36.13
HORVU7Hr1G073770.1	Barley	cytosol, nucleus	55.93	36.09
KRH44441	Soybean	cytosol, nucleus	36.08	34.98
KRH47464	Soybean	nucleus	36.08	34.98
KRH52452	Soybean	nucleus	36.08	34.89
KRH61783	Soybean	cytosol	36.08	34.89
KRH04755	Soybean	cytosol	22.28	34.72
KRH48173	Soybean	mitochondrion	36.32	30.0
KRH65098	Soybean	endoplasmic reticulum, nucleus	36.32	29.88
KRH65096	Soybean	nucleus	36.08	29.86
KRH48170	Soybean	endoplasmic reticulum	36.32	29.82
KRG99590	Soybean	endoplasmic reticulum, nucleus	33.66	28.48
OQU84655	Sorghum	cytosol	51.82	27.97
KRH40488	Soybean	cytosol	36.32	27.83
KRG93607	Soybean	nucleus	32.93	27.47
Os02t0221300-01	Rice	cytosol	56.66	26.56
Zm00001d016350_P001	Maize	vacuole	29.54	24.21
KXG29825	Sorghum	cytosol	48.43	22.68
KRH02098	Soybean	cytosol	12.59	22.61
Zm00001d016351_P002	Maize	nucleus	48.67	22.53
KRH51049	Soybean	plastid	24.7	21.61
KRH13813	Soybean	nucleus	27.6	20.28
KRH43643	Soybean	nucleus	27.36	20.11
TraesCS6A01G186800.1	Wheat	cytosol	58.84	19.07
TraesCS6D01G173700.3	Wheat	cytosol	59.08	18.15
TraesCS6B01G217700.1	Wheat	cytosol	57.63	17.83
CDY49860	Canola	cytosol	3.87	12.8

Protein Annotations

EntrezGene:100806638	MapMan:17.4.2.1	Gene3D:3.40.50.300	UniProt:A0A0R0K474	EMBL:ACUP02002171	InterPro:DEAD/DEAH_box_helicase_dom
EnsemblPlantsGene:GLYMA_04G052900	GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003824	GO:GO:0004386
GO:GO:0005488	GO:GO:0005524	GO:GO:0016787	InterPro:Helicase_ATP-bd	InterPro:Helicase_C	InterPro:IPR001650
InterPro:IPR014001	InterPro:IPR014014	EnsemblPlants:KRH61534	ProteinID:KRH61534	ProteinID:KRH61534.1	InterPro:P-loop_NTPase
PFAM:PF00270	PFAM:PF00271	ScanProsite:PS00039	PFscan:PS51192	PFscan:PS51194	PFscan:PS51195
PANTHER:PTHR24031	PANTHER:PTHR24031:SF294	InterPro:RNA-helicase_DEAD-box_CS	InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487	SMART:SM00490
SUPFAM:SSF52540	UniParc:UPI0001B63A7A	:	:	:	:

Description

hypothetical protein

Coordinates

chr4:-:4282935..4286063

Molecular Weight (calculated)

46682.1 Da

IEP (calculated)

5.098

GRAVY (calculated)

-0.214

Length

413 amino acids

Sequence
(BLAST)

001: MAGLAPEGSQ FDARQYDAKM NELLGTDGQD FFTSYDEVCE SFDAMGLQEN LLRGIYAYGF EKPSAIQQRG IVPFCKGLDV IQQAQSGTGK TATFCSGVLQ
101: QLDYSLVECQ ALVLAPTREL AQQIEKVMRA LGDYLGVKVH ACVGGTSVRE DQRILSSGVH VVVGTPGRVF DMLRRQSLRS DNIRMFVLDE ADEMLSRGFK
201: DQIYDIFQLL PPKIQVGVFS ATMPPEALEI TRKFMNKPVR ILVKRDELTL EGIKQFFVNV DKEDWKLETL CDLYETLAIT QSVIFVNTRR KVDWLTDKMR
301: SRDHTVSATH GDMDQNTRDI IMREFRSGSS RVLITTDLLA RGIDVQQVSL VINYDLPTQP ENYLHRIGRS GRFGRKGVAI NFVTGDDERM LFDIQKFYNV
401: QIEELPANVA DLI

Best Arabidopsis Sequence Match ( AT3G13920.1 )
(BLAST)

001: MAGSAPEGTQ FDARQFDQKL NEVLEGQDEF FTSYDDVHES FDAMGLQENL LRGIYAYGFE KPSAIQQRGI VPFCKGLDVI QQAQSGTGKT ATFCSGVLQQ
101: LDFSLIQCQA LVLAPTRELA QQIEKVMRAL GDYLGVKVHA CVGGTSVRED QRILQAGVHV VVGTPGRVFD MLKRQSLRAD NIKMFVLDEA DEMLSRGFKD
201: QIYDIFQLLP PKIQVGVFSA TMPPEALEIT RKFMSKPVRI LVKRDELTLE GIKQFYVNVE KEEWKLETLC DLYETLAITQ SVIFVNTRRK VDWLTDKMRS
301: RDHTVSATHG DMDQNTRDII MREFRSGSSR VLITTDLLAR GIDVQQVSLV INFDLPTQPE NYLHRIGRSG RFGRKGVAIN FVTRDDERML FDIQKFYNVV
401: VEELPSNVAD LL

Arabidopsis Description

EIF4A1Eukaryotic translation initiation factor 4A1 [Source:UniProtKB/TrEMBL;Acc:A0A1I9LSZ7]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.