CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 9
plasma membrane 2
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH60811	Soybean	nucleus	86.62	92.83
CDX72487	Canola	cytosol	27.99	85.03
KRH29421	Soybean	cytosol	76.41	82.2
KRH24440	Soybean	cytosol, nucleus	76.23	81.7
Bra011543.1-P	Field mustard	cytosol	67.96	77.35
Solyc01g109830.2.1	Tomato	cytosol	71.13	76.66
Zm00001d007318_P001	Maize	cytosol	70.07	76.1
PGSC0003DMT400001937	Potato	cytosol	70.6	76.09
CDY45317	Canola	cytosol	66.2	75.65
Bra017662.1-P	Field mustard	cytosol	67.08	75.6
CDX89705	Canola	cytosol	68.31	75.49
Os12t0446900-01	Rice	cytosol	69.19	75.43
Os11t0484400-01	Rice	cytosol, plasma membrane, plastid	69.19	75.43
AT2G16370.1	Thale cress	cytosol	68.13	74.57
VIT_18s0075g01010.t01	Wine grape	cytosol, mitochondrion	11.27	73.56
CDX69070	Canola	mitochondrion, plastid	69.37	71.64
Zm00001d030396_P001	Maize	cytosol	35.74	71.23
Zm00001d050448_P001	Maize	cytosol	35.39	70.53
AT4G34570.1	Thale cress	mitochondrion	70.07	70.44
CDX75443	Canola	mitochondrion	68.84	68.72
KXG28376	Sorghum	mitochondrion, plastid	69.89	68.21
Zm00001d049188_P008	Maize	mitochondrion	69.72	68.04
TraesCS2B01G547000.1	Wheat	plastid	68.31	67.24
TraesCS2A01G518600.1	Wheat	mitochondrion, plastid	68.13	67.19
TraesCS2D01G520000.1	Wheat	plastid	67.96	67.01
HORVU2Hr1G114640.8	Barley	cytosol, plastid	67.96	63.7
GSMUA_Achr2P14300_001	Banana	cytosol	55.46	62.38
CDY02264	Canola	cytosol	66.37	61.0
Bra013079.1-P	Field mustard	cytosol	66.37	61.0
CDX83194	Canola	cytosol	43.31	57.48
AT2G21550.1	Thale cress	cytosol, plasma membrane, plastid	49.65	57.32
Bra000581.1-P	Field mustard	cytosol	45.6	57.3
CDY21464	Canola	cytosol	27.29	50.99
Os11t0615250-00	Rice	cytosol	7.75	39.64

Protein Annotations

KEGG:00240+2.1.1.45	KEGG:00670+1.5.1.3	KEGG:00670+1.5.1.3+2.1.1.45	KEGG:00670+2.1.1.45	KEGG:00790+1.5.1.3	Gene3D:3.30.572.10
Gene3D:3.40.430.10	EntrezGene:547833	MapMan:6.3.1.2	MapMan:7.5.5	EMBL:ACUP02003379	InterPro:DHFR-TS
InterPro:DHFR-like_dom_sf	InterPro:DHFR_CS	InterPro:DHFR_dom	EnsemblPlantsGene:GLYMA_06G010600	GO:GO:0003674	GO:GO:0003824
GO:GO:0004146	GO:GO:0004799	GO:GO:0006139	GO:GO:0006231	GO:GO:0006545	GO:GO:0006730
GO:GO:0008150	GO:GO:0008152	GO:GO:0008168	GO:GO:0009058	GO:GO:0009165	GO:GO:0009987
GO:GO:0016491	GO:GO:0016740	GO:GO:0032259	GO:GO:0046654	GO:GO:0055114	UniProt:I1K754
InterPro:IPR001796	InterPro:IPR024072	InterPro:IPR036926	EnsemblPlants:KRH51504	ProteinID:KRH51504	ProteinID:KRH51504.1
HAMAP:MF_00008	PFAM:PF00186	PFAM:PF00303	PIRSF:PIRSF000389	PRINTS:PR00108	ScanProsite:PS00075
ScanProsite:PS00091	PFscan:PS51330	PANTHER:PTHR11548	PANTHER:PTHR11548:SF7	MetaCyc:PWY-3841	MetaCyc:PWY-6614
MetaCyc:PWY-7184	MetaCyc:PWY-7187	MetaCyc:PWY-7198	MetaCyc:PWY-7199	MetaCyc:PWY-7210	SUPFAM:SSF53597
SUPFAM:SSF55831	SignalP:SignalP-noTM	TIGRFAMs:TIGR03284	InterPro:Thymidate_synth/dCMP_Mease	InterPro:Thymidate_synth/dCMP_Mease_sf	InterPro:Thymidylate_synthase
InterPro:Thymidylate_synthase_AS	UniParc:UPI0002952B10	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr6:+:828070..833094

Molecular Weight (calculated)

64166.2 Da

IEP (calculated)

7.719

GRAVY (calculated)

-0.192

Length

568 amino acids

Sequence
(BLAST)

001: MFSFCSFLTI SLHFPKNWLC SPCFQTPSLS YCRRLSSIMP SDSSVISNGH SNGSVNPLPN LQRTYQVVVA ATQDWGIGKD GKLPWRLPTD LKFFKEITMK
101: TSDPGKKNAI VMGRKTWESI PLEYRPLSGR LNVVLTRSGS FDIATAENVV ICGSMSSALE LLAASPYSLS IEKVFVIGGG QIFREALNVP GCEAIHLTEI
201: QSSIECDTFM PPVDFTIFRP WYSSFPKVEN NIRYSFTTYV RVRSSAAESA GQNIDPLLDN NSESMKFEVK DFSFLPKMIS ERHEEYLYLK LVQDIIAEGT
301: TKGDRTGTGT LSKFGCQMRF NLRGNFPLLT TKKVFWRGVV EELLWFISGS TNAKVLQEKG IHIWDGNASR EYLDGVGLTE REEGDLGPVY GFQWRHFGAR
401: YTDMHHDYSG QGFDQLLDVI NKIKRNPDDR RIILSAWNPV DLKLMALPPC HMFAQFYVAH GELSCQMYQR SADMGLGIPF NIASYALLTC MIAHVCDLIP
501: GDFIHVIGDA HIYRNHVRPL QEQLHNQPKP FPTLKINPKK KDIDSFVAAD FKLIGYDPHQ KIDMKLSV

Best Arabidopsis Sequence Match ( AT2G16370.1 )
(BLAST)

001: MATTTLNDSV TTTLASEPQR TYQVVVAATK EMGIGKDGKL PWNLPTDLKF FKDITLTTSD SSKKNAVVMG RKTWESIPIK YRPLSGRLNV VLTRSGGFDI
101: ANTENVVTCS SVDSALDLLA APPYCLSIER VFVIGGGDIL REALNRPSCD AIHLTEIDTS VDCDTFIPAI DTSVYQPWSS SFPVTENGLR FCFTTFVRVK
201: SSADESSDES NGSQSLQFDG KKFLFLPKMV FDQHEEFLYL NMVEDIISNG NVKNDRTGTG TLSKFGCQMK FNLRRSFPLL TTKRVFWRGV VEELLWFISG
301: STNAKVLQEK GIHIWDGNAS REYLDGIGLT EREEGDLGPV YGFQWRHFGA KYTDMHADYT GQGFDQLVDV IDKIKNNPDD RRIIMSAWNP SDLKLMALPP
401: CHMFAQFYVA EGELSCQMYQ RSADMGLGVP FNIASYSLLT CMLAHVCDLV PGDFIHVLGD AHVYKTHVRP LQEQLLNLPK PFPVMKINPE KKQIDSFVAS
501: DFDLTGYDPH KKIEMKMAV

Arabidopsis Description

THY-1Bifunctional dihydrofolate reductase-thymidylate synthase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q05762]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.