Factsheet for Os02t0641800-02

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, plastid

Predictor Summary:

nucleus 4
plastid 1
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704
plastid: 27992503

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0111200-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G45810.1	Os01t0111200-01	AT1G26110.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Os04t0533000-02	Rice	cytosol	90.35	92.17
KRH04755	Soybean	cytosol	47.64	91.32
CDY54061	Canola	cytosol	57.09	88.96
VIT_02s0025g03790.t01	Wine grape	cytosol, plastid	82.68	87.5
CDY45702	Canola	cytosol	35.24	86.89
Bra008527.1-P	Field mustard	cytosol	55.51	85.45
Solyc10g017530.2.1	Tomato	nucleus	82.09	84.93
KRH65098	Soybean	endoplasmic reticulum, nucleus	82.87	83.86
KRH48170	Soybean	endoplasmic reticulum	82.87	83.7
KRH48173	Soybean	mitochondrion	82.28	83.6
KRH65096	Soybean	nucleus	82.09	83.57
Solyc01g094350.2.1	Tomato	nucleus	80.51	82.13
AT4G00660.1	Thale cress	cytosol, mitochondrion	81.3	81.78
CDY07205	Canola	cytosol	78.94	81.67
CDY51147	Canola	cytosol	79.72	81.65
CDX91901	Canola	cytosol	80.12	81.24
Os10t0503700-01	Rice	plasma membrane	82.87	80.81
Bra037351.1-P	Field mustard	cytosol	80.91	80.59
CDX74342	Canola	cytosol	80.51	79.57
Bra000965.1-P	Field mustard	cytosol	80.31	79.53
Bra007587.1-P	Field mustard	cytosol	72.83	78.22
Bra003442.1-P	Field mustard	cytosol	73.23	77.99
CDY58787	Canola	cytosol	73.82	77.8
AT3G61240.1	Thale cress	cytosol	75.98	77.51
CDX71756	Canola	cytosol	72.64	77.04
CDY48935	Canola	cytosol, plastid	71.85	76.84
CDY37067	Canola	cytosol	76.57	76.27
CDY27357	Canola	cytosol, plastid	75.59	75.44
Bra039312.1-P	Field mustard	cytosol	76.57	75.24
GSMUA_Achr11P...	Banana	cytosol	83.46	75.04
AT2G45810.1	Thale cress	cytosol, plastid	76.97	74.05
Solyc05g048850.2.1	Tomato	mitochondrion	72.83	68.9
Os02t0146600-01	Rice	plastid	30.12	36.96
Os06t0701100-02	Rice	extracellular, nucleus, plastid	30.12	36.96
Os03t0566800-01	Rice	cytosol, nucleus, plasma membrane	28.94	36.39
Os01t0639100-01	Rice	nucleus	28.94	36.39
Os01t0550000-01	Rice	cytosol, nucleus, plasma membrane	28.74	33.8
Os01t0549700-01	Rice	cytosol, plastid	28.74	33.8
Os03t0158200-01	Rice	cytosol, plasma membrane, plastid	24.21	24.36
Os01t0970600-01	Rice	plastid	19.29	20.68
Os03t0219700-01	Rice	plastid	21.06	18.67
Os02t0636300-01	Rice	nucleus	20.47	18.15
Os02t0221300-01	Rice	cytosol	25.59	14.76

Protein Annotations

Gene3D:3.40.50.300	MapMan:35.1	EntrezGene:4330120	EMBL:AK066504	EMBL:AK072124	ProteinID:BAD25226.1
ProteinID:BAD25227.1	ProteinID:BAF09466.1	ProteinID:BAS79992.1	ProteinID:BAS79993.1	InterPro:DEAD/DEAH_box_helicase_dom	ProteinID:EEE57460.1
GO:GO:0000166	GO:GO:0000932	GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0003824
GO:GO:0004004	GO:GO:0004386	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005634	GO:GO:0005730	GO:GO:0005737	GO:GO:0006139	GO:GO:0006397
GO:GO:0006412	GO:GO:0006417	GO:GO:0006810	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058
GO:GO:0009987	GO:GO:0010494	GO:GO:0010501	GO:GO:0016043	GO:GO:0016787	GO:GO:0019538
GO:GO:0033962	GO:GO:0051028	InterPro:Helicase_ATP-bd	InterPro:Helicase_C	InterPro:IPR001650	InterPro:IPR014001
InterPro:IPR014014	EnsemblPlantsGene:Os02g0641800	EnsemblPlants:Os02t0641800-02	InterPro:P-loop_NTPase	PFAM:PF00270	PFAM:PF00271
ScanProsite:PS00039	PFscan:PS51192	PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF352
UniProt:Q6H7S2	InterPro:RNA-helicase_DEAD-box_CS	InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487	SMART:SM00490	SUPFAM:SSF52540
UniParc:UPI00003B43CA	RefSeq:XP_015627069.1	SEG:seg	:	:	:

Description

RNA helicase 8Similar to RNA helicase (Fragment). (Os02t0641800-01);Similar to ATP-dependent RNA helicase dhh1. (Os02t0641800-02)

Coordinates

chr2:+:25767387..25774435

Molecular Weight (calculated)

58169.9 Da

IEP (calculated)

8.760

GRAVY (calculated)

-0.512

Length

508 amino acids

Sequence
(BLAST)

001: MDPRARYPPG IGNGRGGNPN YYNRGPPLQQ QHNHHQQQQT SAPHHQQYVQ RQPQQHHHHN HHQQHQQQQQ QWLRRNQIAR EAAGTDRNSE PKAVAQSPAV
101: DGIDSSSQDW KAQLKLPPQD TRYRTEDVTA TKGNEFEDYF LKRELLMGIY EKGFERPSPI QEESIPIALT GSDILARAKN GTGKTAAFCI PALEKIDQEK
201: NAIQVVILVP TRELALQTSQ VCKELGKHLK IQVMVTTGGT SLKDDIIRLY QPVHLLVGTP GRILDLTKKG ICILKDCSML IMDEADKLLS PEFQPSVEQL
301: IRYLPASRQI LMFSATFPVT VKEFKDKYLP KPYVINLMDE LTLKGITQFY AFVEERQKVH CLNTLFSKLQ INQSIIFCNS VNRVELLAKK ITELGYSCFY
401: IHAKMLQDHR NRVFHDFRNG ACRNLVCTDL FTRGIDIQAV NVVINFDFPK TAETYLHRVG RSGRFGHLGL AVNLITYEDR FNLYRIEQEL GTEIKPIPPQ
501: IDQAIYCQ

Best Arabidopsis Sequence Match ( AT4G00660.2 )
(BLAST)

001: MNNRGRYPPG IGAGRGAFNP NPNYQSRSGY QQHPPPQYVQ RGNYAQNHQQ QFQQAPSQPH QYQQQQQQQQ QWLRRGQIPG GNSNGDAVVE VEKTVQSEVI
101: DPNSEDWKAR LKLPAPDTRY RTEDVTATKG NEFEDYFLKR ELLMGIYEKG FERPSPIQEE SIPIALTGRD ILARAKNGTG KTAAFCIPVL EKIDQDNNVI
201: QAVIIVPTRE LALQTSQVCK ELGKHLKIQV MVTTGGTSLK DDIMRLYQPV HLLVGTPGRI LDLTKKGVCV LKDCSVLVMD EADKLLSQEF QPSVEHLISF
301: LPESRQILMF SATFPVTVKD FKDRFLTNPY VINLMDELTL KGITQFYAFV EERQKIHCLN TLFSKLQINQ SIIFCNSVNR VELLAKKITE LGYSCFYIHA
401: KMLQDHRNRV FHDFRNGACR NLVCTDLFTR GIDIQAVNVV INFDFPKNAE TYLHRVGRSG RFGHLGLAVN LITYEDRFNL YRIEQELGTE IKQIPPHIDQ
501: AIYCQ

Arabidopsis Description

RH8DEAD-box ATP-dependent RNA helicase 8 [Source:UniProtKB/Swiss-Prot;Acc:Q8RXK6]
SUBAcon: [mitochondrion,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.