CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, cytosol

Predictor Summary:

nucleus 2
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

endoplasmic reticulum: 27224218

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG90331
KRG94459
KRG97711	plasma membrane
KRH02873
KRH05944	golgi
KRH06999
KRH07810
KRH14933	plasma membrane
KRH17427	golgi
KRH17907
KRH24097
KRH27813
KRH29071
KRH31163	plasma membrane
KRH39402
KRH73205	plasma membrane
KRH77672

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G22770.1	KRH05944	AT1G47830.1	23975899
AT5G22770.1	KRH17427	AT1G47830.1	23975899
AT5G22770.1	KRH05944	AT1G47830.1	23975899
AT5G22770.1	KRH17427	AT1G47830.1	23975899
AT5G22770.1	KRG94459	AT3G01780.1	24529374
AT5G22770.1	KRH06999	AT3G01780.1	24529374
AT5G22770.1	KRG97711	AT3G11130.1	23975898
AT5G22770.1	KRH14933	AT3G11130.1	23975898
AT5G22770.1	KRH31163	AT3G11130.1	23975898
AT5G22770.1	KRH73205	AT3G11130.1	23975898
AT5G22770.1	KRH24097	AT4G23460.1	23975899
AT5G22770.1	KRH27813	AT4G23460.1	23975899
AT5G22770.1	KRH29071	AT4G23460.1	23975899
AT5G22770.1	KRH77672	AT4G23460.1	23975899
AT5G22770.1	KRG90331	AT5G57460.1	24529374
AT5G22770.1	KRH02873	AT5G57460.1	24529374
AT5G22770.1	KRH07810	AT5G65960.1	23975899
AT5G22770.1	KRH39402	AT5G65960.1	23975899
AT5G22780.1	KRH17907	AT1G05020.1	15054111

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH26563	Soybean	endoplasmic reticulum	97.65	97.65
Solyc11g066760.1.1	Tomato	cytosol	42.84	93.58
CDY20343	Canola	cytosol	28.04	91.67
VIT_06s0061g01170.t01	Wine grape	cytosol	88.73	89.16
Solyc06g074650.2.1	Tomato	plastid	85.69	85.94
CDY26951	Canola	cytosol	81.86	82.84
Bra002427.1-P	Field mustard	cytosol	81.76	82.82
CDX92339	Canola	cytosol	81.76	82.82
Bra037603.1-P	Field mustard	cytosol	82.25	82.74
AT5G22770.1	Thale cress	cytosol	81.96	82.61
AT5G22780.1	Thale cress	cytosol	81.67	82.23
CDY29719	Canola	cytosol	75.49	78.49
Solyc11g066770.1.1	Tomato	cytosol	32.94	77.42
CDY20344	Canola	cytosol	48.14	76.12
KRH25154	Soybean	cytosol	28.33	71.01
KRH74623	Soybean	mitochondrion	21.76	25.49
KRH69527	Soybean	mitochondrion	21.76	25.43
KRH45711	Soybean	cytosol, mitochondrion	21.08	24.4
KRH76456	Soybean	nucleus	17.25	18.22
KRH07246	Soybean	nucleus	16.27	16.92
KRH07767	Soybean	nucleus	16.18	16.82
KRH39774	Soybean	nucleus	16.86	16.73
KRG99303	Soybean	nucleus	2.94	16.67
KRH41691	Soybean	cytosol	14.02	15.61
KRH60392	Soybean	cytosol	14.8	15.46

Protein Annotations

Gene3D:1.25.10.10	EntrezGene:100798273	Gene3D:2.60.40.1230	MapMan:22.1.3.1	Gene3D:3.30.310.10	EMBL:ACUP02008432
InterPro:AP2_complex_asu	InterPro:ARM-like	InterPro:ARM-type_fold	InterPro:Clathrin/coatomer_adapt-like_N	InterPro:Clathrin_a-adaptin_app_sub_C	InterPro:Clathrin_a/b/g-adaptin_app_Ig
InterPro:Clathrin_app_Ig-like_sf	InterPro:Coatomer/calthrin_app_sub_C	EnsemblPlantsGene:GLYMA_13G320200	GO:GO:0003674	GO:GO:0005215	GO:GO:0005488
GO:GO:0005515	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005905
GO:GO:0006810	GO:GO:0006886	GO:GO:0006897	GO:GO:0008150	GO:GO:0008565	GO:GO:0015031
GO:GO:0016020	GO:GO:0016192	GO:GO:0030117	GO:GO:0030131	GO:GO:0035615	GO:GO:0072583
UniProt:I1MS73	InterPro:IPR011989	InterPro:IPR012295	EnsemblPlants:KRH22743	ProteinID:KRH22743	ProteinID:KRH22743.1
PFAM:PF01602	PFAM:PF02296	PFAM:PF02883	PIRSF:PIRSF037091	PANTHER:PTHR22780	PANTHER:PTHR22780:SF4
SMART:SM00809	SUPFAM:SSF48371	SUPFAM:SSF49348	SUPFAM:SSF55711	InterPro:TBP_dom_sf	UniParc:UPI000233E3DE
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr13:-:41460580..41472184

Molecular Weight (calculated)

113612.0 Da

IEP (calculated)

6.440

GRAVY (calculated)

-0.097

Length

1020 amino acids

Sequence
(BLAST)

0001: MAMSGMRGLS VFISDIRNCQ NKEQERLRVD KELGNIRTRF KNEKALTPYE KKKYVWKMLY IYMLGYDVDF GHMEAVSLIS APKYPEKQVG YIVTSSLLNE
0101: NHDFLRLAIN TVRNDIIGRN ETFQCLALTM VGNIGGREFA ESLAPDVQKL LISSSCRPLV RKKAALCLLR LYRKNPDVVN VDGWADRMAQ LLDERDLGVL
0201: TSSMSLLVAL VSNNHEAYWS CLPKCIKILE RLARNQDIPQ EYTYYGIPSP WLQVKTMRAL QYFPTIEDPN ARRSLFEVLQ RILMGTDVVK NVNKNNASHA
0301: VLFEALALVM HLDAEKEMMS QCVALLGKFI AVREPNIRYL GLENMTRMLM VTDVQDIIKR HQAQIITSLK DPDISIRRRA LDLLYGMCDV SNAKDIVEEL
0401: LQYLSTAEFA MREELSLKAA ILAEKFAPDL SWYVDVILQL IDKAGDFVSD DIWFRVVQFV TNNEDLQPYA AAKAREYLDK PAIHETMVKV SAYILGEFGH
0501: LLARRPGCSP KELFSIIHEK LPTVSTSTIS ILLSTYAKIL MHSQPPDPEL QNQIWTIFKK YESSIEVEIQ QRSVEYFALS RKGAALMDIL AEMPKFPERQ
0601: SALIKKAEDT EVDTAELSAI KLRAQQQSQT SNALVVTGQS HANGTPPVGQ LSLVKVPSMS SNADEADQRL SQENGTLSKV DSQPPSADLL GDLLGPLAIE
0701: GPPGISVHPQ PSSNSGLEGT VVEATAIVPA GEQANSVQPI GNIAERFHAL CMKDSGVLYE DPYIQIGIKA EWRAHQGHLV LFLGNKNTSP LVSVQALILH
0801: PTHLKMELSL VPETIPPRAQ VQCPLEVINL HPSRDVAVLD FSYKFGNNMV NVKLRLPAVL NKFLQPITIS AEEFFPQWRS LPGPPLKLQE VVRGVRPLPL
0901: LEMANLFNSF HLTVCPGLDP NPNNLVASTT FYSESTRAML CLARIETDPA DRTQLRMTVA SGDPTLTFEL KEFIKDQLVS IPTAATHVPT QPAPTSPPVA
1001: QPGSAPTALT DPGAMLAALL

Best Arabidopsis Sequence Match ( AT5G22780.1 )
(BLAST)

0001: MTGMRGLSVF ISDVRNCQNK EAERLRVDKE LGNIRTCFKN EKVLTPYKKK KYVWKMLYIH MLGYDVDFGH MEAVSLISAP KYPEKQVGYI VTSCLLNENH
0101: DFLKLAINTV RNDIIGRNET FQCLALTLVG NIGGRDFAES LAPDVQKLLI SSSCRPLVRK KAALCLLRLF RKNPDAVNVD GWADRMAQLL DERDLGVLTS
0201: STSLLVALVS NNHEAYSSCL PKCVKILERL ARNQDVPQEY TYYGIPSPWL QVKAMRALQY FPTIEDPSTR KALFEVLQRI LMGTDVVKNV NKNNASHAVL
0301: FEALSLVMHL DAEKEMMSQC VALLGKFISV REPNIRYLGL ENMTRMLMVT DVQDIIKKHQ SQIITSLKDP DISIRRRALD LLYGMCDVSN AKDIVEELLQ
0401: YLSTAEFSMR EELSLKAAIL AEKFAPDLSW YVDVILQLID KAGDFVSDDI WFRVVQFVTN NEDLQPYAAS KAREYMDKIA IHETMVKVSA YILGEYGHLL
0501: ARQPGCSASE LFSILHEKLP TVSTPTIPIL LSTYAKLLMH AQPPDPELQK KVWAVFKKYE SCIDVEIQQR AVEYFELSKK GPAFMDVLAE MPKFPERQSS
0601: LIKKAENVED TADQSAIKLR AQQQPSNAIV LADPQPVNGA PPPLKVPILS GSTDPESVAR SLSHPNGTLS NIDPQTPSPD LLSDLLGPLA IEAPPGAVSY
0701: EQHGPVGAEG VPDEIDGSAI VPVEEQTNTV ELIGNIAERF HALCLKDSGV LYEDPHIQIG IKAEWRGHHG RLVLFMGNKN TSPLTSVQAL ILPPAHLRLD
0801: LSPVPDTIPP RAQVQSPLEV MNIRPSRDVA VLDFSYKFGT NVVSAKLRIP ATLNKFLQPL QLTSEEFFPQ WRAISGPPLK LQEVVRGVRP LALPEMANLF
0901: NSFHVTICPG LDPNPNNLVA STTFYSETTG AMLCLARIET DPADRTQLRL TVGSGDPTLT FELKEFIKEQ LITIPMGSRA LVPAAGPAPS PAVQPPSPAA
1001: LADDPGAMLA GLL

Arabidopsis Description

ALPHAC-ADAP-2 complex subunit alpha-2 [Source:UniProtKB/Swiss-Prot;Acc:Q8LPK4]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.