CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 4
cytosol 1
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
PGSC0003DMT400030470	Potato	cytosol	4.02	84.38
PGSC0003DMT400029834	Potato	cytosol	3.94	82.81
PGSC0003DMT400071616	Potato	cytosol	36.68	70.33
Solyc03g058380.2.1	Tomato	nucleus	39.66	70.04
AT2G27700.2	Thale cress	cytosol	24.78	69.38
PGSC0003DMT400033284	Potato	cytosol	7.22	67.83
GSMUA_Achr5P09710_001	Banana	cytosol	44.2	66.22
CDY49760	Canola	plastid	21.21	65.37
KRG91641	Soybean	nucleus	65.77	65.24
CDY45947	Canola	nucleus	49.03	57.45
Solyc06g054400.2.1	Tomato	nucleus	47.77	56.27
CDY33673	Canola	cytosol	41.29	55.06
CDX96600	Canola	cytosol	40.62	54.55
Bra016430.1-P	Field mustard	cytosol	41.74	54.2
CDY20385	Canola	nucleus	48.96	54.07
Bra008279.1-P	Field mustard	nucleus	50.89	53.94
AT1G76810.1	Thale cress	nucleus	51.49	53.48
Os05t0592600-01	Rice	nucleus	47.54	52.94
VIT_18s0076g00310.t01	Wine grape	nucleus	52.53	52.22
KRG88874	Soybean	cytosol	15.03	52.2
TraesCS7B01G382100.1	Wheat	cytosol	20.01	51.83
KXG22680	Sorghum	nucleus	48.74	50.66
Solyc03g058350.2.1	Tomato	nucleus	43.53	50.61
Zm00001d009749_P001	Maize	nucleus	48.51	50.43
TraesCS1A01G438600.2	Wheat	nucleus	48.07	50.04
AT1G76720.2	Thale cress	cytosol	45.54	50.0
HORVU1Hr1G094740.1	Barley	nucleus	47.62	49.65
TraesCS1D01G446400.1	Wheat	golgi	47.84	49.61
TraesCS1B01G472500.1	Wheat	nucleus	47.84	49.46
PGSC0003DMT400013995	Potato	nucleus	47.62	49.46
AT1G21160.1	Thale cress	cytosol	40.03	49.27
PGSC0003DMT400079119	Potato	nucleus	48.51	48.77
PGSC0003DMT400011181	Potato	cytosol, mitochondrion	9.67	47.1
TraesCS1D01G446900.2	Wheat	nucleus	47.25	45.23
OQU79180	Sorghum	nucleus	27.68	43.31
TraesCS7A01G479800.2	Wheat	mitochondrion, plastid	25.45	41.25
CDX81961	Canola	cytosol	7.96	36.27
CDX81960	Canola	cytosol	7.29	35.13
CDY13882	Canola	cytosol	7.22	32.99
Bra031112.1-P	Field mustard	cytosol	7.22	32.99
GSMUA_Achr1P05220_001	Banana	nucleus	44.35	32.84
CDY13881	Canola	cytosol	9.23	27.31
Bra031113.1-P	Field mustard	cytosol	9.15	27.09
KRH48578	Soybean	cytosol, extracellular	1.79	21.05
KRH68380	Soybean	mitochondrion	8.93	16.71
KRG96646	Soybean	mitochondrion	8.93	16.71
KRH13635	Soybean	nucleus	11.16	14.78
KRH43837	Soybean	nucleus, plastid	11.09	14.75

Protein Annotations

EntrezGene:100814640	MapMan:17.4.3.2	Gene3D:2.40.30.10	Gene3D:3.40.50.10050	Gene3D:3.40.50.300	EMBL:ACUP02006653
ncoils:Coil	InterPro:EFTu-like_2	EnsemblPlantsGene:GLYMA_10G225800	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0003924	GO:GO:0005488	GO:GO:0005525	GO:GO:0016787	UniProt:I1LDH2	InterPro:IPR000795
InterPro:IPR036925	EnsemblPlants:KRH35157	ProteinID:KRH35157	ProteinID:KRH35157.1	InterPro:P-loop_NTPase	PFAM:PF00009
PFAM:PF03144	PFAM:PF11987	PRINTS:PR00315	PFscan:PS51722	PANTHER:PTHR43381	PANTHER:PTHR43381:SF4
SUPFAM:SSF50447	SUPFAM:SSF52156	SUPFAM:SSF52540	InterPro:Small_GTP-bd_dom	InterPro:TF_GTP-bd_dom	InterPro:TIF_IF2
InterPro:TIF_IF2_dom3	InterPro:TIF_IF2_dom3_sf	TIGRFAMs:TIGR00231	InterPro:Transl_B-barrel_sf	UniParc:UPI000233C4E8	SEG:seg

Description

hypothetical protein

Coordinates

chr10:+:45629065..45639027

Molecular Weight (calculated)

147080.0 Da

IEP (calculated)

4.941

GRAVY (calculated)

-0.773

Length

1344 amino acids

Sequence
(BLAST)

0001: MGKKKPTARD EENASQQGGG GKSKKKAVVI DDDEYSVGTE LSEEQAPASE EKPAAAGKKK GKKGNAKAKN NDDDVDEDED VSGIVFAGKK KGKNKKGGGS
0101: SAFTASGFDL LGDEEDDDEE KSGLTGEVDG EEEDEPVVSF TGKKKSSKKG GGSLFSASAF DDGEEEEDET VISFSGKKKS SRGSKNSASA FDAIDDDADG
0201: KVVDDKNDDV DDDDEPVISF TGKKKSSKGG KKGGSVFSAA VLGEIDDDEE NKDDGGGDDD DDIGPITFSG KKRKSSKKAA NSVSKGASVE EGDDKDEDDV
0301: SLVAFSGKKK SSKKKGSSAA AKASDENVDV VEPEAPSVGS TDAGNSNVNK SEEVAGNSKN KKKNKKKSGR TAQEEEDLDK LLAELGETPP VPKPTTLPQD
0401: DKVQPIPEVV PVADASGQKE GEEETVETAA AKKKKKKKEK EKEKKAAAAA SAVGTAPENG TAEDKAEVIE PKKNDSKAKK AADKKVPKHV REMQEALARR
0501: QEAEERKKRE EEERLRKEEE ERRKQEELER QAEEARRRKK EREKEKLQKK KQEGKLLTGK QKEEARRLEA MRRQILNNTG GMTLPGGDSG APPKKPIYQT
0601: KKVKPNNRNQ NGAAAAAPAQ TAETVEAKET DADLASEEPE KIEEVESVQV DDKVELLVAD EDDGAEDDDE DEWDAKSWDD VNLNNKGAFA DEEVDSEPKP
0701: IVKEIKNAVP AQNAGATKPV VEEIENGKQA KPHLNREPRK SAVPPKPSDE NLRSPICCIM GHVDTGKTKL LDCIRGTNVQ EGEAGGITQQ IGATYFPAEN
0801: IRERTKELKA DAKLKVPGLL VIDTPGHESF TNLRSRGSGL CDIAILVVDI MHGLEQQTIE SLNLLKMRNT EFIVALNKVD RLYGWKTCRN APIVKAMKQQ
0901: TKDVQNEFNM RLTQIITEFK EQGLNTELYY KNKEMGETFS IVPTSAISGE GIPDLLLLLI QWTQKTMVEK LTYSEEVQCT VLEVKVVEGH GTTIDVVLVN
1001: GVLHEGEQIV VCGMQGPIVT TIRALLTPHP MKELRVKGTY LHHKEIKAAM GIKITAQGLE HAIAGTGLYV VKPDDDLEDV KESAMEDMRS VMSRIDRTGE
1101: GVCVQASTLG SLEALLEFLK TPEVSIPVSG ISIGPVHKKD VMKASVMLEK KREYAAILAF DVKVTPEARE LADELGVKIF IADIIYHLFD QFKAYIDNIK
1201: EEKKREAADE AVFPCVMSIL PNCIFNKKDP IVLGVDILEG ILKIGTPICI PSREFIDIGR IASIENNHKP VDYAKKGQKV AIKIVGSNSE EQQKMFGRHF
1301: EIDDELVSHI SRRSIDILKT NYRDELNMEE WRLVVKLKNL FKIQ

Best Arabidopsis Sequence Match ( AT1G76810.1 )
(BLAST)

0001: MGRKKPSARG GDAEQQPPAS SLVGATKSKK KGAQIDDDEY SIGTELSEES KVEEEKVVVI TGKKKGKKGN KKGTQQDDDD DFSDKVSAAG VKDDVPEIAF
0101: VGKKKSKGKK GGGSVSFALL DDEDEKEDNE SDGDKDDEPV ISFTGKKHAS KKGKKGGNSF AASAFDALGS DDDDTEEVHE DEEEESPITF SGKKKKSSKS
0201: SKKNTNSFTA DLLDEEEGTD ASNSRDDENT IEDEESPEVT FSGKKKSSKK KGGSVLASVG DDSVADETKT SDTKNVEVVE TGKSKKKKKN NKSGRTVQEE
0301: EDLDKLLAAL GETPAAERPA SSTPVEEKAA QPEPVAPVEN AGEKEGEEET AAAKKKKKKK EKEKEKKAAA AAAATSSVEV KEEKQEESVT EPLQPKKKDA
0401: KGKAAEKKIP KHVREMQEAL ARRQEAEERK KKEEEEKLRK EEEERRRQEE LEAQAEEAKR KRKEKEKEKL LRKKLEGKLL TAKQKTEAQK REAFKNQLLA
0501: AGGGLPVADN DGDATSSKRP IYANKKKSSR QKGIDTSVQG EDEVEPKENQ ADEQDTLGEV GLTDTGKVDL IELVNTDENS GPADVAQENG VEEDDEEDEW
0601: DAKSWGTVDL NLKGDFDDEE EEAQPVVKKE LKDAISKAHD SEPEAEKPTA KPAGTGKPLI AAVKATPEVE DATRTKRATR AKDASKKGKG LAPSESIEGE
0701: ENLRSPICCI MGHVDTGKTK LLDCIRGTNV QEGEAGGITQ QIGATYFPAE NIRERTKELK ADAKLKVPGL LVIDTPGHES FTNLRSRGSS LCDLAILVVD
0801: IMHGLEPQTI ESLNLLRMRN TEFIVALNKV DRLYGWKTCK NAPIVKAMKQ QNKDVINEFN LRLKNIINEF QEQGLNTELY YKNKDMGDTF SIVPTSAISG
0901: EGVPDLLLWL VQWAQKTMVE KLTYVDEVQC TVLEVKVIEG HGTTIDVVLV NGELHEGDQI VVCGLQGPIV TTIRALLTPH PMKELRVKGT YLHYKEIKAA
1001: QGIKITAQGL EHAIAGTALH VVGPDDDIEA IKESAMEDME SVLSRIDKSG EGVYVQASTL GSLEALLEYL KSPAVKIPVS GIGIGPVHKK DVMKAGVMLE
1101: RKKEYATILA FDVKVTTEAR ELADEMGVKI FCADIIYHLF DLFKAYIENI KEEKKKESAD EAVFPCVLQI LPNCVFNKKD PIVLGVDVIE GILKIGTPIC
1201: VPGREFIDIG RIASIENNHK PVDYAKKGNK VAIKIVGSNA EEQKMFGRHF DMEDELVSHI SRRSIDILKS NYRDELSLEE WKLVVKLKNI FKIQ

Arabidopsis Description

Eukaryotic translation initiation factor 2 (eIF-2) family protein [Source:UniProtKB/TrEMBL;Acc:F4I420]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.