CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

extracellular 5
endoplasmic reticulum 6
vacuole 7
plasma membrane 5
golgi 5

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	vacuole
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	vacuole
YLoc:	endoplasmic reticulum

cytosol: 16847873

msms PMID: 16847873 doi

V Katavic, GK Agrawal, M Hajduch, SL Harris, JJ Thelen

University of Missouri-Columbia, Department of Biochemistry, Columbia 65211, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
CDY32970
CDY43592

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G26000.1	CDY32970	AT5G42980.1	15352244
AT5G26000.1	CDY43592	AT5G42980.1	15352244

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Bra039705.1-P	Field mustard	vacuole	99.45	99.82
CDY09042	Canola	vacuole	95.79	99.05
CDY39513	Canola	endoplasmic reticulum, vacuole	80.04	83.08
CDY46331	Canola	endoplasmic reticulum, vacuole	81.87	82.47
CDY09054	Canola	vacuole	71.98	79.88
CDY22731	Canola	cytosol	24.91	77.27
CDY72290	Canola	cytosol, mitochondrion, vacuole	24.73	72.58
CDY26308	Canola	cytosol	72.16	72.29
CDY67046	Canola	extracellular	71.79	71.79
CDY70871	Canola	mitochondrion, vacuole	29.67	71.05
CDY05334	Canola	cytosol	67.77	70.21
CDY07361	Canola	endoplasmic reticulum	68.5	69.52
CDY52022	Canola	cytosol	67.58	69.49
CDY37226	Canola	extracellular	69.96	69.08
CDY71022	Canola	cytosol	67.22	68.6
AT5G26000.1	Thale cress	endoplasmic reticulum, vacuole	67.58	68.21
CDY04261	Canola	vacuole	46.89	67.19
CDY29614	Canola	cytosol, peroxisome, vacuole	11.17	65.59
CDY24995	Canola	cytosol, plastid, vacuole	40.48	65.58
CDY24999	Canola	cytosol, mitochondrion	24.36	65.52
CDY65716	Canola	cytosol	16.3	65.44
CDY24996	Canola	cytosol	21.06	65.34
CDY68654	Canola	cytosol	21.25	64.44
CDY71771	Canola	cytosol, vacuole	39.56	59.18
CDY52412	Canola	cytosol	9.34	57.3
CDY22730	Canola	mitochondrion	43.22	52.21
CDX90772	Canola	mitochondrion	47.8	44.09
CDY46560	Canola	cytosol, vacuole	7.33	40.0
CDY29604	Canola	mitochondrion, plastid, vacuole	11.9	38.69
CDX87822	Canola	plasma membrane	8.06	34.92
CDY67044	Canola	cytosol	0.73	23.53

Protein Annotations

Gene3D:3.20.20.80	MapMan:9.5.2.1	GO:A0A078GBJ8	UniProt:A0A078GBJ8	EnsemblPlants:CDY22724	ProteinID:CDY22724
ProteinID:CDY22724.1	GO:GO:0003674	GO:GO:0003824	GO:GO:0004553	GO:GO:0005975	GO:GO:0008150
GO:GO:0008152	GO:GO:0016787	GO:GO:0016798	EnsemblPlantsGene:GSBRNA2T00021024001	InterPro:Glyco_hydro_1	InterPro:Glyco_hydro_1_AS
InterPro:Glycoside_hydrolase_SF	PFAM:PF00232	PRINTS:PR00131	ScanProsite:PS00572	PANTHER:PTHR10353	PANTHER:PTHR10353:SF44
SUPFAM:SSF51445	SignalP:SignalP-noTM	UniParc:UPI0004EDFFB7	SEG:seg	:	:

Description

BnaA08g05540DBnaA08g05540D protein [Source:UniProtKB/TrEMBL;Acc:A0A078GBJ8]

Coordinates

chrLK032135:-:48886..51756

Molecular Weight (calculated)

62453.6 Da

IEP (calculated)

6.346

GRAVY (calculated)

-0.429

Length

546 amino acids

Sequence
(BLAST)

001: MKHLGLILAF LLALATCKAD EEITCEENLP FKCSQPDRLN SSSFEKDFIF GVASSAYQAC CLGRGLNVWD GFTHRYPNKS GPDHGNGDTT CDSFSYWQKD
101: IDVLDELNAT GYRFSIAWSR IIPRGKRSRG VNKDGINYYH GLIDGLIDKG ITPFVTLFHW DLPQVLQDEY EGFLDPQIIH DFKDYANLCF QEFGHKVKNW
201: LTINQLYTVP TRGYGAGSDA PGRCSPMVDP TCYAGNSSTE PYIVAHNQLL AHATVVDLYR KNYSHQGGKI GPVMITRWFL PYNDTDPDSI AATERMKEFF
301: LGWYMFMGPL TNGTYPQIMI DTVGDRLPSF SPEESNLVKG SYDFLGLNYY VTQYAQPSPN PVHWANHTAM MDAGAKLTYI NASNHYIGPL FEANSDETEN
401: SYYYPKGIYY VMDYFKTKYY NPLIYVTENG ISTPGNETRD ESMFDYKRIE YLCSHLCFLS KVIKEKHVNV KGYFAWSLGD NYEFDKGFTV RFGLSYIDWN
501: NVTDRDLKLS GKWYQKFISP AIKNPLKKDF LRSSLTFEKN KKFADA

Best Arabidopsis Sequence Match ( AT5G25980.2 )
(BLAST)

001: MQHNTYIYIL TMKLLGFALA ILLVVATCKP EEEITCEENV PFTCSQTDRF NKQDFESDFI FGVASSAYQI EGGRGRGLNV WDGFTHRYPE KGGADLGNGD
101: TTCDSYRTWQ KDLDVMEELG VKGYRFSFAW SRILPKGKRS RGINEDGINY YSGLIDGLIA RNITPFVTLF HWDLPQSLQD EYEGFLDRTI IDDFKDYADL
201: CFERFGDRVK HWITINQLFT VPTRGYALGT DAPGRCSQWV DKRCYGGDSS TEPYIVAHNQ LLAHATVVDL YRTRYKYQGG KIGPVMITRW FLPYDDTLES
301: KQATWRAKEF FLGWFMEPLT KGKYPYIMRK LVGNRLPKFN STEARLLKGS YDFLGLNYYV TQYAHALDPS PPEKLTAMTD SLANLTSLDA NGQPPGPPFS
401: KGSYYHPRGM LNVMEHFKTK YGDPLIYVTE NGFSTSGGPI PFTEAFHDYN RIDYLCSHLC FLRKAIKEKR VNVKGYFVWS LGDNYEFCNG YTVRFGLSYV
501: DFNNVTADRD LKASGLWYQS FLRDTTKNQD ILRSSLPFKN GDRKSLT

Arabidopsis Description

TGG2Myrosinase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9C5C2]
SUBAcon: [endoplasmic reticulum,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.