CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

cytosol 4
plastid 2
nucleus 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH29421	Soybean	cytosol	94.72	95.08
CDX72487	Canola	cytosol	30.94	87.7
KRH60811	Soybean	nucleus	82.26	82.26
PGSC0003DMT400001937	Potato	cytosol	77.55	77.99
Bra011543.1-P	Field mustard	cytosol	72.64	77.15
Solyc01g109830.2.1	Tomato	cytosol	76.23	76.66
KRH51504	Soybean	nucleus	81.7	76.23
CDX89705	Canola	cytosol	73.77	76.07
CDY45317	Canola	cytosol	70.75	75.45
Bra017662.1-P	Field mustard	cytosol	71.7	75.4
AT2G16370.1	Thale cress	cytosol	73.4	74.95
Os12t0446900-01	Rice	cytosol	73.21	74.47
Zm00001d007318_P001	Maize	cytosol	73.4	74.38
Os11t0484400-01	Rice	cytosol, plasma membrane, plastid	72.64	73.9
CDX69070	Canola	mitochondrion, plastid	73.21	70.55
VIT_18s0075g01010.t01	Wine grape	cytosol, mitochondrion	11.51	70.11
AT4G34570.1	Thale cress	mitochondrion	73.77	69.2
CDX75443	Canola	mitochondrion	73.21	68.19
KXG28376	Sorghum	mitochondrion, plastid	74.15	67.53
Zm00001d049188_P008	Maize	mitochondrion	73.96	67.35
Zm00001d030396_P001	Maize	cytosol	35.66	66.32
Zm00001d050448_P001	Maize	cytosol	35.47	65.96
TraesCS2A01G518600.1	Wheat	mitochondrion, plastid	71.32	65.62
TraesCS2D01G520000.1	Wheat	plastid	71.13	65.45
TraesCS2B01G547000.1	Wheat	plastid	71.13	65.34
GSMUA_Achr2P14300_001	Banana	cytosol	60.38	63.37
Bra013079.1-P	Field mustard	cytosol	72.26	61.97
HORVU2Hr1G114640.8	Barley	cytosol, plastid	70.75	61.88
CDY02264	Canola	cytosol	72.08	61.81
CDX83194	Canola	cytosol	47.36	58.64
Bra000581.1-P	Field mustard	cytosol	49.62	58.19
AT2G21550.1	Thale cress	cytosol, plasma membrane, plastid	52.83	56.91
CDY21464	Canola	cytosol	30.57	53.29
Os11t0615250-00	Rice	cytosol	8.3	39.64

Protein Annotations

KEGG:00240+2.1.1.45	KEGG:00670+1.5.1.3	KEGG:00670+1.5.1.3+2.1.1.45	KEGG:00670+2.1.1.45	KEGG:00790+1.5.1.3	EntrezGene:100788658
Gene3D:3.30.572.10	Gene3D:3.40.430.10	MapMan:6.3.1.2	MapMan:7.5.5	EMBL:ACUP02007176	InterPro:DHFR-TS
InterPro:DHFR-like_dom_sf	InterPro:DHFR_dom	EnsemblPlantsGene:GLYMA_12G041000	GO:GO:0003674	GO:GO:0003824	GO:GO:0004146
GO:GO:0004799	GO:GO:0006139	GO:GO:0006231	GO:GO:0006545	GO:GO:0006730	GO:GO:0008150
GO:GO:0008152	GO:GO:0008168	GO:GO:0009058	GO:GO:0009165	GO:GO:0009987	GO:GO:0016491
GO:GO:0016740	GO:GO:0032259	GO:GO:0046654	GO:GO:0055114	UniProt:I1LQ11	InterPro:IPR001796
InterPro:IPR024072	InterPro:IPR036926	EnsemblPlants:KRH24440	ProteinID:KRH24440	ProteinID:KRH24440.1	ProteinID:KRH24441.1
HAMAP:MF_00008	PFAM:PF00186	PFAM:PF00303	PIRSF:PIRSF000389	PRINTS:PR00108	ScanProsite:PS00091
PFscan:PS51330	PANTHER:PTHR11548	PANTHER:PTHR11548:SF7	MetaCyc:PWY-3841	MetaCyc:PWY-6614	MetaCyc:PWY-7184
MetaCyc:PWY-7187	MetaCyc:PWY-7198	MetaCyc:PWY-7199	MetaCyc:PWY-7210	SUPFAM:SSF53597	SUPFAM:SSF55831
TIGRFAMs:TIGR03284	InterPro:Thymidate_synth/dCMP_Mease	InterPro:Thymidate_synth/dCMP_Mease_sf	InterPro:Thymidylate_synthase	InterPro:Thymidylate_synthase_AS	UniParc:UPI000233D053
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr12:+:2988069..2993157

Molecular Weight (calculated)

59905.7 Da

IEP (calculated)

6.833

GRAVY (calculated)

-0.280

Length

530 amino acids

Sequence
(BLAST)

001: MATDALVISN GNGNGNAKSE LHPQRTYQVV VVATRDMGIS KDAKLPWTLP TELKFFEEIT TTTSDPGKKN AVVMGRKTWE SIPPENRPLP GRLNVVLTRS
101: GSFDIATAEN VLICGSMASA MELLASSPYC LSIEKVFLTG GGEIFREALN APGCEAAHIT EIEASIECDT FMPRVDTNVF QTWYSSFPLV EDNLRYSFTT
201: YARVRSSPPE FLDQNADPFF YNNSDSFKFE VKKFSFLPKM VYERHEEFKY LRLVQEIISE GTTKDDRTGT GTLSKFGCQM RFNLRRNFPL LTTKKVFWRG
301: VVEELLWFIS GSTSAKALQE KGIHIWDGNA SREYLDSIGL TEREKGDLGP VYGFQWRHFG AKYTDMHADY SGQGFDQLLD VINKIKHNPN DRRIILSAWN
401: PSDLKLMALP PCHMFAQFYV ANGELSCQMY QRSADMGLGV PFNIASYALL TCMIAHVCDL VPGDFIHVIG DAHVYRNHVR PLQEQLQNQP KPFPILKINP
501: KKKDIDSFVA ADFKLIGYDP HQKIEMKMAV

Best Arabidopsis Sequence Match ( AT2G16370.1 )
(BLAST)

001: MATTTLNDSV TTTLASEPQR TYQVVVAATK EMGIGKDGKL PWNLPTDLKF FKDITLTTSD SSKKNAVVMG RKTWESIPIK YRPLSGRLNV VLTRSGGFDI
101: ANTENVVTCS SVDSALDLLA APPYCLSIER VFVIGGGDIL REALNRPSCD AIHLTEIDTS VDCDTFIPAI DTSVYQPWSS SFPVTENGLR FCFTTFVRVK
201: SSADESSDES NGSQSLQFDG KKFLFLPKMV FDQHEEFLYL NMVEDIISNG NVKNDRTGTG TLSKFGCQMK FNLRRSFPLL TTKRVFWRGV VEELLWFISG
301: STNAKVLQEK GIHIWDGNAS REYLDGIGLT EREEGDLGPV YGFQWRHFGA KYTDMHADYT GQGFDQLVDV IDKIKNNPDD RRIIMSAWNP SDLKLMALPP
401: CHMFAQFYVA EGELSCQMYQ RSADMGLGVP FNIASYSLLT CMLAHVCDLV PGDFIHVLGD AHVYKTHVRP LQEQLLNLPK PFPVMKINPE KKQIDSFVAS
501: DFDLTGYDPH KKIEMKMAV

Arabidopsis Description

THY-1Bifunctional dihydrofolate reductase-thymidylate synthase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q05762]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.