CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 2
cytosol 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	nucleus

nucleus: 21132161
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89867
KRH14678
KRH73469

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G08350.1	KRG89867	AT2G27040.1	19343051
AT4G08350.1	KRH14678	AT2G27040.1	19343051
AT4G08350.1	KRH73469	AT2G27040.1	19343051

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH53382	Soybean	nucleus	95.24	96.25
VIT_18s0001g15400.t01	Wine grape	cytosol	78.38	79.59
Zm00001d047048_P001	Maize	cytosol, mitochondrion, peroxisome	10.86	78.08
AT4G08350.1	Thale cress	nucleus	72.57	73.2
CDY34005	Canola	cytosol	72.1	72.1
CDX83142	Canola	cytosol	71.43	72.05
Bra000641.1-P	Field mustard	cytosol	71.43	72.05
Solyc04g064700.2.1	Tomato	nucleus	71.33	72.02
Os06t0208300-01	Rice	cytosol	19.71	71.13
GSMUA_Achr3P08080_001	Banana	cytosol	67.81	70.78
PGSC0003DMT400063938	Potato	nucleus	56.38	69.89
CDX75158	Canola	cytosol	43.81	69.17
EER88041	Sorghum	nucleus	67.71	67.71
CDY36201	Canola	cytosol	13.81	67.13
TraesCS7B01G073100.1	Wheat	cytosol	66.95	66.89
TraesCS7A01G168300.1	Wheat	nucleus	66.57	66.57
HORVU7Hr1G035280.12	Barley	cytosol	66.67	66.41
TraesCS7D01G169200.1	Wheat	cytosol	66.48	66.35
Zm00001d037142_P001	Maize	cytosol	65.71	66.35
Zm00001d044436_P001	Maize	extracellular, mitochondrion	7.62	66.12
Bra037857.1-P	Field mustard	cytosol	15.33	64.14
OQU85804	Sorghum	cytosol	60.95	63.18
AT2G34210.1	Thale cress	nucleus	58.67	62.29
Zm00001d044971_P005	Maize	cytosol, nucleus, plastid	63.9	62.24
CDY61565	Canola	nucleus	57.52	61.63
TraesCS6A01G325100.2	Wheat	nucleus	60.76	61.46
TraesCS6D01G304800.1	Wheat	nucleus	60.86	61.44
TraesCS6B01G355400.1	Wheat	nucleus	60.38	61.14
Bra022968.1-P	Field mustard	nucleus	58.19	59.9
CDX84655	Canola	nucleus	57.9	59.55
Os02t0772000-00	Rice	nucleus	18.95	57.51
Bra037858.1-P	Field mustard	cytosol	4.48	56.63
KRH35991	Soybean	nucleus	23.52	17.01
KRG90770	Soybean	nucleus	23.81	16.72
KRG90810	Soybean	mitochondrion	11.33	13.28

Protein Annotations

EntrezGene:100784916	MapMan:15.3.6.3.2	Gene3D:2.30.30.30	Gene3D:3.30.70.940	EMBL:ACUP02002767	EnsemblPlantsGene:GLYMA_04G240900
GO:GO:0003674	GO:GO:0003676	GO:GO:0003677	GO:GO:0003723	GO:GO:0003729	GO:GO:0003735
GO:GO:0005198	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005654	GO:GO:0005737	GO:GO:0005840	GO:GO:0006139	GO:GO:0006351	GO:GO:0006355
GO:GO:0006357	GO:GO:0006368	GO:GO:0006397	GO:GO:0006412	GO:GO:0008150	GO:GO:0008152
GO:GO:0009058	GO:GO:0009987	GO:GO:0019538	GO:GO:0032044	GO:GO:0032784	UniProt:I1JYX6
InterPro:IPR014722	InterPro:IPR036735	InterPro:KOW	EnsemblPlants:KRH64551	ProteinID:KRH64551	ProteinID:KRH64551.1
InterPro:NGN_dom	InterPro:NGN_dom_sf	PFAM:PF00467	PFAM:PF03439	PFAM:PF11942	PIRSF:PIRSF036945
ScanProsite:PS01108	InterPro:Rib_L2_dom2	InterPro:Ribosomal_L24/26_CS	SMART:SM00738	SMART:SM00739	SUPFAM:SSF50104
InterPro:Spt5_N	InterPro:TF_Spt5	InterPro:TF_Spt5_NGN-domain	InterPro:Translation_prot_SH3-like_sf	UniParc:UPI000233A09D	SEG:seg

Description

hypothetical protein

Coordinates

chr4:-:50913003..50922594

Molecular Weight (calculated)

116203.0 Da

IEP (calculated)

4.960

GRAVY (calculated)

-0.653

Length

1050 amino acids

Sequence
(BLAST)

0001: MSRRGMDDDD EEEYEEQDEQ VADFDEEEDE QVADFVEEEE EGSHRGRGGG GGRKRGRSGF IDDDAEEVDE DEEEEDDDDE DFDGRGGGGG RRRQYKKVSA
0101: SNFFDEEAVV DSDEEEEEEE GEDDFIVEGG SDLPEEDDGR RMRSSRMLPH HQEDHEDLEA MARSIQERYG RRLTDYDEET TDVEQQALLP SVRDPKLWMV
0201: KCAIGRERET AVCLMQKYID KGSELQIRSA IALDHLKNYI YVEADKEAHV REACKGLRNI FGQKITLVPI REMTDVLSVE SKAIDLARDT WVRLKIGTYK
0301: GDLAKVVDVD NVRQRVTVKL IPRIDLQALA NKLEGREVVK KKAFVPPPRF MNVDEARELH IRVEHRRDAY GERFDAIGGM MFKDGFLYKT VSIKSISAQN
0401: IKPTFDELEK FRKPGESGDG DVASLSTLFA NRKKGHFMKG DAVIVVKGDL KNLKGKVEKV DEDNVHIRPE MEDLPKTIAV NEKELCKYFE PGNHVKVVSG
0501: AQEGATGMVV KVEQHVLILI SDTTKEHIRV FADDVVESSE VTTGVTRIGD YELRDLVLLD NNSFGVIIRV ESEAFQVLKG IPDRPEVVLV KLREIKCKID
0601: KKISVQDRFK NTVSSKDVVR IIDGPCKGKQ GPVEHIYRGI LFIFDRHHLE HAGFICAKAQ SCVVVGGSRS SGDRNGDAYS RFASLRSPSR IPPSPRRFSR
0701: GGPMDSGGRH RGGRGHDSLA GTTVKVRQGP YKGYRGRVID VKGTTVRVEL ESQMKVVTVD RNHISDNVAV TPYRDTSRYG MGSETPMHPS RTPLHPYMTP
0801: MRDPGATPIH DGMRTPMRDP AWNPYTPMSP PRDNWEDGNP GSWAASPQYQ PGSPPSRPYE APTPGAGWAS TPGGNYSEAG TPRDSSAYAN APSPYLPSTP
0901: GGQPMTPSSA SYLPGTPGGQ PMTPGTGGMD MMSPVLGGEN EGPWFIPDIL VNVHRAGEES VGVIREVLPD GSYRVALGSS GNGEAITALP NEMEAVVPRK
1001: SDKIKIMGGA LRGATGKLIG VDGTDGIVKV DDTLDVKILD LVILAKLAQP

Best Arabidopsis Sequence Match ( AT4G08350.1 )
(BLAST)

0001: MPRSRDEDDE LDGDYEALDL EEEEEEDEEE EEERGRGGGG SRRKRGRSNF IDDYAEEDSQ EEDDDDEDYG SSRGGKGAAS KRKKPSASIF LDREAHQVDD
0101: EDEEEEDEAE DDFIVDNGTD LPDERGDRRY ERRFLPRDEN DEDVEDLERR IQERFSSRHH EEYDEEATEV EQQALLPSVR DPKLWMVKCA IGREREVAVC
0201: LMQKFIDRGA DLQIRSVVAL DHLKNFIYVE ADKEAHVKEA IKGMRNIYAN QKILLVPIRE MTDVLSVESK AIDLSRDTWV RMKIGTYKGD LAKVVDVDNV
0301: RQRVTVKLIP RIDLQALASK LDGREVSKKK AFVPPPRFMN IDEARELHIR VERRRDHMTG DYFENIGGML FKDGFHYKQV SLKSITVQNV TPTFDELEKF
0401: NKPSENGEGD FGGLSTLFAN RKKGHFMKGD AVIVIKGDLK NLKGWVEKVD EENVLIRSEV KGLPDPLAVN ERELCKYFEP GNHVKVVSGT HEGATGMVVK
0501: VDQHVLIILS DTTKEHVRVF ADHVVESSEV TTGVTKIGDY ELHDLVLLDN LSFGVIIRLE NEAFQVLKGV PDRPEVALVK LREIKCKLEK KINVQDRYKN
0601: VIAVKDDVRV IEGPSKGKQG PVKHIYKGVL FIYDRHHLEH AGFICAKCTS CIVVGGSRSG ANRNGGDSLS RYGNFKAPAP VPSSPGRFQR GRGGGYNNSG
0701: GRHGGGRGRG DDSLLGTTVK IRLGPFKGYR GPVVEVKGNS VRVELEMKIV TVDRGAISDN VATTPFRDTS RYSMGSETPM HPSRTPLHPY MTPMRDSGAT
0801: PIHDGMRTPM RDRAWNPYTP MSPPRDNWED GNPGSWGTSP QYQPGSPPSR AYEAPTPGSG WASTPGGSYS DAGTPRDHGS AYANAPSPYL PSTPGQPMTP
0901: SSASYLPGTP GGQPMTPGTG LDVMSPVIGG DAEAWFMPDI LVDIHKAGED TDVGVIRDVS DGTCKVSLGS SGEGDTIMAL PSELEIIPPR KSDRVKIVGG
1001: QYRGSTGKLI GIDGSDGIVK IDDNLDVKIL DLALLAKFVQ P

Arabidopsis Description

GTA02Putative transcription elongation factor SPT5 homolog 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9STN3]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.