Factsheet for Os12t0507200-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, plastid

Predictor Summary:

nucleus 1
vacuole 1
cytosol 3
mitochondrion 2
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704
plastid: 27992503

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Os07t0597000-01	Rice	cytosol, extracellular, plasma membrane	96.25	96.25
VIT_17s0000g06670.t01	Wine grape	cytosol	93.75	94.34
VIT_11s0016g00020.t01	Wine grape	cytosol	93.75	93.75
KRH70826	Soybean	cytosol	93.12	93.71
TraesCS5A01G104800.1	Wheat	cytosol	93.12	93.12
TraesCS5B01G108600.1	Wheat	plastid	93.12	93.12
Solyc01g011000.2.1	Tomato	extracellular	92.5	93.08
Solyc04g005510.2.1	Tomato	cytosol, nucleus, plastid	92.5	93.08
PGSC0003DMT400007325	Potato	cytosol, mitochondrion	92.5	93.08
PGSC0003DMT400068977	Potato	cytosol	92.5	93.08
KRH74954	Soybean	cytosol	91.88	92.45
Os03t0758800-01	Rice	nucleus, plasma membrane	92.5	91.93
Solyc12g010060.1.1	Tomato	nucleus	91.88	91.88
KRH03532	Soybean	cytosol	91.88	91.88
KRH54605	Soybean	cytosol	91.88	91.88
KRH56874	Soybean	cytosol	91.88	91.88
KRH63282	Soybean	nucleus	91.88	91.88
PGSC0003DMT400063132	Potato	cytosol	91.25	91.82
Solyc05g006890.1.1	Tomato	cytosol, nucleus	90.62	91.19
PGSC0003DMT400028926	Potato	cytosol	90.62	90.62
PGSC0003DMT400020252	Potato	cytosol	90.62	90.62
VIT_04s0008g06540.t01	Wine grape	cytosol	90.62	90.62
KRH72302	Soybean	cytosol	90.62	90.62
AT1G13950.1	Thale cress	cytosol	89.38	90.51
Solyc03g115650.2.1	Tomato	cytosol, extracellular, nucleus	91.25	90.12
Solyc07g005560.2.1	Tomato	cytosol, nucleus, plastid	90.0	90.0
Os07t0112800-01	Rice	nucleus	90.0	88.89
KRH00098	Soybean	cytosol	42.5	86.08
Bra004379.1-P	Field mustard	cytosol	85.0	86.08
CDY71729	Canola	cytosol	85.0	86.08
CDY40919	Canola	cytosol	85.0	86.08
CDX95937	Canola	cytosol	85.0	86.08
TraesCS5D01G116200.2	Wheat	cytosol	92.5	85.55
AT1G69410.1	Thale cress	cytosol	84.38	85.44
Bra024702.1-P	Field mustard	cytosol	81.25	81.76
AT1G26630.1	Thale cress	cytosol	79.38	79.87
KRH00097	Soybean	cytosol	35.62	77.03
HORVU5Hr1G028360.3	Barley	mitochondrion	93.12	74.5
CDY13072	Canola	cytosol	38.75	68.89
CDY03674	Canola	cytosol	43.12	62.73
Bra007992.1-P	Field mustard	plastid	23.12	58.73
KRH03058	Soybean	vacuole	70.0	28.5
CDY36262	Canola	cytosol	29.38	28.31
CDX83676	Canola	cytosol	30.0	27.59
KRH64023	Soybean	plastid	16.88	18.88

Protein Annotations

MapMan:17.5.3.1	Gene3D:2.30.30.30	Gene3D:2.40.50.140	EntrezGene:4352300	ProteinID:ABA98689.1	EMBL:AK061867
EMBL:AK099039	ProteinID:BAF29862.1	ProteinID:BAT17298.1	ProteinID:EEE53277.1	GO:GO:0003674	GO:GO:0003676
GO:GO:0003723	GO:GO:0003743	GO:GO:0003746	GO:GO:0005488	GO:GO:0006412	GO:GO:0006413
GO:GO:0006452	GO:GO:0008135	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987
GO:GO:0016043	GO:GO:0019538	GO:GO:0043022	GO:GO:0045901	GO:GO:0045905	InterPro:IF5A-like
InterPro:IPR014722	InterPro:NA-bd_OB-fold	EnsemblPlantsGene:Os12g0507200	EnsemblPlants:Os12t0507200-01	PFAM:PF01287	PIRSF:PIRSF003025
ScanProsite:PS00302	PANTHER:PTHR11673	PANTHER:PTHR11673:SF22	UniProt:Q2QQ48	InterPro:Rib_L2_dom2	SMART:SM01376
SUPFAM:SSF50104	SUPFAM:SSF50249	TIGRFAMs:TIGR00037	InterPro:Trans_elong_IF5A_hypusine_site	InterPro:Transl_elong_IF5A_C	InterPro:Translation_prot_SH3-like_sf
UniParc:UPI0000681015	RefSeq:XP_015620359.1	:	:	:	:

Description

EUKARYOTIC INITIATION FACTOR 4DSimilar to Eukaryotic translation initiation factor 5A-2 (eIF-5A-2) (eIF-4D). (Os12t0507200-01)

Coordinates

chr12:+:19462695..19465623

Molecular Weight (calculated)

17557.7 Da

IEP (calculated)

5.869

GRAVY (calculated)

-0.497

Length

160 amino acids

Sequence
(BLAST)

001: MSDSEEHHFE SKADAGASKT YPQQAGTIRK NGYIVIKNRP CKVVEVSTSK TGKHGHAKCH FVAIDIFTAK KLEDIVPSSH NCDVPHVNRT EYQLIDISED
101: GFVSLLTENG NTKDDLRLPT DDNLLSQIKD GFGEGKDLVV TVMSAMGEEQ ICALKDIGPK

Best Arabidopsis Sequence Match ( AT1G13950.1 )
(BLAST)

001: MSDEEHHFES SDAGASKTYP QQAGTIRKNG YIVIKNRPCK VVEVSTSKTG KHGHAKCHFV AIDIFTSKKL EDIVPSSHNC DVPHVNRTDY QLIDISEDGY
101: VSLLTDNGST KDDLKLPNDD TLLQQIKSGF DDGKDLVVSV MSAMGEEQIN ALKDIGPK

Arabidopsis Description

ELF5A-1Eukaryotic translation initiation factor 5A [Source:UniProtKB/TrEMBL;Acc:A0A178WBN7]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.