CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

plastid 4
cytosol 3
nucleus 1
mitochondrion 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 23041469
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH03376
KRH54462
KRH57009
KRH63457

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G46430.1	KRH03376	AT3G48100.1	18642946
AT5G46430.1	KRH54462	AT3G48100.1	18642946
AT5G46430.1	KRH57009	AT3G48100.1	18642946
AT5G46430.1	KRH63457	AT3G48100.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH31095	Soybean	nucleus	99.25	99.25
KRH58920	Soybean	cytosol, plastid	99.25	99.25
KRH42824	Soybean	cytosol, plastid	99.25	99.25
KRH42825	Soybean	nucleus	97.74	97.74
KRH18320	Soybean	endoplasmic reticulum	95.49	95.49
KRH12802	Soybean	cytosol	94.74	94.74
VIT_10s0003g02650.t01	Wine grape	cytosol, plastid	93.98	93.98
GSMUA_Achr7P19740_001	Banana	cytosol	91.73	91.73
GSMUA_Achr3P02470_001	Banana	cytosol, mitochondrion, nucleus, plastid	90.23	90.23
GSMUA_Achr10P...	Banana	cytosol	90.23	90.23
Os09t0502000-01	Rice	nucleus	87.22	87.22
Os09t0501200-01	Rice	nucleus	87.22	87.22
TraesCS7B01G152300.1	Wheat	cytosol, mitochondrion	87.22	86.57
PGSC0003DMT400022579	Potato	cytosol	86.47	86.47
Os08t0524600-00	Rice	cytosol, endoplasmic reticulum	27.07	85.71
Solyc08g006900.2.1	Tomato	nucleus	85.71	85.71
TraesCS5A01G279100.1	Wheat	golgi	85.71	85.71
TraesCS7A01G255800.1	Wheat	cytosol, mitochondrion	85.71	85.71
TraesCS7B01G152900.1	Wheat	golgi, unclear	85.71	85.71
EER97002	Sorghum	cytosol	84.96	84.96
HORVU7Hr1G052800.8	Barley	cytosol	84.96	84.96
Zm00001d049790_P007	Maize	cytosol	84.21	84.21
Zm00001d021020_P001	Maize	mitochondrion, plastid	84.21	84.21
Zm00001d006064_P002	Maize	cytosol	84.21	83.58
EES15331	Sorghum	cytosol	83.46	83.46
EER99136	Sorghum	cytosol	83.46	83.46
TraesCS5D01G286400.1	Wheat	cytosol	83.46	83.46
TraesCS5B01G278700.1	Wheat	cytosol	83.46	83.46
TraesCS5A01G279400.1	Wheat	unclear	83.46	83.46
Solyc02g070310.2.1	Tomato	nucleus, plastid	81.95	81.95
PGSC0003DMT400005838	Potato	cytosol	81.2	81.2
CDX77662	Canola	cytosol	80.45	80.45
AT4G18100.1	Thale cress	cytosol	80.45	80.45
Bra012616.1-P	Field mustard	cytosol	80.45	80.45
Bra013284.1-P	Field mustard	cytosol	80.45	80.45
Bra021023.1-P	Field mustard	cytosol	80.45	80.45
CDX76545	Canola	cytosol	80.45	80.45
CDX78825	Canola	cytosol	80.45	80.45
CDX90420	Canola	cytosol	80.45	80.45
CDY01520	Canola	extracellular	80.45	80.45
CDY03219	Canola	cytosol	80.45	80.45
CDY61673	Canola	cytosol	80.45	80.45
CDY08886	Canola	cytosol	80.45	80.45
CDY33476	Canola	cytosol	80.45	80.45
CDY35163	Canola	cytosol	80.45	80.45
AT5G46430.1	Thale cress	cytosol	79.7	79.7
Bra017545.1-P	Field mustard	cytosol	79.7	79.7
PGSC0003DMT400054361	Potato	cytosol	45.11	78.95
TraesCS7D01G254200.1	Wheat	cytosol, mitochondrion	87.22	78.38
HORVU5Hr1G075500.3	Barley	cytosol	83.46	75.51
GSMUA_Achr6P12040_001	Banana	cytosol	92.48	75.46
HORVU5Hr1G075420.2	Barley	plastid	84.96	73.86
KRH12807	Soybean	cytosol	58.65	72.9
Zm00001d021018_P002	Maize	cytosol	84.21	72.26
TraesCS7A01G256300.1	Wheat	cytosol	87.22	71.17
TraesCS7D01G257100.1	Wheat	cytosol, mitochondrion	86.47	70.55
Os09t0501700-00	Rice	nucleus	86.47	67.65
TraesCS5D01G286200.2	Wheat	cytosol, mitochondrion	85.71	65.52
TraesCS5B01G278500.2	Wheat	cytosol, mitochondrion	85.71	65.52
Zm00001d031560_P001	Maize	plastid	84.21	54.63
PGSC0003DMT400035383	Potato	cytosol, mitochondrion	80.45	28.31

Protein Annotations

EntrezGene:100527779	MapMan:17.1.2.1.33	EMBL:ACUP02002092	EMBL:BT092652	UniProt:C6T5E0	EnsemblPlantsGene:GLYMA_03G197500
GO:GO:0003674	GO:GO:0003735	GO:GO:0005198	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005829	GO:GO:0005840	GO:GO:0006412	GO:GO:0008150	GO:GO:0008152
GO:GO:0009058	GO:GO:0009987	GO:GO:0019538	GO:GO:0022625	EnsemblPlants:KRH67946	ProteinID:KRH67946
ProteinID:KRH67946.1	PFAM:PD003823	PFAM:PF01655	PANTHER:PTHR23413	InterPro:Ribosomal_L32e	InterPro:Ribosomal_L32e_sf
SMART:SM01393	SUPFAM:SSF52042	UniParc:UPI0001B15D6E	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr3:-:40686782..40689237

Molecular Weight (calculated)

15632.4 Da

IEP (calculated)

11.406

GRAVY (calculated)

-0.826

Length

133 amino acids

Sequence
(BLAST)

001: MAVPLLSKKI VKKRVKKFKR PQSDRKISVK TNWRRPKGID SRVRRKFKGC TLMPNIGYGS DKKTRHYLPN GFKKFVVHNV KDLELLMMHN RTYCAEIAHN
101: ISTRKRKDIV ERAAQLDVVV TNKTARLRTQ EDE

Best Arabidopsis Sequence Match ( AT4G18100.1 )
(BLAST)

001: MAVPLLTKKV VKKRSAKFIR PQSDRRITVK ESWRRPKGID SRVRRKFKGV TLMPNVGYGS DKKTRHYLPN GFKKFVVHNT SELELLMMHN RTYCAEIAHN
101: VSTKKRKAIV ERASQLDVVV TNRLARLRSQ EDE

Arabidopsis Description

RPL32A60S ribosomal protein L32-1 [Source:UniProtKB/Swiss-Prot;Acc:P49211]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.