CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 1
plastid 3
cytosol 3

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, nucleus, plastid
BaCelLo:	nucleus
ChloroP:	plastid
MultiLoc:	cytosol
PProwler:	plastid
TargetP:	plastid
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
CDX73678
CDX78797
CDX92401
CDX95314
CDY23174
CDY60785

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G19100.1	CDX73678	AT3G51920.1	17360592
AT3G19100.1	CDX95314	AT3G51920.1	17360592
AT3G19100.1	CDY23174	AT3G51920.1	17360592
AT3G19100.1	CDX78797	AT4G17750.1	18466301
AT3G19100.1	CDY60785	AT4G17750.1	18466301
AT3G19100.1	CDX92401	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY22990	Canola	cytosol	95.44	95.12
CDY18568	Canola	cytosol, plastid	89.86	89.56
CDX92303	Canola	cytosol	90.03	89.43
CDX95464	Canola	cytosol, plastid	89.02	89.02
CDX99267	Canola	cytosol	89.53	88.93
Bra001734.1-P	Field mustard	cytosol, plastid	97.3	88.21
AT3G19100.1	Thale cress	cytosol	88.34	87.31
CDX77233	Canola	cytosol, plastid	77.7	77.83
CDY16510	Canola	cytosol, plastid	77.87	77.35
Os07t0619800-02	Rice	cytosol, plasma membrane	56.93	74.39
TraesCS2D01G173100.1	Wheat	cytosol	57.77	71.85
VIT_03s0063g00940.t01	Wine grape	cytosol	70.1	71.06
KRH71170	Soybean	cytosol	71.11	70.4
KRH50248	Soybean	cytosol	70.78	70.07
CDY69753	Canola	cytosol, plastid	62.84	69.66
Solyc01g108400.2.1	Tomato	cytosol	68.58	69.05
PGSC0003DMT400066365	Potato	cytosol	67.91	68.37
CDY65651	Canola	cytosol	67.4	68.21
GSMUA_Achr1P21380_001	Banana	cytosol	69.76	68.04
CDY65016	Canola	cytosol	67.74	67.51
CDY52824	Canola	cytosol	67.74	67.51
GSMUA_Achr2P07660_001	Banana	cytosol	68.24	66.56
CDY22505	Canola	cytosol	62.16	64.79
EER99712	Sorghum	cytosol	65.71	64.73
Zm00001d006885_P002	Maize	cytosol	62.67	64.63
CDY35427	Canola	cytosol	61.49	64.2
CDX79956	Canola	cytosol	62.33	63.95
TraesCS2A01G165800.1	Wheat	cytosol, plastid	64.86	63.68
TraesCS2B01G191800.1	Wheat	cytosol	64.86	63.58
CDY23873	Canola	cytosol	61.99	63.49
CDY47375	Canola	cytosol, nucleus, plasma membrane	61.32	63.24
Zm00001d000221_P001	Maize	cytosol	12.16	63.16
CDX76010	Canola	cytosol	60.3	62.63
Zm00001d022166_P001	Maize	cytosol	57.09	58.99
CDY57585	Canola	cytosol, mitochondrion	40.37	57.73
CDY27530	Canola	cytosol	56.93	56.54
CDY48019	Canola	cytosol	56.93	56.54
CDY06943	Canola	cytosol	43.58	55.36
CDX87128	Canola	cytosol	53.72	54.83
CDY19836	Canola	cytosol	53.72	54.83
CDY08129	Canola	cytosol	54.9	54.62
CDX88104	Canola	cytosol	53.21	54.03
CDY05930	Canola	cytosol	54.56	54.01
CDX80445	Canola	cytosol	52.03	52.83
CDX86978	Canola	cytosol	54.73	49.92
CDY00200	Canola	cytosol	54.56	49.46
HORVU2Hr1G030520.1	Barley	cytosol	40.03	47.88
CDX87112	Canola	cytosol	22.64	46.21
CDY30782	Canola	plastid	39.02	44.17
CDY45213	Canola	plastid	39.53	44.07
CDX81407	Canola	plastid	39.53	43.25
CDY18593	Canola	plastid	39.02	42.86
CDY38141	Canola	cytosol, plastid	41.05	42.33
CDY47052	Canola	cytosol	38.51	42.14
CDX72575	Canola	cytosol	38.18	41.77
CDY63401	Canola	cytosol, plastid	40.54	41.67
CDY07808	Canola	plastid	40.88	41.44
CDY29208	Canola	plastid	40.71	41.41
CDY48773	Canola	cytosol	30.07	37.55
CDY40640	Canola	cytosol	14.02	37.05
CDX92529	Canola	cytosol	32.77	36.33
CDY14001	Canola	cytosol	31.93	35.66
CDY42266	Canola	cytosol	32.09	35.58
CDY44918	Canola	cytosol	31.93	35.39
CDY53966	Canola	cytosol	31.76	35.34
CDX97205	Canola	cytosol	31.93	35.33
CDY14793	Canola	cytosol	28.89	35.04
CDX79906	Canola	cytosol	31.42	34.96
CDX67695	Canola	cytosol	31.42	34.7
CDY21691	Canola	cytosol, plastid	31.93	34.55
CDY06900	Canola	cytosol	30.91	34.46
CDX81903	Canola	cytosol, plastid	31.59	34.44
CDY33817	Canola	cytosol	31.25	34.39
CDX73684	Canola	cytosol	30.57	34.28
CDX78044	Canola	cytosol, nucleus, plasma membrane	30.41	34.09
CDX95303	Canola	cytosol	30.41	34.09
CDY23165	Canola	cytosol	30.41	34.09
CDY29049	Canola	cytosol	30.41	34.09
CDY45391	Canola	cytosol	31.42	34.07
CDX73108	Canola	cytosol	31.42	34.0
CDY16652	Canola	cytosol	27.03	33.97
CDY10809	Canola	cytosol	30.74	33.89
CDX90631	Canola	cytosol	30.24	33.84
CDX72022	Canola	cytosol	30.74	33.7
CDY34489	Canola	cytosol	30.57	33.64
CDY06098	Canola	cytosol	30.74	33.64
CDX84782	Canola	cytosol, plastid	30.91	33.27
CDY23939	Canola	cytosol, plastid	30.91	33.27
CDY19084	Canola	plastid	31.25	32.69
CDY15391	Canola	plastid	31.59	32.24
CDY26714	Canola	cytosol	31.59	30.71
CDY19259	Canola	cytosol, plastid	31.59	30.51
CDY71104	Canola	cytosol	6.76	28.57
CDY10803	Canola	cytosol	20.44	27.19
CDY24762	Canola	cytosol	8.78	23.01
CDX94570	Canola	cytosol	26.52	21.16

Protein Annotations

Gene3D:1.10.238.10	Gene3D:1.10.510.10	MapMan:18.4.5.5	Gene3D:3.30.200.20	GO:A0A078CYP3	EnsemblPlants:CDX82311
ProteinID:CDX82311	ProteinID:CDX82311.1	InterPro:EF-hand-dom_pair	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0004672	GO:GO:0005488	GO:GO:0005524	GO:GO:0006464	GO:GO:0006468	GO:GO:0008150
GO:GO:0008152	GO:GO:0009987	GO:GO:0016301	GO:GO:0016740	GO:GO:0019538	EnsemblPlantsGene:GSBRNA2T00137423001
InterPro:IPR000719	InterPro:Kinase-like_dom_sf	PFAM:PF00069	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011
PANTHER:PTHR24349	PANTHER:PTHR24349:SF115	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF47473
SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI0004EF0DFF	:	:	:

Description

BnaA03g35100D

Coordinates

chrLK031828:-:512951..515437

Molecular Weight (calculated)

66240.5 Da

IEP (calculated)

8.227

GRAVY (calculated)

-0.255

Length

592 amino acids

Sequence
(BLAST)

001: MGGCTSKPST SVKHNPHTPS EAIPQNVDST PARLENSPAH TSTTVKASPF FPFYTPSPAK HRRNKSVGGE SKSTTSTPLR QLARAFHPPS PAKHIRDVLR
101: RRKGKEATLP AESKSEEEEV GLDKRFGYSK EFESRMELGE EIGRGHFGYT CSAKFKKGEL KDLEVAVKVI PKSKMTTAIS IEDVRREVKI LRALSGHNNL
201: VQFYDVFEDN DNVYIAMELC DGGELLDRIL ARGGKYSEDD AKEVLIQILN VVAFCHLQGV VHRDLKPENF LYTSKEENSQ LKVIDFGLSD FVRPHERLND
301: IVGSAYYVAP EVLHRSYTTE ADVWSIGVIA YILLCGSRPF WARTESGIFR AVLKADPSFD VPPWPSLSLE AKDFVKRLLY KDPRKRMTAS QALMHPWIAG
401: CIKNMSIPFD ILIFKQIKAY LKSSSLRKAA LMALSKTLIT DEVLYLKAQF ALLAPNINGL ITLDNIASAL AINATEVMKE SRIPDFVALL NGLQNKGMDF
501: EEFCAASISV HQHESLDCWE QSVHHAYELF DMNGNRVIVI EELASELGFG SSVPVHTILH DWIRHTDGKL SFLGFVKVLH GVSTRQPLAK TR

Best Arabidopsis Sequence Match ( AT1G49580.1 )
(BLAST)

001: MGGCTSKPST SSGRPNPFAP GNDYPQIDDF APDHPGKSPI PTPSAAKASP FFPFYTPSPA RHRRNKSRDV GGGGESKSLT STPLRQLRRA FHPPSPAKHI
101: RAALRRRKGK KEAALSGVTQ LTTEVPQREE EEEVGLDKRF GFSKEFHSRV ELGEEIGRGH FGYTCSAKFK KGELKGQVVA VKIIPKSKMT TAIAIEDVRR
201: EVKILQALSG HKNLVQFYDA FEDNANVYIA MELCEGGELL DRILARGGKY SENDAKPVII QILNVVAFCH FQGVVHRDLK PENFLYTSKE ENSQLKAIDF
301: GLSDFVRPDE RLNDIVGSAY YVAPEVLHRS YTTEADVWSI GVIAYILLCG SRPFWARTES GIFRAVLKAD PSFDEPPWPF LSSDAKDFVK RLLFKDPRRR
401: MSASQALMHP WIRAYNTDMN IPFDILIFRQ MKAYLRSSSL RKAALRALSK TLIKDEILYL KTQFSLLAPN KDGLITMDTI RMALASNATE AMKESRIPEF
501: LALLNGLQYR GMDFEEFCAA AINVHQHESL DCWEQSIRHA YELFDKNGNR AIVIEELASE LGVGPSIPVH SVLHDWIRHT DGKLSFFGFV KLLHGVSVRA
601: SGKTTR

Arabidopsis Description

CRK8CDPK-related kinase 8 [Source:UniProtKB/Swiss-Prot;Acc:Q9FX86]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.