CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

extracellular 5
endoplasmic reticulum 6
vacuole 7
plasma membrane 5
golgi 5

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	vacuole
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	vacuole
YLoc:	endoplasmic reticulum

cytosol: 16847873

msms PMID: 16847873 doi

V Katavic, GK Agrawal, M Hajduch, SL Harris, JJ Thelen

University of Missouri-Columbia, Department of Biochemistry, Columbia 65211, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
CDY32970
CDY43592

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G26000.1	CDY32970	AT5G42980.1	15352244
AT5G26000.1	CDY43592	AT5G42980.1	15352244

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY24996	Canola	cytosol	31.88	95.45
CDY67046	Canola	extracellular	95.64	92.31
CDY68654	Canola	cytosol	31.5	92.22
CDY26308	Canola	cytosol	94.12	91.01
CDY24995	Canola	cytosol, plastid, vacuole	55.6	86.94
Bra020549.1-P	Field mustard	cytosol	40.42	81.3
CDY70871	Canola	mitochondrion, vacuole	34.91	80.7
CDY37226	Canola	extracellular	79.89	76.13
CDY71022	Canola	cytosol	74.95	73.83
CDY65716	Canola	cytosol	18.98	73.53
CDY04261	Canola	vacuole	52.94	73.23
CDY72290	Canola	cytosol, mitochondrion, vacuole	25.81	73.12
CDY52412	Canola	cytosol	12.33	73.03
CDY24999	Canola	cytosol, mitochondrion	28.08	72.91
CDY52022	Canola	cytosol	72.87	72.32
CDY07361	Canola	endoplasmic reticulum	73.24	71.75
CDY09042	Canola	vacuole	69.83	69.7
CDY22724	Canola	cytosol	70.21	67.77
CDY22731	Canola	cytosol	22.58	67.61
AT5G26000.1	Thale cress	endoplasmic reticulum, vacuole	68.69	66.91
CDY39513	Canola	endoplasmic reticulum, vacuole	66.22	66.35
CDY46331	Canola	endoplasmic reticulum, vacuole	66.22	64.39
CDY09054	Canola	vacuole	58.63	62.8
CDY29614	Canola	cytosol, peroxisome, vacuole	10.63	60.22
CDY71771	Canola	cytosol, vacuole	39.09	56.44
CDX90772	Canola	mitochondrion	59.96	53.38
CDY46560	Canola	cytosol, vacuole	8.54	45.0
CDY22730	Canola	mitochondrion	37.57	43.81
CDX87822	Canola	plasma membrane	8.92	37.3
CDY29604	Canola	mitochondrion, plastid, vacuole	11.76	36.9
CDY67044	Canola	cytosol	0.76	23.53

Protein Annotations

Gene3D:3.20.20.80	MapMan:9.5.2.1	GO:A0A078EXM1	EnsemblPlants:CDY05334	ProteinID:CDY05334	ProteinID:CDY05334.1
GO:GO:0003674	GO:GO:0003824	GO:GO:0004553	GO:GO:0005975	GO:GO:0008150	GO:GO:0008152
GO:GO:0016787	EnsemblPlantsGene:GSBRNA2T00120182001	InterPro:Glyco_hydro_1	InterPro:Glyco_hydro_1_AS	InterPro:Glycoside_hydrolase_SF	PFAM:PF00232
PRINTS:PR00131	ScanProsite:PS00572	PFscan:PS51257	PANTHER:PTHR10353	PANTHER:PTHR10353:SF44	SUPFAM:SSF51445
SignalP:SignalP-noTM	UniParc:UPI0004EEAC02	SEG:seg	:	:	:

Description

BnaC02g40420D

Coordinates

chrLK031961:+:262406..265315

Molecular Weight (calculated)

60357.1 Da

IEP (calculated)

6.304

GRAVY (calculated)

-0.491

Length

527 amino acids

Sequence
(BLAST)

001: MKLLHGLALV FLLAAASCKA DEEITCEENN PFTCSNTDIL SSKNFGKDFI FGIEGGRGRG VNVWDGFSHR YPEKSGSDLK NGDTTCESYT RWQKDVDVMG
101: ELNATGYRFS FAWSRIIPKG KVSRGVNQGG LDYYHKLIDA LLEKNITPFV TLFHWDLPQT LQDEYEGFLD RQIIQDFKDY ADLCFKEFGG KVKHWITINQ
201: LYTVPTRGYA VGTDAPEPYI VAHNQLLAHA TVADHYRKNY AVSSLDIAKK GKIGPVMITR WFLPYDESDP ASIEAAERMN QFFHGWYMEP LTKGRYPDIM
301: RQIVGSRLPN FTEEEAELVA GSYDFLGLNY YVTQYAQPKP NPYPSETHTA MMDAGVKLTY DNSRGEFLGP LFVEDEVNGN SYYYPKGIYY VMDYFKTKYG
401: DPLIYVTENG FSTPSSENRE QAIADYKRID YLCSHLCFLR KVIKEKGVNV RGYFAWALGD NYEFCKGFTV RFGLSYVNWE DLDDRNLKES GKWYQRFING
501: TVKNSAKQDF LRSSLSSQSQ KKRLADA

Best Arabidopsis Sequence Match ( AT5G25980.1 )
(BLAST)

001: MQHNTYIYIL TMKLLGFALA ILLVVATCKP EEEITCEENV PFTCSQTDRF NKQDFESDFI FGVASSAYQI EGGRGRGLNV WDGFTHRYPE KGGADLGNGD
101: TTCDSYRTWQ KDLDVMEELG VKGYRFSFAW SRILPKGKRS RGINEDGINY YSGLIDGLIA RNITPFVTLF HWDLPQSLQD EYEGFLDRTI IDDFKDYADL
201: CFERFGDRVK HWITINQLFT VPTRGYALGT DAPGRCSQWV DKRCYGGDSS TEPYIVAHNQ LLAHATVVDL YRTRYKYQGG KIGPVMITRW FLPYDDTLES
301: KQATWRAKEF FLGWFMEPLT KGKYPYIMRK LVGNRLPKFN STEARLLKGS YDFLGLNYYV TQYAHALDPS PPEKLTAMTD SLANLTSLDA NGQPPGPPFS
401: KGSYYHPRGM LNVMEHFKTK YGDPLIYVTE NGFSTSGGPI PFTEAFHDYN RIDYLCSHLC FLRKAIK

Arabidopsis Description

TGG2Myrosinase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9C5C2]
SUBAcon: [endoplasmic reticulum,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.