Factsheet for Bra008045.1-P

Subcellular Localization

min:

: max

Winner_takes_all: extracellular, endoplasmic reticulum, vacuole, cytosol, plasma membrane, golgi

Predictor Summary:

nucleus 3
cytosol 2
extracellular 3
endoplasmic reticulum 3
vacuole 3
plasma membrane 3
golgi 3
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, secretory
Any Predictor:	cytosol, nucleus, plastid, secretory
BaCelLo:	nucleus
EpiLoc:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra020407.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G72730.1	Bra020407.1-P	AT5G57870.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY49861	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	100.0	100.0
AT1G72730.1	Thale cress	cytosol	92.97	95.89
Bra030892.1-P	Field mustard	cytosol	14.75	92.65
Bra030893.1-P	Field mustard	cytosol	88.52	91.75
Bra014365.1-P	Field mustard	cytosol	88.06	91.26
Bra001530.1-P	Field mustard	cytosol	88.29	90.62
Bra027387.1-P	Field mustard	cytosol	87.35	90.53
Bra021517.1-P	Field mustard	cytosol	87.35	90.53
KRH61534	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	87.35	90.31
KRH52210	Soybean	cytosol, endoplasmic reticulum	86.65	89.59
Bra037977.1-P	Field mustard	cytosol	85.48	88.59
Bra029124.1-P	Field mustard	cytosol	84.78	88.29
Solyc07g040750.1.1	Tomato	cytosol	84.31	87.17
Bra032127.1-P	Field mustard	cytosol	57.38	60.05
Bra022449.1-P	Field mustard	cytosol	57.38	60.05
Bra038231.1-P	Field mustard	cytosol	57.14	59.8
Bra016037.1-P	Field mustard	cytosol	96.02	56.4
Bra014267.1-P	Field mustard	cytosol	51.99	54.95
TraesCS6B01G217800.1	Wheat	cytosol	52.22	52.97
GSMUA_Achr9P13950_001	Banana	mitochondrion	34.66	48.21
TraesCS6A01G186600.1	Wheat	cytosol	53.86	41.82
Bra008527.1-P	Field mustard	cytosol	29.27	37.88
HORVU7Hr1G073770.1	Barley	cytosol, nucleus	52.69	35.16
Bra023311.1-P	Field mustard	cytosol	34.19	34.19
Bra023312.1-P	Field mustard	cytosol	33.96	33.26
Bra007587.1-P	Field mustard	cytosol	35.6	32.14
Bra008984.1-P	Field mustard	cytosol	31.85	31.92
Bra003442.1-P	Field mustard	cytosol	35.6	31.87
Bra037351.1-P	Field mustard	cytosol	35.6	29.8
Bra008983.1-P	Field mustard	cytosol	32.32	29.24
Bra000965.1-P	Field mustard	cytosol	34.89	29.04
Bra039312.1-P	Field mustard	cytosol	35.13	29.01
Bra040687.1-P	Field mustard	cytosol	32.79	28.23
Bra028547.1-P	Field mustard	cytosol	31.85	27.7
OQU84655	Sorghum	cytosol	48.01	26.8
Bra006091.1-P	Field mustard	cytosol	32.32	26.34
Os02t0221300-01	Rice	cytosol	52.22	25.31
Zm00001d016350_P001	Maize	vacuole	27.17	23.02
Bra023712.1-P	Field mustard	plastid	25.06	22.38
Zm00001d016351_P002	Maize	nucleus	45.2	21.64
KXG29825	Sorghum	cytosol	43.79	21.2
TraesCS6A01G186800.1	Wheat	cytosol	54.8	18.37
TraesCS6D01G173700.3	Wheat	cytosol	55.04	17.49
TraesCS6B01G217700.1	Wheat	cytosol	53.63	17.15

Protein Annotations

MapMan:17.4.2.1	Gene3D:3.40.50.300	EnsemblPlantsGene:Bra008045	EnsemblPlants:Bra008045.1	EnsemblPlants:Bra008045.1-P	InterPro:DEAD/DEAH_box_helicase_dom
GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003824	GO:GO:0004386	GO:GO:0005488
GO:GO:0005524	GO:GO:0016787	InterPro:Helicase_ATP-bd	InterPro:Helicase_C	InterPro:IPR001650	InterPro:IPR014001
InterPro:IPR014014	UniProt:M4CV00	InterPro:P-loop_NTPase	PFAM:PF00270	PFAM:PF00271	ScanProsite:PS00039
PFscan:PS51192	PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF551	InterPro:RNA-helicase_DEAD-box_CS
InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487	SMART:SM00490	SUPFAM:SSF52540	SignalP:SignalP-noTM	UniParc:UPI00025471BD
SEG:seg	:	:	:	:	:

Description

AT1G72730 (E=2e-225) | eukaryotic translation initiation factor 4A, putative / eIF-4A, putative

Coordinates

chrA02:-:12824024..12825964

Molecular Weight (calculated)

48507.5 Da

IEP (calculated)

5.110

GRAVY (calculated)

-0.178

Length

427 amino acids

Sequence
(BLAST)

001: MSFWFVFMYI SPVMAGMASE GTQYDPRQFD SKMNAILGEE GEETFYTTYD EVCDSFDAME LKSDLLRGIY AYGFEKPSAI QQRGIIPFCK GLDVIQQAQS
101: GTGKTATFCS GVLQQLDYTL VQCQALVLAP TRELAQQIEK VMRALGDYLG VKVHACVGGT SVREDQRILQ SGVHVVVGTP GRVFDMLRRQ SLRADAMKMF
201: VLDEADEMLS RGFKDQIYDI FQLLPSKVQV GVFSATMPPE ALEITRKFMN KPVRILVKRD ELTLEGIKQF YVNVDKEEWK LETLCDLYET LAITQSVIFV
301: NTRRKVDWLT DKMRSRDHTV SATHGDMDQN TRDIIMREFR SGSSRVLITT DLLARGIDVQ QVSLVINFDL PTQPENYLHR IGRSGRFGRK GVAINFMTTD
401: DERMLSDIQK FYNVVVEELP SNVADLL

Best Arabidopsis Sequence Match ( AT1G72730.1 )
(BLAST)

001: MAGMASDGTQ YDPRQFDTKM NAILGEEGEE TFYTNYDEVC DSFDAMELQP DLLRGIYAYG FEKPSAIQQR GIIPFCKGLD VIQQAQSGTG KTATFCSGVL
101: QQLDISLVQC QALVLAPTRE LAQQIEKVMR ALGDYLGVKA QACVGGTSVR EDQRVLQSGV HVVVGTPGRV FDLLRRQSLR ADAIKMFVLD EADEMLSRGF
201: KDQIYDIFQL LPSKVQVGVF SATMPPEALE ITRKFMNKPV RILVKRDELT LEGIKQFYVN VDKEEWKLET LCDLYETLAI TQSVIFVNTR RKVDWLTDKM
301: RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS LVINFDLPTQ PENYLHRIGR SGRFGRKGVA INFMTSEDER MMADIQRFYN
401: VVVEELPSNV ADLL

Arabidopsis Description

TIF4A-3Eukaryotic initiation factor 4A-3 [Source:UniProtKB/Swiss-Prot;Acc:Q9CAI7]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.