Factsheet for Os03t0758800-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, nucleus

Predictor Summary:

nucleus 1
vacuole 1
cytosol 2
plastid 1
mitochondrion 1
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 18260611
nucleus: 23777608
nucleus: 26035336
plasma membrane: 27800704
plastid: 27992503

msms PMID: 23777608 doi

H Mujahid, F Tan, J Zhang, BR Nallamilli, K Pendarvis, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, MS 39762, USA.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 26035336 doi

M Li, X Yin, K Sakata, P Yang, S Komatsu

§Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan., †Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China., ‡National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KXG37504	Sorghum	cytosol	94.41	95.0
GSMUA_Achr6P11110_001	Banana	cytosol	11.18	94.74
Os07t0597000-01	Rice	cytosol, extracellular, plasma membrane	93.17	93.75
KRH74954	Soybean	cytosol	91.93	93.08
KRH70826	Soybean	cytosol	91.93	93.08
Os12t0507200-01	Rice	plasma membrane, plastid	91.93	92.5
VIT_04s0008g06540.t01	Wine grape	cytosol	91.93	92.5
VIT_17s0000g06670.t01	Wine grape	cytosol	91.3	92.45
PGSC0003DMT400028926	Potato	cytosol	90.68	91.25
PGSC0003DMT400007325	Potato	cytosol, mitochondrion	90.06	91.19
KRH03532	Soybean	cytosol	90.06	90.62
KRH54605	Soybean	cytosol	90.06	90.62
KRH56874	Soybean	cytosol	90.06	90.62
KRH63282	Soybean	nucleus	90.06	90.62
KRH72302	Soybean	cytosol	90.06	90.62
VIT_11s0016g00020.t01	Wine grape	cytosol	90.06	90.62
PGSC0003DMT400063132	Potato	cytosol	89.44	90.57
Solyc01g011000.2.1	Tomato	extracellular	89.44	90.57
Solyc04g005510.2.1	Tomato	cytosol, nucleus, plastid	89.44	90.57
Solyc07g005560.2.1	Tomato	cytosol, nucleus, plastid	89.44	90.0
Solyc05g006890.1.1	Tomato	cytosol, nucleus	88.82	89.94
PGSC0003DMT400068977	Potato	cytosol	88.82	89.94
Os07t0112800-01	Rice	nucleus	90.06	89.51
PGSC0003DMT400020252	Potato	cytosol	88.82	89.38
Solyc03g115650.2.1	Tomato	cytosol, extracellular, nucleus	89.44	88.89
Solyc12g010060.1.1	Tomato	nucleus	88.2	88.75
AT1G13950.1	Thale cress	cytosol	86.34	87.97
AT1G69410.1	Thale cress	cytosol	84.47	86.08
CDY71729	Canola	cytosol	83.85	85.44
CDY40919	Canola	cytosol	83.85	85.44
CDX95937	Canola	cytosol	83.85	85.44
Bra004379.1-P	Field mustard	cytosol	83.23	84.81
KRH00098	Soybean	cytosol	40.99	83.54
Bra024702.1-P	Field mustard	cytosol	80.12	81.13
AT1G26630.1	Thale cress	cytosol	78.26	79.25
KRH00097	Soybean	cytosol	34.78	75.68
CDY13072	Canola	cytosol	39.13	70.0
CDY03674	Canola	cytosol	43.48	63.64
Bra007992.1-P	Field mustard	plastid	23.6	60.32
CDY36262	Canola	cytosol	32.3	31.33
KRH03058	Soybean	vacuole	70.81	29.01
CDX83676	Canola	cytosol	31.06	28.74
KRH64023	Soybean	plastid	17.39	19.58

Protein Annotations

MapMan:17.5.3.1	Gene3D:2.30.30.30	Gene3D:2.40.50.140	ProteinID:AAK16176.1	ProteinID:AAK63944.1	ProteinID:ABF98985.1
EMBL:AK060387	EMBL:AK121592	ProteinID:BAF13250.1	ProteinID:BAS86487.1	GO:GO:0003674	GO:GO:0003676
GO:GO:0003723	GO:GO:0003743	GO:GO:0003746	GO:GO:0005488	GO:GO:0006412	GO:GO:0006413
GO:GO:0006452	GO:GO:0008135	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987
GO:GO:0016043	GO:GO:0019538	GO:GO:0043022	GO:GO:0045901	GO:GO:0045905	InterPro:IF5A-like
InterPro:IPR014722	InterPro:NA-bd_OB-fold	EnsemblPlantsGene:Os03g0758800	EnsemblPlants:Os03t0758800-01	PFAM:PF01287	PIRSF:PIRSF003025
ScanProsite:PS00302	PANTHER:PTHR11673	PANTHER:PTHR11673:SF22	UniProt:Q9AUW3	InterPro:Rib_L2_dom2	SMART:SM01376
SUPFAM:SSF50104	SUPFAM:SSF50249	TIGRFAMs:TIGR00037	InterPro:Trans_elong_IF5A_hypusine_site	InterPro:Transl_elong_IF5A_C	InterPro:Translation_prot_SH3-like_sf
UniParc:UPI000009F703	:	:	:	:	:

Description

Similar to Eukaryotic translation initiation factor 5A-2 (eIF-5A-2) (eIF-4D). (Os03t0758800-01)

Coordinates

chr3:+:31388386..31390881

Molecular Weight (calculated)

17545.7 Da

IEP (calculated)

6.077

GRAVY (calculated)

-0.474

Length

161 amino acids

Sequence
(BLAST)

001: MSDSEEHHFE SKADAGASKT YPQQAGTIRK NGHIVIKNRP CKVVEVSTSK TGKHGHAKCH FVAIDIFNGK KLEDIVPSSH NCDVPHVNRT DYQLIDISED
101: GFVSLLTESG GTKDDLRLPS DEALLTQIKD GFAEGKDLIV TVMSAMGEEQ ICALKDIGPK N

Best Arabidopsis Sequence Match ( AT1G13950.1 )
(BLAST)

001: MSDEEHHFES SDAGASKTYP QQAGTIRKNG YIVIKNRPCK VVEVSTSKTG KHGHAKCHFV AIDIFTSKKL EDIVPSSHNC DVPHVNRTDY QLIDISEDGY
101: VSLLTDNGST KDDLKLPNDD TLLQQIKSGF DDGKDLVVSV MSAMGEEQIN ALKDIGPK

Arabidopsis Description

ELF5A-1Eukaryotic translation initiation factor 5A [Source:UniProtKB/TrEMBL;Acc:A0A178WBN7]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.