CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 1
mitochondrion 5
plasma membrane 1
peroxisome 1
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG98502
KRG98507
KRH46360
KRH46364

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G55940.1	KRG98502	AT4G26630.1	25387881
AT3G55940.1	KRG98507	AT4G26630.1	25387881
AT3G55940.1	KRH46360	AT4G26630.1	25387881
AT3G55940.1	KRH46364	AT4G26630.1	25387881

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH73190	Soybean	endoplasmic reticulum	97.33	97.33
KRH31148	Soybean	mitochondrion	76.5	77.53
KRG97732	Soybean	mitochondrion	76.0	77.03
VIT_13s0067g00900.t01	Wine grape	mitochondrion	75.0	75.89
Bra029697.1-P	Field mustard	mitochondrion	68.67	73.05
Bra040049.1-P	Field mustard	cytosol	65.5	72.51
AT3G08510.1	Thale cress	mitochondrion	70.0	72.29
CDY27998	Canola	cytosol	65.33	71.79
CDY35983	Canola	mitochondrion	70.0	71.79
CDY22153	Canola	mitochondrion	69.17	71.43
Bra007201.1-P	Field mustard	mitochondrion	64.67	71.06
CDY70294	Canola	mitochondrion	64.17	70.51
Solyc05g052760.2.1	Tomato	plastid	68.33	70.33
PGSC0003DMT400069760	Potato	mitochondrion	68.0	69.74
PGSC0003DMT400016945	Potato	endoplasmic reticulum	57.17	69.43
AT3G55940.1	Thale cress	mitochondrion	67.33	69.18
CDY01144	Canola	cytosol	54.17	69.15
CDY27254	Canola	mitochondrion	66.83	68.9
CDX72188	Canola	mitochondrion	66.33	68.38
CDY47356	Canola	mitochondrion	63.5	67.55
Bra032722.1-P	Field mustard	mitochondrion	63.0	67.14
Solyc06g051620.2.1	Tomato	cytosol, mitochondrion	62.67	66.55
KRG97734	Soybean	cytosol	56.0	60.32
GSMUA_Achr10P...	Banana	mitochondrion	51.5	59.88
KRH14947	Soybean	cytosol, mitochondrion	59.17	59.56
KRH72676	Soybean	cytosol, mitochondrion	37.17	59.31
KRH73189	Soybean	cytosol	58.0	57.62
HORVU1Hr1G014570.19	Barley	mitochondrion	54.33	55.92
KRH17039	Soybean	cytosol, mitochondrion	53.67	55.9
TraesCS1A01G069300.1	Wheat	mitochondrion	54.33	55.73
TraesCS1D01G071800.3	Wheat	mitochondrion	54.83	55.57
KRH31146	Soybean	nucleus	55.67	54.75
HORVU1Hr1G014580.9	Barley	mitochondrion	54.67	53.77
Os05t0127200-01	Rice	plasma membrane	53.17	53.34
OQU77319	Sorghum	mitochondrion	53.33	53.16
KRH14948	Soybean	cytosol	51.0	50.75
KRH31144	Soybean	cytosol	48.83	50.26
KRH73188	Soybean	cytosol	49.0	49.49
KRG97735	Soybean	cytosol	50.5	48.71
Zm00001d047447_P002	Maize	extracellular	52.33	47.22
KRH14952	Soybean	cytosol	27.17	41.9

Protein Annotations

KEGG:00562+3.1.4.11	KEGG:04070+3.1.4.11	Gene3D:1.10.238.10	Gene3D:2.60.40.150	Gene3D:3.20.20.190	MapMan:5.7.2.3.2
EntrezGene:547864	EMBL:ACUP02008546	InterPro:C2_dom	InterPro:C2_domain_sf	InterPro:EF-hand-dom_pair	EnsemblPlantsGene:GLYMA_14G059200
GO:GO:0003674	GO:GO:0003824	GO:GO:0004435	GO:GO:0004871	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0006629	GO:GO:0007154	GO:GO:0007165	GO:GO:0008081	GO:GO:0008150
GO:GO:0008152	GO:GO:0009056	GO:GO:0009987	GO:GO:0016042	GO:GO:0016787	GO:GO:0035556
InterPro:IPR000008	InterPro:IPR000909	InterPro:IPR001711	InterPro:IPR017946	InterPro:IPR035892	EnsemblPlants:KRH14945
ProteinID:KRH14945	ProteinID:KRH14945.1	PFAM:PF00168	PFAM:PF00387	PFAM:PF00388	PFAM:PF09279
InterPro:PI-PLC_fam	InterPro:PLC-like_Pdiesterase_TIM-brl	InterPro:PLC_EF-hand-like	InterPro:PLipase_C_PInositol-sp_X_dom	InterPro:PLipase_C_Pinositol-sp_Y	PRINTS:PR00390
PFscan:PS50004	PFscan:PS50007	PFscan:PS50008	PANTHER:PTHR10336	PANTHER:PTHR10336:SF92	MetaCyc:PWY-6351
MetaCyc:PWY-6367	MetaCyc:PWY-7039	UniProt:Q43443	SMART:SM00148	SMART:SM00149	SMART:SM00239
SUPFAM:SSF47473	SUPFAM:SSF49562	SUPFAM:SSF51695	EMBL:U41474	UniParc:UPI00000ACC50	SEG:seg

Description

hypothetical protein

Coordinates

chr14:-:4790214..4795140

Molecular Weight (calculated)

68700.3 Da

IEP (calculated)

6.033

GRAVY (calculated)

-0.585

Length

600 amino acids

Sequence
(BLAST)

001: MTSKQTYSVC FCWRRRFKLA LAEAPSEIKT LFNEYSENEL MTPSHLKRFL VDVQRQEKAT EEDAQAIIDS FRHFHRRGAG LNLETFFKYL FSDDNPPLLP
101: SHGVHHDMTL PLSHYFIYTG HNSYLTGNQL SSDCSDVPII NALKKGVRVI ELDIWPNASK DSIDVLHGRT LTTPVELIRC LRSIKDHAFV ASEYPVVITL
201: EDHLTPDLQA KVAEMVTQTF GDILFTPNSE SVKEFPSPES LKKRIIISTK PPKEYLEAKE KEKGDDSQHE KEKGDDSEHG KASGEDEAWG KEVPSLKGGT
301: IEDYKDNNVD EDLNDEEEFD ESDKSHHNEA PEYRHLIAIH AGKPKGGLVE CLKVDPEKVR RLSLSEQQLE KAAINYGQQI VRFTQRNILR VYPKGTRIDS
401: SNYNPLIGWM HGAQMVAFNM QGYGRSLWLM HGMFRANGGC GYVKKPNFLL ETGPDDEVFN PKAKLPVKTT LKVTVYMGEG WYYDFKHTHF DQYSPPDFYT
501: RVGIAGVPND TIMKRTKAIE DNWLPTWNEA FEFPLTVPEL ALLRIEVHEY DMSEKDDFGG QTCLPIWELR SGIRAIPLHS QKGDKYNTVK LLMRFEFINN

Best Arabidopsis Sequence Match ( AT3G08510.1 )
(BLAST)

001: MSKQTYKVCF CFRRRFRYTA SEAPREIKTI FEKYSENGVM TVDHLHRFLI DVQKQDKATR EDAQSIINSA SSLLHRNGLH LDAFFKYLFG DNNPPLALHK
101: VHHDMDAPIS HYFIFTGHNS YLTGNQLSSD CSEVPIIDAL KKGVRVIELD IWPNSNKDDI DVLHGMTLTT PVGLIKCLKA IRAHAFDVSD YPVVVTLEDH
201: LTPDLQSKVA EMVTEIFGEI LFTPPVGESL KEFPSPNSLK RRIIISTKPP KEYKEGKDVE VVQKGKDLGD EEVWGREVPS FIQRNKSEAK DDLDGNDDDD
301: DDDDEDKSKI NAPPQYKHLI AIHAGKPKGG ITECLKVDPD KVRRLSLSEE QLEKAAEKYA KQIVRFTQHN LLRIYPKGTR VTSSNYNPLV GWSHGAQMVA
401: FNMQGYGRSL WLMQGMFRAN GGCGYIKKPD LLLKSGSDSD IFDPKATLPV KTTLRVTVYM GEGWYFDFRH THFDQYSPPD FYTRVGIAGV PGDTVMKKTK
501: TLEDNWIPAW DEVFEFPLTV PELALLRLEV HEYDMSEKDD FGGQTCLPVW ELSEGIRAFP LHSRKGEKYK SVKLLVKVEF V

Arabidopsis Description

PLC2Phosphoinositide phospholipase C [Source:UniProtKB/TrEMBL;Acc:A0A178VDK7]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.