CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 2
mitochondrion 4
nucleus 1
extracellular 1
endoplasmic reticulum 1
vacuole 2
plasma membrane 1
golgi 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	mitochondrion, nucleus, plastid, secretory
BaCelLo:	plastid
iPSORT:	mitochondrion
MultiLoc:	mitochondrion
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	mitochondrion
WoLF PSORT:	plastid
YLoc:	vacuole

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH13813	Soybean	nucleus	94.31	94.31
VIT_02s0025g04190.t01	Wine grape	endoplasmic reticulum, vacuole	67.97	68.58
Solyc08g080550.1.1	Tomato	nucleus	63.52	65.15
PGSC0003DMT400007864	Potato	golgi, mitochondrion, plastid	63.52	65.15
AT1G12770.2	Thale cress	mitochondrion, plastid	58.72	59.89
Os02t0636300-01	Rice	nucleus	60.32	59.16
Os03t0219700-01	Rice	plastid	59.96	58.81
OQU85369	Sorghum	mitochondrion, plastid	59.43	58.29
HORVU6Hr1G059910.1	Barley	mitochondrion	59.43	55.57
TraesCS6A01G234000.1	Wheat	mitochondrion	59.25	55.41
TraesCS6B01G262800.1	Wheat	mitochondrion	58.9	55.07
TraesCS6D01G216300.1	Wheat	mitochondrion	52.31	54.65
GSMUA_Achr1P01890_001	Banana	plastid	51.25	54.44
Zm00001d017345_P002	Maize	plastid	59.61	52.76
KRH12197	Soybean	nucleus	20.28	28.08
KRH37217	Soybean	endoplasmic reticulum	20.28	28.01
KRH37426	Soybean	cytosol, endoplasmic reticulum	20.46	27.84
KRH12430	Soybean	cytosol	20.46	27.84
KRH02682	Soybean	cytosol, endoplasmic reticulum, nucleus	20.28	27.6
KRH19215	Soybean	cytosol, endoplasmic reticulum, nucleus	20.28	27.6
KRH52210	Soybean	cytosol, endoplasmic reticulum	20.11	27.36
KRH61534	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	20.11	27.36
KRH47074	Soybean	nucleus	19.93	27.12
KRH10048	Soybean	cytosol, endoplasmic reticulum	19.93	27.12
KRH23270	Soybean	cytosol	19.93	27.12
KRH44102	Soybean	nucleus	19.93	26.6
KRH44441	Soybean	cytosol, nucleus	19.75	26.06
KRH47464	Soybean	nucleus	19.75	26.06
KRH04755	Soybean	cytosol	12.28	26.04
KRH61783	Soybean	cytosol	19.57	25.76
KRH52452	Soybean	nucleus	19.57	25.76
KRH51049	Soybean	plastid	20.64	24.58
KRG99590	Soybean	endoplasmic reticulum, nucleus	21.17	24.39
KRG93607	Soybean	nucleus	21.35	24.24
KRH02098	Soybean	cytosol	9.43	23.04
KRH48173	Soybean	mitochondrion	19.04	21.4
KRH65098	Soybean	endoplasmic reticulum, nucleus	19.04	21.31
KRH48170	Soybean	endoplasmic reticulum	19.04	21.27
KRH65096	Soybean	nucleus	18.86	21.24
KRH40488	Soybean	cytosol	18.68	19.48

Protein Annotations

EntrezGene:100790663	Gene3D:3.40.50.300	MapMan:35.1	EMBL:ACUP02004873	InterPro:DEAD/DEAH_box_helicase_dom	EnsemblPlantsGene:GLYMA_08G162000
GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003824	GO:GO:0004004	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005730
GO:GO:0005737	GO:GO:0006139	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0010501
GO:GO:0016787	InterPro:Helicase_ATP-bd	InterPro:Helicase_C	UniProt:I1KTU7	InterPro:IPR001650	InterPro:IPR014001
InterPro:IPR014014	EnsemblPlants:KRH43643	ProteinID:KRH43643	ProteinID:KRH43643.1	InterPro:P-loop_NTPase	PFAM:PF00270
PFAM:PF00271	PFscan:PS51192	PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF537
InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487	SMART:SM00490	SUPFAM:SSF52540	UniParc:UPI00023383E4	SEG:seg

Description

hypothetical protein

Coordinates

chr8:+:12590289..12592554

Molecular Weight (calculated)

61386.5 Da

IEP (calculated)

9.898

GRAVY (calculated)

-0.201

Length

562 amino acids

Sequence
(BLAST)

001: MPTLITSRFL LLVGESLHMR RGLATSRSVL LHTSSVQCMS QVEPHHGSVT LSSIGLQTET EPRNRSKTNK VKPLGSPSEN LKSKGKPFGI NEKKKTFRVV
101: EKQQIESAPF AANSFSELGL PLVLIERLEK EGFTVPTEVQ SAAVPTILNN RDVIIQSYTG SGKTLAYLLP ILSVVGPLRG EIGEGDSDGG ECGKKLGIEA
201: VIVAPSRELG MQIVREFEKV LGMDNKRAVQ QLVGGANRTR QEDALKKNKP AIVVGTPGRI AELSASGKLR THSCRFLVLD EVDELLSFNF REDMHRILEH
301: VGRRSGADQN SDSRKAERQL IMVSATVPFS VVRAARSWGC DPLLVQAKKV APLGTVSPSE PISLSQSSPS SSPSLAMPSP AAVESLPPAL KHYYFVTRVQ
401: HKVDVLRRCI HALDAKFVIA FMNHTKQLKD VVFKLEARGM KAMELHGDLG KLARSTTLKK FKNGEVRVLV TNELSARGLD VAECDLVVNL DLPTDSIHYA
501: HRAGRTGRLG RNGTVVTICE ESEVFVVKKL QKQLGIPIAS CDFAEGKLLV SEEEKTVSTS ER

Best Arabidopsis Sequence Match ( AT1G12770.1 )
(BLAST)

001: MAASTSTRFL VLLKDFSAFR KISWTCAATN FHRQSRFLCH VAKEDGSLTL ASLDLGNKPR KFGKGKAMKL EGSFVTEMGQ GKVRAVKNDK MKVVKEKKPA
101: EIVSPLFSAK SFEELGLPDS LLDSLEREGF SVPTDVQSAA VPAIIKGHDA VIQSYTGSGK TLAYLLPILS EIGPLAEKSR SSHSENDKRT EIQAMIVAPS
201: RELGMQIVRE VEKLLGPVHR RMVQQLVGGA NRMRQEEALK KNKPAIVVGT PGRIAEISKG GKLHTHGCRF LVLDEVDELL SFNFREDIHR ILEHVGKRSG
301: AGPKGEVDER ANRQTILVSA TVPFSVIRAA KSWSHEPVLV QANKVTPLDT VQPSAPVMSL TPTTSEADGQ IQTTIQSLPP ALKHYYCISK HQHKVDTLRR
401: CVHALDAQSV IAFMNHSRQL KDVVYKLEAR GMNSAEMHGD LGKLGRSTVL KKFKNGEIKV LVTNELSARG LDVAECDLVV NLELPTDAVH YAHRAGRTGR
501: LGRKGTVVTV CEESQVFIVK KMEKQLGLPF LYCEFVDGEL VVTEEDKAII R

Arabidopsis Description

RH47DEAD-box ATP-dependent RNA helicase 47, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q8W4E1]
SUBAcon: [mitochondrion,plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.