CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

plastid 6
cytosol 3
nucleus 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG90357
KRG97402
KRG97711
KRH14933
KRH31163
KRH31506
KRH33614
KRH52210
KRH61534
KRH73205

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G24050.1	KRG97402	AT1G27360.1	21798944
AT2G24050.1	KRH31506	AT1G27360.1	21798944
AT2G24050.1	KRG97711	AT3G08530.1	21798944
AT2G24050.1	KRH14933	AT3G08530.1	21798944
AT2G24050.1	KRH31163	AT3G08530.1	21798944
AT2G24050.1	KRH73205	AT3G08530.1	21798944
AT2G24050.1	KRG90357	AT5G35620.1	21798944
AT2G24050.1	KRH33614	AT5G35620.1	21798944
AT5G57870.1	KRH52210	AT1G72730.1	21798944
AT5G57870.1	KRH61534	AT1G72730.1	21798944
AT5G57870.1	KRG97711	AT3G08530.1	21798944
AT5G57870.1	KRH14933	AT3G08530.1	21798944
AT5G57870.1	KRH31163	AT3G08530.1	21798944
AT5G57870.1	KRH73205	AT3G08530.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH03030	Soybean	endoplasmic reticulum	92.35	91.76
KRH55033	Soybean	endoplasmic reticulum	71.05	71.87
KRH63081	Soybean	endoplasmic reticulum	70.92	71.83
Solyc07g005810.2.1	Tomato	plastid	67.98	68.95
VIT_11s0037g00490.t01	Wine grape	plastid	69.26	68.39
GSMUA_Achr4P06000_001	Banana	cytosol	3.57	68.29
PGSC0003DMT400029102	Potato	cytosol, plastid	67.73	68.08
PGSC0003DMT400020464	Potato	cytosol	65.69	66.62
Solyc12g009960.1.1	Tomato	nucleus	64.92	65.85
CDY69274	Canola	cytosol, mitochondrion	26.79	64.22
GSMUA_Achr4P05990_001	Banana	cytosol	11.99	63.95
AT5G57870.1	Thale cress	plastid	63.01	63.33
CDY28211	Canola	mitochondrion	37.76	62.71
Bra011218.1-P	Field mustard	plastid	43.49	62.34
CDY28203	Canola	cytosol	40.56	62.23
CDY32742	Canola	plastid	60.2	61.3
Bra020407.1-P	Field mustard	plastid	60.08	61.17
CDX72262	Canola	cytosol	58.04	60.99
CDY39236	Canola	cytosol	57.4	60.89
GSMUA_Achr9P22750_001	Banana	plastid	57.53	60.62
GSMUA_AchrUn_...	Banana	cytosol	57.65	60.59
Bra024051.1-P	Field mustard	cytosol	57.65	60.43
CDY02774	Canola	plastid	59.44	60.36
CDX68687	Canola	plastid	56.63	59.36
Bra011211.1-P	Field mustard	plastid	56.51	59.15
Zm00001d025777_P007	Maize	plastid	58.93	58.85
EES12452	Sorghum	cytosol	59.31	58.79
Zm00001d003147_P001	Maize	plastid	58.67	58.52
HORVU2Hr1G086240.1	Barley	cytosol	55.48	57.77
AT2G24050.1	Thale cress	plastid	54.85	57.56
TraesCS2A01G354900.1	Wheat	cytosol	57.53	57.09
Os04t0499300-01	Rice	plastid	58.67	57.07
TraesCS2B01G373600.1	Wheat	cytosol	57.27	56.91
TraesCS2D01G353600.1	Wheat	cytosol, golgi, nucleus	57.14	56.85
Os02t0611500-01	Rice	cytosol	56.25	56.54
AT4G30680.1	Thale cress	mitochondrion, plastid	17.6	52.47
Solyc07g005780.1.1	Tomato	cytosol	28.32	49.33
GSMUA_Achr3P28040_001	Banana	cytosol	28.19	49.0
AT1G62410.1	Thale cress	cytosol	13.78	48.43
GSMUA_Achr4P05970_001	Banana	cytosol	8.16	42.38
GSMUA_Achr4P12750_001	Banana	cytosol	61.61	41.64
HORVU6Hr1G023180.1	Barley	cytosol	14.92	39.66
Os06t0520600-01	Rice	extracellular	20.28	22.36
KRH66436	Soybean	endoplasmic reticulum	25.0	11.75
KRH48862	Soybean	nucleus	24.74	11.59
KRH75579	Soybean	endoplasmic reticulum	25.26	10.87
KRH46542	Soybean	nucleus	24.87	10.62

Protein Annotations

Gene3D:1.25.40.180	EntrezGene:100789296	MapMan:17.4.2.4.2	EMBL:ACUP02001201	InterPro:ARM-type_fold	EnsemblPlantsGene:GLYMA_02G205500
GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0005488	GO:GO:0005515	UniProt:I1JGR4
InterPro:IPR003891	InterPro:IPR016021	InterPro:Initiation_fac_eIF4g_MI	EnsemblPlants:KRH72326	ProteinID:KRH72326	ProteinID:KRH72326.1
InterPro:MIF4-like_sf	InterPro:MIF4G-like_typ-3	PFAM:PF02847	PFAM:PF02854	PFscan:PS51366	PANTHER:PTHR23253
PANTHER:PTHR23253:SF32	SMART:SM00543	SMART:SM00544	SUPFAM:SSF48371	UniParc:UPI00023BD6E7	SEG:seg

Description

hypothetical protein

Coordinates

chr2:-:39045905..39052694

Molecular Weight (calculated)

86195.4 Da

IEP (calculated)

8.129

GRAVY (calculated)

-0.412

Length

784 amino acids

Sequence
(BLAST)

001: MQQSDQTVLS LRPGGGRGSR LLAPRFDSSS SSASPAFGSF PADLPLLRPH GGAPSPFSIK AGDARFEGRE RVRYTREQLL LLREGVEILD DVLKIKQDIE
101: AELFGEDQSW ARSENNPSQQ FRYSEPDNRD WRGRSGQLSG NADERSWDNL KENREFGNTN NRQDQLNSQF ARAQISSNQG GGPTPTLVKA EVPWSARRGT
201: LSDKDRVLKT VKGILNKLTP EKFDLLKGQL IDSGITSADI LKGVISLIFD KAVLEPTFCP MYAQLCSDLN EKLPPFPSDE PGGKEITFKR VLLNICQEAF
301: EGADKLRGEL RQMNAPDQEM ERRDKERLLK IRTLGNIRLI GELLKQKMVP EKIVHHIVQE LLGPPDIKVC PAEENVEAIC QFFNTIGKQL DESPKSRRIN
401: DMYFIRLKEL STNPQLAPRL RFMVRDVLDL RSNNWIPRRE EVKAKTITEI HSEAEKNLGL RPGATASMRS NRVVSGAQGN TGPGGFPIAR PGTGGLMPGM
501: PGTRRMPGMP GIDNDNWEMP RTRSMPRGDI SGMPTGGRGQ SPFLSKTSTV NSKLLPQGSG GIISGRSSAL VHGAGAPVRS PNLGFSAEPT PQIPSPVKAV
601: SAIPSEKPQP PAAKLNIDEL QRKTVSLLEE YFSVRLLDEA LQCVEELKAP AYYPEFVKEA ISLALDKSPP CVEPVANLFE YLLIKKILSA RDIGTGCMLF
701: ASLLDDIGID LPKAPNNFGE IIGKLVLAGG LDFKVVVEIL KKVEDDRFQK AIFSSALQVI SSVSGQAVLD SQASDIEACQ SLFN

Best Arabidopsis Sequence Match ( AT5G57870.2 )
(BLAST)

001: MQQGDQTVLS LRPGGGRGNR LFGSSSSSSS LSFGSLSSSD LPLLRPHGGA PASSFPFKGG DSRFDGRERV KYTREQLLEL KETTQLSDEI LRVQRETAAE
101: LFGEEGTWAR GESVVPVQSA SRFSEPDSRD WRSRSTQPPP SGEERSWDNL REAKDSRYVE ASQYNRQDQP NSQFSRANIS SNQGGGPAPV LVKAEVPWSA
201: RRGNLSENDR VLKTVKGILN KLTPEKYDLL KGQLIESGIT SADILKGVIT LIFDKAVLEP TFCPMYAKLC SDINDQLPTF PPAEPGDKEI TFKRVLLNIC
301: QEAFEGASQL REELRQMSAP DQEAERNDKE KLLKLKTLGN IRLIGELLKQ KMVPEKIVHH IVQELLGADE KVCPAEENVE AICHFFKTIG KQLDGNVKSK
401: RINDVYFKRL QALSKNPQLE LRLRFMVQNI IDMRSNGWVP RREEMKARTI TEIHTEAEKN LGLRPGATAN MRRGMVSSGG PVSPGPVYPG GRPGAGGLMP
501: GMPGTRRMPG MPGVDNDNWE VPRTRSMSRR DGPGPLHSPA VSKSASMNTR LLPQGSSGIM SGKTSALLQG SGSVSRPVTV SAERPAQSVA PLTVPVPVEK
601: PQPSGPKLSE EVLQRKTKSL LEEYFNVRLL GEALQCVEEL GLPSYHPEFV KEAISLSLEK SPPVVEPIAT LLEYLLSKKV VAPKDLETGF LLYGAMLDDI
701: GIDLPKAPNN FGEIVGKLIL AGGVDFKLVR EIIGKMEDDR FQKMVVDAAV RIVESSEQGK SLLASQAADI EACRNL

Arabidopsis Description

EIF(ISO)4G1Eukaryotic translation initiation factor isoform 4G-1 [Source:UniProtKB/Swiss-Prot;Acc:Q93ZT6]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.