CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 6
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG93223
KRG95876
KRG95883
KRG96443
KRH01744
KRH02172
KRH13056
KRH15102
KRH38014
KRH42195
KRH46935
KRH50944
KRH58317
KRH67593
KRH67596
KRH68188
KRH73034

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G64200.2	KRG96443	AT1G02840.1	18674533
AT5G64200.2	KRH68188	AT1G02840.1	18674533
AT5G64200.2	KRG93223	AT1G53720.1	16497658
AT5G64200.2	KRH13056	AT1G53720.1	16497658
AT5G64200.2	KRG95876	AT2G37340.1	18674533
AT5G64200.2	KRG95883	AT2G37340.1	18674533
AT5G64200.2	KRH42195	AT2G37340.1	18674533
AT5G64200.2	KRH58317	AT2G37340.1	18674533
AT5G64200.2	KRH67593	AT2G37340.1	18674533
AT5G64200.2	KRH67596	AT2G37340.1	18674533
AT5G64200.2	KRH01744	AT2G43370.1	15987817
AT5G64200.2	KRH46935	AT2G43370.1	15987817
AT5G64200.2	KRH38014	AT3G13570.1	18674533
AT5G64200.2	KRH15102	AT3G55460.1	18674533
AT5G64200.2	KRH73034	AT3G55460.1	18674533
AT5G64200.2	KRH02172	AT5G18810.1	18674533
AT5G64200.2	KRH50944	AT5G18810.1	18674533

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH58277	Soybean	nucleus	98.13	98.13
HORVU2Hr1G029640.1	Barley	mitochondrion	40.45	77.7
VIT_12s0142g00110.t01	Wine grape	nucleus	73.41	76.86
Solyc01g105140.2.1	Tomato	nucleus	70.41	74.02
PGSC0003DMT400032709	Potato	nucleus	69.66	73.23
GSMUA_Achr10P...	Banana	nucleus	64.42	72.57
KXG36736	Sorghum	nucleus	65.54	70.0
Zm00001d006906_P010	Maize	mitochondrion	64.04	67.86
Bra024276.1-P	Field mustard	nucleus	70.79	67.5
Zm00001d022189_P002	Maize	nucleus	61.42	64.57
TraesCS2D01G169700.5	Wheat	nucleus	66.29	64.36
Os07t0623300-01	Rice	nucleus	64.04	62.18
AT5G64200.2	Thale cress	nucleus	68.54	60.4
KXG39239	Sorghum	nucleus	53.18	60.17
TraesCS2A01G162700.3	Wheat	nucleus	52.81	59.24
TraesCS2B01G188600.1	Wheat	nucleus, plastid	59.93	58.82
HORVU2Hr1G029630.1	Barley	plastid	26.22	51.47
CDX84503	Canola	nucleus	50.19	49.45
KRH16958	Soybean	nucleus	31.84	34.14
KRH72576	Soybean	nucleus	31.09	32.81
KRH02172	Soybean	nucleus	29.96	32.0
KRH50944	Soybean	nucleus	28.46	31.28
CDY43799	Canola	vacuole	37.08	30.65
KRH15102	Soybean	nucleus	29.21	28.78
KRH73034	Soybean	nucleus	29.59	28.62
Zm00001d029380_P002	Maize	cytosol, nucleus, plasma membrane	38.2	23.61
KRH38014	Soybean	endoplasmic reticulum, nucleus	24.34	22.73

Protein Annotations

EntrezGene:100778928	Gene3D:3.30.70.330	MapMan:35.1	EMBL:ACUP02004791	EnsemblPlantsGene:GLYMA_08G072600	GO:GO:0003674
GO:GO:0003676	GO:GO:0003723	GO:GO:0005488	UniProt:I1KR32	InterPro:IPR000504	InterPro:IPR012677
EnsemblPlants:KRH42160	ProteinID:KRH42160	ProteinID:KRH42160.1	ProteinID:KRH42161.1	InterPro:Nucleotide-bd_a/b_plait_sf	PFAM:PF00076
PFscan:PS50102	PANTHER:PTHR23147	PANTHER:PTHR23147:SF42	InterPro:RBD_domain_sf	InterPro:RRM_dom	InterPro:RRM_dom_euk
SMART:SM00360	SMART:SM00361	SUPFAM:SSF54928	UniParc:UPI000233855C	SEG:seg	:

Description

hypothetical protein

Coordinates

chr8:+:5564568..5568855

Molecular Weight (calculated)

31040.9 Da

IEP (calculated)

11.876

GRAVY (calculated)

-1.612

Length

267 amino acids

Sequence
(BLAST)

001: MSHFGRSGPP DISDTYSLLV LNITFRTTAD DLFPLFDKYG KVVDIFIPKD RRTGESRGFA FVRYKYADEA QKAVERLDGR MVDGREITVQ FAKYGPNAER
101: IHKGRIIETS PRSRRSRSRS PRKRHRDDYK DRDYRRRSRS RSYDRYERDR HRGRDKDYRR RSRSRSASLD YKGRGRGRYD DERNSRSRSR SVDSGSPARR
201: SPSPRRSPSP QRSTSPQRSP SPRKSPRGES PANRSRDGRS PTPRSVSPRG RPDASRSPSP RNSNGDE

Best Arabidopsis Sequence Match ( AT5G64200.1 )
(BLAST)

001: MSHFGRSGPP DISDTYSLLV LNITFRTTAD DLYPLFAKYG KVVDVFIPRD RRTGDSRGFA FVRYKYKDEA HKAVERLDGR VVDGREITVQ FAKYGPNAEK
101: ISKGRVVEPP PKSRRSRSRS PRRSRSPRRS RSPPRRRSPR RSRSPRRRSR DDYREKDYRK RSRSRSYDRR ERHEEKDRDH RRRTRSRSAS PDEKRRVRGR
201: YDNESRSHSR SLSASPARRS PRSSSPQKTS PAREVSPDKR SNERSPSPRR SLSPRSPALQ KASPSKEMSP ERRSNERSPS PGSPAPLRKV DAASRSQSPY
301: AAE

Arabidopsis Description

SC35Serine/arginine-rich splicing factor SC35 [Source:UniProtKB/Swiss-Prot;Acc:Q9FMG4]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.