Factsheet for Os03t0666500-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 1
plastid 2
cytosol 2
plasma membrane 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704
plasma membrane: 28056797

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0141100-02
Os01t0141300-00
Os01t0579600-01
Os01t0711500-00
Os02t0159900-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G19640.1	Os01t0141100-02	AT2G27600.1	17468262
AT4G19640.1	Os01t0141300-00	AT2G27600.1	17468262
AT4G19640.1	Os02t0159900-01	AT2G27600.1	17468262
AT4G19640.1	Os01t0579600-01	AT4G33000.1	17825054
AT4G19640.1	Os01t0711500-00	AT4G33000.1	17825054

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS4A01G294600.1	Wheat	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	94.58	93.66
TraesCS4B01G019100.2	Wheat	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	94.58	93.66
EER93694	Sorghum	cytosol	92.12	92.12
Zm00001d013583_P001	Maize	cytosol	91.63	91.63
TraesCS4D01G017300.1	Wheat	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	94.58	89.3
Os12t0631100-01	Rice	plasma membrane	88.67	88.67
Solyc02g069370.2.1	Tomato	plastid	81.28	82.5
GSMUA_Achr7P16440_001	Banana	cytosol	81.77	82.18
CDY13065	Canola	cytosol	80.79	82.0
CDY23153	Canola	cytosol	80.3	81.5
Bra027593.1-P	Field mustard	cytosol	79.31	80.9
GSMUA_Achr9P10010_001	Banana	cytosol	81.28	80.49
HORVU4Hr1G002980.2	Barley	cytosol	94.58	80.33
CDY39412	Canola	cytosol	78.82	80.0
KRG98019	Soybean	cytosol	78.82	80.0
CDX82579	Canola	cytosol	78.82	80.0
Bra025091.1-P	Field mustard	cytosol	78.82	80.0
AT4G19640.1	Thale cress	cytosol	78.33	79.5
Bra013410.1-P	Field mustard	cytosol	77.34	78.5
CDX99356	Canola	cytosol	77.34	78.11
CDX78949	Canola	cytosol	77.34	78.11
PGSC0003DMT400050774	Potato	cytosol	74.88	76.0
PGSC0003DMT400055259	Potato	cytosol, plastid	74.38	75.5
AT5G45130.2	Thale cress	endoplasmic reticulum	76.35	74.52
Zm00001d034949_P001	Maize	extracellular, mitochondrion	89.66	73.39
GSMUA_Achr6P31600_001	Banana	cytosol	71.92	71.57
KRH20053	Soybean	endoplasmic reticulum	78.82	68.67
CDX78945	Canola	cytosol	15.27	60.78
VIT_04s0079g00760.t01	Wine grape	cytosol	67.49	59.83
Os06t0687100-01	Rice	cytosol	56.65	57.5
CDY09323	Canola	extracellular, plastid, vacuole	30.54	54.39
Os03t0151900-01	Rice	plasma membrane	51.23	52.26
Os10t0441800-01	Rice	plasma membrane	51.23	41.6
Os05t0341600-01	Rice	cytosol, plastid	40.39	38.68
Os07t0496000-01	Rice	plasma membrane	37.44	36.54
Os03t0191400-01	Rice	plasma membrane	38.42	36.28

Protein Annotations

MapMan:22.8.1.6	Gene3D:3.40.50.300	EntrezGene:4333662	ProteinID:AAR87186.1	ProteinID:ABF98072.1	EMBL:AK061203
EMBL:AK067459	ProteinID:BAF12760.1	ProteinID:BAS85640.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0003924	GO:GO:0005488	GO:GO:0005525	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005768	GO:GO:0016787	GO:GO:0030139	EnsemblPlantsGene:Os03g0666500	EnsemblPlants:Os03t0666500-01
InterPro:P-loop_NTPase	PFAM:PF00071	PRINTS:PR00449	PFscan:PS51419	PANTHER:PTHR24073	PANTHER:PTHR24073:SF622
UniProt:Q75HA2	SMART:SM00173	SMART:SM00174	SMART:SM00175	SMART:SM00176	SUPFAM:SSF52540
InterPro:Small_GTP-bd_dom	InterPro:Small_GTPase	TIGRFAMs:TIGR00231	UniParc:UPI000022DFD4	RefSeq:XP_015632579.1	:

Description

small GTP-binding protein OsRab5B1Similar to Ras-related protein RHA1. (Os03t0666500-01);Similar to Ras-related protein RHA1. (Os03t0666500-02);Similar to Ras-related protein RHA1. (Os03t0666500-03)

Coordinates

chr3:+:26234213..26240648

Molecular Weight (calculated)

22138.3 Da

IEP (calculated)

8.181

GRAVY (calculated)

-0.172

Length

203 amino acids

Sequence
(BLAST)

001: MAANAGNKIR NAKLVLLGDV GAGKSSLVLR FVKGQFVEFQ ESTIGAAFFS QTLAVNDETV KFEIWDTAGQ ERYHSLAPMY YRGAAAAIVV YDITNPASFT
101: RAKKWVQELQ AQGNSSTVVA LAGNKADLLE TRQVQIEEAK TYAQENGLFF METSAKTATN VNDIFYEIAK RLLQGQPAQN PQAGMVLSQR PNERLVSSAS
201: CCS

Best Arabidopsis Sequence Match ( AT4G19640.1 )
(BLAST)

001: MAAAGNKSIN AKLVLLGDVG AGKSSLVLRF VKDQFVEFQE STIGAAFFSQ TLAVNDATVK FEIWDTAGQE RYHSLAPMYY RGAAAAIIVF DVTNQASFER
101: AKKWVQELQA QGNPNMVMAL AGNKSDLLDA RKVTAEDAQT YAQENGLFFM ETSAKTATNV KEIFYEIARR LPRVQPTENP TGMVLPDRAM DRAVSSSCCA

Arabidopsis Description

RABF2BRABF2B [Source:UniProtKB/TrEMBL;Acc:A0A178UXV7]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.