Factsheet for Os02t0221300-01

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 4
cytosol 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
EpiLoc:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0611500-01
Os04t0499300-01
Os06t0520600-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G72730.1	Os02t0611500-01	AT5G57870.1	21798944
AT1G72730.1	Os04t0499300-01	AT5G57870.1	21798944
AT1G72730.1	Os06t0520600-01	AT5G57870.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS6B01G217800.1	Wheat	cytosol	29.74	62.23
VIT_04s0008g00980.t01	Wine grape	cytosol	5.68	59.52
Solyc12g096000.1.1	Tomato	cytosol, nucleus, unclear	25.88	57.87
Os02t0146600-01	Rice	plastid	26.9	57.25
Os06t0701100-02	Rice	extracellular, nucleus, plastid	26.79	57.0
Solyc12g095990.1.1	Tomato	nucleus	26.56	56.66
KRH61534	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	26.56	56.66
Solyc07g040750.1.1	Tomato	cytosol	26.45	56.42
KRH52210	Soybean	cytosol, endoplasmic reticulum	26.45	56.42
PGSC0003DMT400075336	Potato	cytosol	26.22	56.34
Solyc08g062800.2.1	Tomato	extracellular, plastid	26.33	56.17
PGSC0003DMT400075339	Potato	cytosol	26.33	56.17
PGSC0003DMT400021166	Potato	cytosol	26.33	56.17
Bra021517.1-P	Field mustard	cytosol	26.22	56.07
CDY39079	Canola	cytosol	26.22	56.07
Bra027387.1-P	Field mustard	cytosol	26.11	55.83
CDY02469	Canola	cytosol	26.11	55.83
CDY10607	Canola	cytosol	25.99	55.72
Bra030893.1-P	Field mustard	cytosol	25.99	55.58
AT1G54270.1	Thale cress	cytosol	25.99	55.58
Bra001530.1-P	Field mustard	cytosol	26.11	55.29
VIT_04s0008g00990.t01	Wine grape	cytosol	7.72	55.28
TraesCS6A01G186600.1	Wheat	cytosol	34.51	55.27
CDY08220	Canola	cytosol	25.77	55.1
Bra014365.1-P	Field mustard	cytosol	25.65	54.85
Bra029124.1-P	Field mustard	cytosol	24.97	53.66
Bra037977.1-P	Field mustard	cytosol	25.09	53.64
CDY24057	Canola	cytosol	24.97	53.4
AT1G72730.1	Thale cress	cytosol	25.09	53.38
CDY33073	Canola	cytosol	24.74	52.91
CDY32586	Canola	cytosol	22.7	52.91
CDY49861	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	25.31	52.22
Bra008045.1-P	Field mustard	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	25.31	52.22
CDY55205	Canola	cytosol	24.74	51.54
CDX97528	Canola	cytosol	24.86	51.53
CDX82505	Canola	cytosol	24.97	51.4
AT3G13920.5	Thale cress	cytosol	24.4	51.31
CDX75739	Canola	cytosol	25.09	50.69
CDY67069	Canola	cytosol	5.79	50.49
CDY72259	Canola	cytosol	12.71	50.45
OQU84655	Sorghum	cytosol	42.79	49.28
HORVU7Hr1G073770.1	Barley	cytosol, nucleus	35.75	49.22
CDY11154	Canola	cytosol	10.44	45.54
Zm00001d016351_P002	Maize	nucleus	43.47	42.94
Os03t0566800-01	Rice	cytosol, nucleus, plasma membrane	19.64	42.82
Os01t0639100-01	Rice	nucleus	19.64	42.82
KXG29825	Sorghum	cytosol	42.22	42.18
CDY54037	Canola	cytosol	12.71	41.95
TraesCS6A01G186800.1	Wheat	cytosol	56.07	38.78
GSMUA_Achr9P13950_001	Banana	mitochondrion	13.51	38.76
CDY53867	Canola	cytosol	18.16	37.91
TraesCS6D01G173700.3	Wheat	cytosol	56.98	37.35
TraesCS6B01G217700.1	Wheat	cytosol	55.39	36.55
Zm00001d016350_P001	Maize	vacuole	19.75	34.52
CDX72939	Canola	mitochondrion, nucleus	18.16	34.33
Bra016037.1-P	Field mustard	cytosol	27.36	33.15
Os01t0549700-01	Rice	cytosol, plastid	14.19	28.94
Os01t0550000-01	Rice	cytosol, nucleus, plasma membrane	14.07	28.7
Os04t0533000-02	Rice	cytosol	14.87	26.31
Os02t0641800-02	Rice	plasma membrane, plastid	14.76	25.59
Os10t0503700-01	Rice	plasma membrane	14.98	25.34
CDY68148	Canola	nucleus	20.43	24.36
Os03t0158200-01	Rice	cytosol, plasma membrane, plastid	12.49	21.78
Os01t0970600-01	Rice	plastid	8.97	16.67
Os03t0219700-01	Rice	plastid	10.67	16.4
Os02t0636300-01	Rice	nucleus	10.44	16.06
CDY49860	Canola	cytosol	1.25	8.8

Protein Annotations

Gene3D:3.40.50.300	MapMan:35.1	EntrezGene:4328756	UniProt:A0A0N7KEY4	ProteinID:BAS77705.1	InterPro:DEAD/DEAH_box_helicase_dom
InterPro:DUF4283	GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0003743
GO:GO:0003824	GO:GO:0004004	GO:GO:0004386	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005730	GO:GO:0005737	GO:GO:0006139
GO:GO:0006412	GO:GO:0006413	GO:GO:0008135	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058
GO:GO:0009987	GO:GO:0010501	GO:GO:0016787	GO:GO:0019538	InterPro:Helicase_ATP-bd	InterPro:Helicase_C
InterPro:IPR001650	InterPro:IPR014001	InterPro:IPR014014	EnsemblPlantsGene:Os02g0221300	EnsemblPlants:Os02t0221300-01	InterPro:P-loop_NTPase
PFAM:PF00270	PFAM:PF00271	PFAM:PF14111	ScanProsite:PS00039	PFscan:PS51192	PFscan:PS51194
PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF534	InterPro:RNA-helicase_DEAD-box_CS	InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487
SMART:SM00490	SUPFAM:SSF52540	UniParc:UPI0001C7DC8D	RefSeq:XP_015622546.1	RefSeq:XP_015622547.1	:

Description

RNA helicase 19Similar to cDNA clone:001-114-H08, full insert sequence. (Os02t0221300-01)

Coordinates

chr2:+:6771313..6778380

Molecular Weight (calculated)

98481.8 Da

IEP (calculated)

5.674

GRAVY (calculated)

-0.404

Length

881 amino acids

Sequence
(BLAST)

001: MAQQEHNMVT KGTERLVAGA QLFGHSTRQP FYMIQPSEEV VQKEKFYYHC LLITSDVSNL DLRKVKVEVS KFWKLTDWEI RTEGRHNFLV SLNSEDDLVS
101: CLKIPRIETS IDDKEVNFTV ARWKEDDDEN IELIREWILV YGVPRIYRNW KELYQIASAV GVLIDVDEES LEGDHKEPIK LKVALRGVDA SPFSCHFVFG
201: WYSRCVTFTI EDKAQTTECE REVLEDCNGE DHPDEFGKEY NNKTDKLIEM PPEILNKGMN LESSSHDTSI VVSTHNHTLD VKGCSDKELE AIESAVLVEE
301: PSSIEDNNIS APVPTTTIIS EKTAEDSPRA GREQSISGSS TSMIGDTLFK ATVIHSKKTT EDIPKAEGGQ STSGNSTSII GEEPFRGIQK PPVKIVYARR
401: NKRSMEKQDY KSFLEKQHQK EMEAAIVGQR QKSDDESGGD GNNKDSNSIA PSAIAINSKK KQTTKDIVTT QGAQFISESL IGETQTKGTG GAQFNANQHI
501: SKMSKQEHNE IFKSFNEMGL QESLLKGIYA YDLDKPSAVH QRGIVPLCNG LDIIQQSLFG TTVTLCCGIL QRLDYASTEC QALVLVPTHD LAHETQNVIG
601: VLGQFLSAKA HAFCGGTSAH EDQQILSTGV QVAVGTPCHV LGMLQGRALC PDHIRMFVLD EADEVLRGFK DQIHGIIQFL PTKTQFGFFS ASMSHEALEM
701: CRKYMNKPVE IIVPRDEELE GINVKQFYVN VEKEDCKLDK LCGLFDTMEI TRSIIFVNTR HHAKSLTEKI RGKGYTVSAI HGGIHQRARD KAVQEFQSGS
801: SRILITTDLR GIDVLRAPAA IFYDLPTQPV CYLRHVQSGQ HGRKGVAISF ITSTDERVFS TIQKFCNTQI EELPSNVADL L

Best Arabidopsis Sequence Match ( AT1G54270.1 )
(BLAST)

001: MAGSAPEGTQ FDTRQFDQRL NEVLDGQDEF FTSYDEVHES FDAMGLQENL LRGIYAYGFE KPSAIQQRGI VPFCKGLDVI QQAQSGTGKT ATFCSGVLQQ
101: LDYALLQCQA LVLAPTRELA QQIEKVMRAL GDYQGVKVHA CVGGTSVRED QRILQAGVHV VVGTPGRVFD MLRRQSLRPD CIKMFVLDEA DEMLSRGFKD
201: QIYDIFQLLP PKIQVGVFSA TMPPEALEIT RKFMSKPVRI LVKRDELTLE GIKQFYVNVE KEDWKLETLC DLYETLAITQ SVIFVNTRRK VDWLTDKMRS
301: RDHTVSATHG DMDQNTRDII MREFRSGSSR VLITTDLLAR GIDVQQVSLV INFDLPTQPE NYLHRIGRSG RFGRKGVAIN FVTLDDQRML FDIQKFYNVV
401: VEELPSNVAD LL

Arabidopsis Description

TIF4A-2eIF4A-2 [Source:UniProtKB/TrEMBL;Acc:A0A178W4U1]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.