CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

cytosol 3
plastid 2
mitochondrion 2
peroxisome 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH10294
KRH11018
KRH21268
KRH23029
KRH24840
KRH29844
KRH56371
KRH62568

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G22780.1	KRH11018	AT1G29260.1	24879895
AT2G22780.1	KRH21268	AT1G29260.1	24879895
AT2G22780.1	KRH10294	AT3G46090.1	17259181
AT2G22780.1	KRH23029	AT3G46090.1	17259181
AT2G22780.1	KRH24840	AT3G46090.1	17259181
AT2G22780.1	KRH29844	AT3G46090.1	17259181
AT2G22780.1	KRH56371	AT5G56290.1	24879895
AT2G22780.1	KRH62568	AT5G56290.1	24879895

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH77189	Soybean	endoplasmic reticulum	72.8	88.93
Solyc02g063490.2.1	Tomato	plastid	83.57	83.81
PGSC0003DMT400026930	Potato	plastid	83.57	83.81
KRH48807	Soybean	mitochondrion	34.84	83.11
Os12t0632700-01	Rice	extracellular, nucleus, plastid	82.15	81.46
TraesCS5A01G014300.1	Wheat	nucleus, plastid	81.59	80.45
GSMUA_Achr4P01380_001	Banana	plasma membrane	81.02	80.34
KRH42019	Soybean	nucleus	81.02	80.34
CDY25631	Canola	plastid	80.17	80.17
KRH49884	Soybean	endoplasmic reticulum, nucleus	80.74	80.06
Zm00001d041243_P001	Maize	mitochondrion	81.59	80.0
AT2G22780.1	Thale cress	cytosol	80.17	79.94
TraesCS5D01G019700.1	Wheat	nucleus	81.02	79.89
Bra030204.1-P	Field mustard	cytosol	79.89	79.89
TraesCS5B01G012400.1	Wheat	cytosol	80.74	79.61
CDX89514	Canola	cytosol	79.6	79.6
HORVU5Hr1G002010.1	Barley	plastid	81.3	70.17
VIT_00s0373g00040.t01	Wine grape	peroxisome	85.84	69.82
CDY43430	Canola	cytosol	33.43	68.21
CDY17513	Canola	cytosol	30.03	67.95
KXG24112	Sorghum	plastid	81.87	64.8
KRH26205	Soybean	mitochondrion	62.32	63.77
KRH55117	Soybean	mitochondrion	62.04	63.48
KRH03482	Soybean	endoplasmic reticulum, mitochondrion, nucleus, plastid	60.34	52.08
KRH56907	Soybean	endoplasmic reticulum	60.91	52.06
CDY34030	Canola	mitochondrion	32.01	40.5

Protein Annotations

KEGG:00020+1.1.1.37	KEGG:00270+1.1.1.37	KEGG:00620+1.1.1.37	KEGG:00630+1.1.1.37	KEGG:00680+1.1.1.37	KEGG:00710+1.1.1.37
KEGG:00720+1.1.1.37	Gene3D:3.40.50.720	Gene3D:3.90.110.10	MapMan:5.7.3.6.5	EntrezGene:732641	EMBL:ACUP02006743
EnsemblPlantsGene:GLYMA_11G043900	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005777	GO:GO:0005975	GO:GO:0006091	GO:GO:0006097	GO:GO:0006099
GO:GO:0006108	GO:GO:0008150	GO:GO:0008152	GO:GO:0009514	GO:GO:0009987	GO:GO:0016491
GO:GO:0016615	GO:GO:0016616	GO:GO:0019752	GO:GO:0030060	GO:GO:0055114	UniProt:I1LH25
InterPro:IPR015955	EnsemblPlants:KRH28298	ProteinID:KRH28298	ProteinID:KRH28298.1	InterPro:L-lactate/malate_DH	InterPro:Lactate/malate_DH_C
InterPro:Lactate/malate_DH_N	InterPro:Lactate_DH/Glyco_Ohase_4_C	InterPro:Malate_DH_AS	InterPro:Malate_DH_type1	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00056
PFAM:PF02866	PIRSF:PIRSF000102	ScanProsite:PS00068	PANTHER:PTHR11540	PANTHER:PTHR11540:SF34	MetaCyc:PWY-1622
MetaCyc:PWY-5392	MetaCyc:PWY-561	MetaCyc:PWY-5690	MetaCyc:PWY-5913	MetaCyc:PWY-6728	MetaCyc:PWY-6969
MetaCyc:PWY-7115	MetaCyc:PWY-7383	SUPFAM:SSF51735	SUPFAM:SSF56327	TIGRFAMs:TIGR01772	TMHMM:TMhelix
UniParc:UPI000233D164	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr11:-:3252904..3256995

Molecular Weight (calculated)

37400.8 Da

IEP (calculated)

7.718

GRAVY (calculated)

0.171

Length

353 amino acids

Sequence
(BLAST)

001: MEANSGASDR ISRIAGHLRP QREDDVCLKR SDCRAKGGVS GFKVAILGAA GGIGQPLAML MKMNPLVSLL HLYDVVNTPG VTSDISHMDT GAVVRGFLGQ
101: QQLEDALIGM DLVIIPAGVP RKPGMTRDDL FNINAGIVKT LCEAIAKCCP KAIVNVISNP VNSTVPIAAE VFKRAGTYDP KRLLGVTMLD VVRANTFVAE
201: VLGVDPRDVD VPVVGGHAGI TILPLLSQIK PPCSFTPKEI EYLTGRIQNG GTEVVEAKAG AGSATLSMAY AAVKFADACL HALRGDAGII ECAYVASQVT
301: ELPFFASKVR LGRVGVEEIL PLGPLNDYER ESLEKAKKEL AASIEKGISF IRK

Best Arabidopsis Sequence Match ( AT2G22780.1 )
(BLAST)

001: MDPNQRIARI SAHLNPPNLH NQIADGSGLN RVACRAKGGS PGFKVAILGA AGGIGQPLAM LMKMNPLVSV LHLYDVANAP GVTADISHMD TSAVVRGFLG
101: QPQLEEALTG MDLVIIPAGV PRKPGMTRDD LFNINAGIVR TLSEAIAKCC PKAIVNIISN PVNSTVPIAA EVFKKAGTFD PKKLMGVTML DVVRANTFVA
201: EVMSLDPREV EVPVVGGHAG VTILPLLSQV KPPCSFTQKE IEYLTDRIQN GGTEVVEAKA GAGSATLSMA YAAVEFADAC LRGLRGDANI VECAYVASHV
301: TELPFFASKV RLGRCGIDEV YGLGPLNEYE RMGLEKAKKE LSVSIHKGVT FAKK

Arabidopsis Description

PMDH1Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc:Q0WUS4]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.