CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 2
plastid 1
mitochondrion 2
cytosol 2
peroxisome 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH51504	Soybean	nucleus	92.83	86.62
CDX72487	Canola	cytosol	30.38	86.1
KRH24440	Soybean	cytosol, nucleus	82.26	82.26
KRH29421	Soybean	cytosol	81.89	82.2
Bra011543.1-P	Field mustard	cytosol	72.83	77.35
CDY45317	Canola	cytosol	72.26	77.06
Solyc01g109830.2.1	Tomato	cytosol	76.23	76.66
Bra017662.1-P	Field mustard	cytosol	72.83	76.59
PGSC0003DMT400001937	Potato	cytosol	75.85	76.28
CDX89705	Canola	cytosol	73.4	75.68
Os12t0446900-01	Rice	cytosol	74.15	75.43
AT2G16370.1	Thale cress	cytosol	73.77	75.34
Zm00001d007318_P001	Maize	cytosol	73.77	74.76
VIT_18s0075g01010.t01	Wine grape	cytosol, mitochondrion	12.26	74.71
Os11t0484400-01	Rice	cytosol, plasma membrane, plastid	73.4	74.66
CDX69070	Canola	mitochondrion, plastid	73.4	70.73
AT4G34570.1	Thale cress	mitochondrion	74.91	70.27
Zm00001d030396_P001	Maize	cytosol	37.74	70.18
Zm00001d050448_P001	Maize	cytosol	37.55	69.82
CDX75443	Canola	mitochondrion	73.4	68.37
KXG28376	Sorghum	mitochondrion, plastid	73.96	67.35
Zm00001d049188_P008	Maize	mitochondrion	73.58	67.01
TraesCS2A01G518600.1	Wheat	mitochondrion, plastid	72.26	66.49
TraesCS2D01G520000.1	Wheat	plastid	72.08	66.32
TraesCS2B01G547000.1	Wheat	plastid	72.08	66.2
HORVU2Hr1G114640.8	Barley	cytosol, plastid	71.7	62.71
GSMUA_Achr2P14300_001	Banana	cytosol	59.06	61.98
CDY02264	Canola	cytosol	71.7	61.49
Bra013079.1-P	Field mustard	cytosol	71.51	61.33
Bra000581.1-P	Field mustard	cytosol	49.25	57.74
CDX83194	Canola	cytosol	46.6	57.71
AT2G21550.1	Thale cress	cytosol, plasma membrane, plastid	52.83	56.91
CDY21464	Canola	cytosol	29.25	50.99
Os11t0615250-00	Rice	cytosol	8.3	39.64

Protein Annotations

KEGG:00240+2.1.1.45	KEGG:00670+1.5.1.3	KEGG:00670+1.5.1.3+2.1.1.45	KEGG:00670+2.1.1.45	KEGG:00790+1.5.1.3	EntrezGene:100786329
Gene3D:3.30.572.10	Gene3D:3.40.430.10	MapMan:6.3.1.2	MapMan:7.5.5	EMBL:ACUP02002140	InterPro:DHFR-TS
InterPro:DHFR-like_dom_sf	InterPro:DHFR_CS	InterPro:DHFR_dom	EnsemblPlantsGene:GLYMA_04G010800	GO:GO:0003674	GO:GO:0003824
GO:GO:0004146	GO:GO:0004799	GO:GO:0006139	GO:GO:0006231	GO:GO:0006545	GO:GO:0006730
GO:GO:0008150	GO:GO:0008152	GO:GO:0008168	GO:GO:0009058	GO:GO:0009165	GO:GO:0009987
GO:GO:0016491	GO:GO:0016740	GO:GO:0032259	GO:GO:0046654	GO:GO:0055114	InterPro:IPR001796
InterPro:IPR024072	InterPro:IPR036926	UniProt:K7KHI6	EnsemblPlants:KRH60811	ProteinID:KRH60811	ProteinID:KRH60811.1
ProteinID:KRH60812.1	HAMAP:MF_00008	PFAM:PF00186	PFAM:PF00303	PIRSF:PIRSF000389	PRINTS:PR00108
ScanProsite:PS00075	ScanProsite:PS00091	PFscan:PS51330	PANTHER:PTHR11548	PANTHER:PTHR11548:SF7	MetaCyc:PWY-3841
MetaCyc:PWY-6614	MetaCyc:PWY-7184	MetaCyc:PWY-7187	MetaCyc:PWY-7198	MetaCyc:PWY-7199	MetaCyc:PWY-7210
SUPFAM:SSF53597	SUPFAM:SSF55831	TIGRFAMs:TIGR03284	InterPro:Thymidate_synth/dCMP_Mease	InterPro:Thymidate_synth/dCMP_Mease_sf	InterPro:Thymidylate_synthase
InterPro:Thymidylate_synthase_AS	UniParc:UPI0002339F06	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr4:+:843870..849813

Molecular Weight (calculated)

59752.7 Da

IEP (calculated)

6.714

GRAVY (calculated)

-0.236

Length

530 amino acids

Sequence
(BLAST)

001: MAGDSSVMSN GYSKGSVNPL TNLRRTYQVL VAATQDWGIG KDGKLPWRLP TDLKFFKDIT VKTSDPGKKN AIVMGRKTWE SIPLEYRPLS GRLNVVLTRS
101: GSFDIATAEN VVICGSMSSA LELLAASPYS LSIEKVFVIG GGQIFREALN APGCEAIHLT EIQSSIECDT FMPPVDFTMF RPWYSSFPKV ENNIRYCFTT
201: YVRVRSSAPE SPGQNIDQLL DNNSESMKFE VKEFSFLPKM ISERHEEYLY LKLVQDIIAE GTTKDDRTGT GTLSKFGCQM RFNLHGSFPL LTTKRVFWRG
301: VVEELLWFIS GSTNAKVLQE KGIHIWDGNA SREYLDGVGL TEREEGDLGP VYGFQWRHFG ARYTDMHHDY SGQGFDQLLD VINKIKHNPD DRRIILSAWN
401: PSDLKLMALP PCHMFAQFYV AHGELSCQMY QRSADMGLGV PFNIASYALL TCMIAHVCDL IPGDFIHVIG DAHVYCNHVR PLQEQLHNQP KPFPTLEINP
501: KKKDIDSFVA ADFKLIGYDP HQKIDMKLAV

Best Arabidopsis Sequence Match ( AT2G16370.1 )
(BLAST)

001: MATTTLNDSV TTTLASEPQR TYQVVVAATK EMGIGKDGKL PWNLPTDLKF FKDITLTTSD SSKKNAVVMG RKTWESIPIK YRPLSGRLNV VLTRSGGFDI
101: ANTENVVTCS SVDSALDLLA APPYCLSIER VFVIGGGDIL REALNRPSCD AIHLTEIDTS VDCDTFIPAI DTSVYQPWSS SFPVTENGLR FCFTTFVRVK
201: SSADESSDES NGSQSLQFDG KKFLFLPKMV FDQHEEFLYL NMVEDIISNG NVKNDRTGTG TLSKFGCQMK FNLRRSFPLL TTKRVFWRGV VEELLWFISG
301: STNAKVLQEK GIHIWDGNAS REYLDGIGLT EREEGDLGPV YGFQWRHFGA KYTDMHADYT GQGFDQLVDV IDKIKNNPDD RRIIMSAWNP SDLKLMALPP
401: CHMFAQFYVA EGELSCQMYQ RSADMGLGVP FNIASYSLLT CMLAHVCDLV PGDFIHVLGD AHVYKTHVRP LQEQLLNLPK PFPVMKINPE KKQIDSFVAS
501: DFDLTGYDPH KKIEMKMAV

Arabidopsis Description

THY-1Bifunctional dihydrofolate reductase-thymidylate synthase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q05762]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.