CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 5
plastid 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG90770
KRH13386
KRH20723
KRH35991

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G27040.1	KRH13386	AT2G40030.1	19410546
AT2G27040.1	KRH20723	AT2G40030.1	19410546
AT2G27040.1	KRG90770	AT5G04290.1	19410546
AT2G27040.1	KRH35991	AT5G04290.1	19410546

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH14678	Soybean	nucleus	97.24	97.24
VIT_06s0009g01200.t01	Wine grape	nucleus	81.24	80.61
KRG89867	Soybean	nucleus	83.22	79.62
PGSC0003DMT400058471	Potato	nucleus	78.7	78.44
Solyc01g008960.2.1	Tomato	nucleus	78.37	78.11
PGSC0003DMT400069459	Potato	nucleus	78.26	77.66
Solyc06g073540.2.1	Tomato	nucleus	77.81	77.22
AT2G27040.1	Thale cress	nucleus	73.29	71.86
CDY56413	Canola	nucleus	73.07	71.72
CDY48192	Canola	nucleus	72.96	71.69
CDY04475	Canola	nucleus	72.96	71.61
Bra034318.1-P	Field mustard	nucleus	72.85	71.58
CDX77061	Canola	nucleus	72.74	71.48
Zm00001d040429_P012	Maize	nucleus	70.86	71.25
GSMUA_Achr9P05150_001	Banana	nucleus	71.85	71.15
Solyc06g073530.1.1	Tomato	nucleus	70.97	71.13
Zm00001d008249_P012	Maize	nucleus	70.53	71.0
OQU86696	Sorghum	nucleus	70.2	70.67
Os01t0275600-01	Rice	nucleus	70.31	70.46
Os04t0151800-02	Rice	nucleus	70.42	70.03
TraesCS3A01G188400.1	Wheat	nucleus	69.65	68.89
HORVU3Hr1G038830.1	Barley	nucleus	69.43	68.22
EES18816	Sorghum	nucleus	67.0	66.78
TraesCS3B01G217300.2	Wheat	golgi, nucleus, unclear	69.54	66.74
TraesCS1D01G453600.1	Wheat	nucleus	66.0	64.86
TraesCS1A01G445500.1	Wheat	golgi, unclear	66.0	64.65
TraesCS1B01G480100.1	Wheat	nucleus	66.11	64.62
Zm00001d039214_P025	Maize	nucleus	65.78	64.5
TraesCS7D01G458800.1	Wheat	nucleus	32.78	63.73
TraesCS7A01G533100.1	Wheat	cytosol	63.24	63.6
TraesCS7D01G520400.1	Wheat	nucleus	63.47	63.26
TraesCS7B01G450300.1	Wheat	nucleus	63.8	62.89
TraesCS3D01G191600.5	Wheat	nucleus	69.54	62.87
TraesCS7B01G450200.2	Wheat	nucleus	61.15	59.83
TraesCS7A01G521000.1	Wheat	nucleus	52.32	58.45
TraesCS7B01G437900.1	Wheat	cytosol	51.43	57.74
EES00570	Sorghum	nucleus	56.4	56.9
KRH20672	Soybean	nucleus	57.28	56.85
TraesCS7B01G437700.1	Wheat	nucleus	54.86	56.54
Os01t0275200-00	Rice	golgi, peroxisome, plastid	28.48	55.25
KRH56276	Soybean	cytosol	50.66	55.23
TraesCS7B01G373400.1	Wheat	nucleus	44.81	51.65
TraesCS7D01G458900.1	Wheat	mitochondrion	9.38	46.7
HORVU7Hr1G107770.1	Barley	nucleus	30.68	46.49
CDY62055	Canola	nucleus	28.92	45.49
KRG99173	Soybean	cytosol	7.51	43.59
KRH03770	Soybean	nucleus	34.0	34.11
KRH54842	Soybean	nucleus	34.11	33.99
KRH63036	Soybean	cytosol	34.0	33.88
KRH56656	Soybean	cytosol	33.66	33.59
KRH35431	Soybean	cytosol	34.22	31.83
KRH25139	Soybean	plastid	33.66	31.74
KRH31645	Soybean	nucleus	33.22	31.72
KRH69078	Soybean	cytosol	34.0	31.69
KRG91384	Soybean	cytosol	34.0	31.62
KRH30536	Soybean	plastid	31.02	31.57
KRH09472	Soybean	nucleus	35.76	30.77
KRH38925	Soybean	endoplasmic reticulum	35.76	30.62
KRH36727	Soybean	nucleus	14.13	28.26
KRG89432	Soybean	nucleus	27.81	26.09
KRH70802	Soybean	nucleus	29.47	25.9
KRH74941	Soybean	nucleus	29.14	25.66
KRH11732	Soybean	nucleus	26.71	23.34

Protein Annotations

EntrezGene:100792105	MapMan:12.5.1.13	Gene3D:2.170.260.10	Gene3D:3.30.420.10	Gene3D:3.40.50.2300	EMBL:ACUP02001278
InterPro:ArgoL1	InterPro:ArgoL2	InterPro:Argonaute_N	EnsemblPlantsGene:GLYMA_02G274900	GO:GO:0003674	GO:GO:0003676
GO:GO:0005488	GO:GO:0005515	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0031047
UniProt:I1JIR2	InterPro:IPR003100	InterPro:IPR003165	InterPro:IPR036397	EnsemblPlants:KRH73469	ProteinID:KRH73469
ProteinID:KRH73469.1	InterPro:PAZ_dom	InterPro:PAZ_dom_sf	PFAM:PF02170	PFAM:PF02171	PFAM:PF08699
PFAM:PF16486	PFAM:PF16488	PFscan:PS50821	PFscan:PS50822	PANTHER:PTHR22891	PANTHER:PTHR22891:SF42
InterPro:Piwi	InterPro:RNaseH-like_sf	InterPro:RNaseH_sf	SMART:SM00950	SMART:SM01163	SUPFAM:SSF101690
SUPFAM:SSF53098	UniParc:UPI0002337046	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr2:+:45798284..45806511

Molecular Weight (calculated)

101158.0 Da

IEP (calculated)

9.177

GRAVY (calculated)

-0.382

Length

906 amino acids

Sequence
(BLAST)

001: MASSGPNGTD NGDSLPPPPP VVPSDVVPVK AELEKKKALR LPIARRGLAS KGTKLQLLTN HYRVNVANTD GHFYQYSVAL FYDDGRPVEG KGVGRKLLDR
101: VHETYDSELN GKDFAYDGEK TLFTLGSLAR NKLEFTVVLE DIIASRNNGN CSPDGNGELN ESDKKRMRRP NSSKAFKVEL SYASKIPLQA IANALRGQES
201: ENYQEAIRVL DIILRQHAAK QGCLLVRQSF FHNDPKNFAD VGGGVLGCRG FHSSFRTTQS GLSLNIDVST TMIITPGPVV DFLISNQNVR DPFSLDWAKA
301: KRTLKNLRIK ASPSNQEFKI TGISEFPCKD QTFTLKRKGG DDVAEEEVTV YDYFVNIRKI DLRYSGDLPC INVGKPKRPT YIPLELCSLV SLQRYTKALS
401: TLQRASLVEK SRQKPQERMR VLTDALKSSN YGSEPMLRNC GISISPNFTE VEGRVLQAPR LKFGNGEDFN PRNGRWNFNN KKIVKPTKIE RWAVVNFSAR
501: CDTRGLVRDL IKCGGMKGIV IDQPFDVFEE NGQFRRAPPV VRVEKMFELV QSKLPGAPQF LLCLLPERKN SDLYGPWKKK NLAEFGIVTQ CIAPTRVNDQ
601: YLTNVLLKIN AKLGGLNSIL GVEHSPSIPI VSRAPTIIIG MDVSHGSPGQ TDIPSIAAVV SSREWPLISK YRASVRTQSP KMEMIDNLFK KVSDKEDEGI
701: MRELLLDFYT SSGNRKPDNI IIFRDGVSES QFNQVLNIEL DQIIEACKFL DEKWNPKFLV IVAQKNHHTK FFQPGAPDNV PPGTVIDNKI CHPRNYDFYM
801: CAHAGMIGTS RPTHYHVLLD EIGFSPDDLQ ELVHSLSYVY QRSTTAISVV APICYAHLAA TQMGQFMKFE DKSETSSSHG GSGMPAPPVP QLPRLQENVS
901: SSMFFC

Best Arabidopsis Sequence Match ( AT2G27040.2 )
(BLAST)

001: MDSTNGNGAD LESANGANGS GVTEALPPPP PVIPPNVEPV RVKTELAEKK GPVRVPMARK GFGTRGQKIP LLTNHFKVDV ANLQGHFFHY SVALFYDDGR
101: PVEQKGVGRK ILDKVHQTYH SDLDGKEFAY DGEKTLFTYG ALPSNKMDFS VVLEEVSATR ANGNGSPNGN ESPSDGDRKR LRRPNRSKNF RVEISYAAKI
201: PLQALANAMR GQESENSQEA IRVLDIILRQ HAARQGCLLV RQSFFHNDPT NCEPVGGNIL GCRGFHSSFR TTQGGMSLNM DVTTTMIIKP GPVVDFLIAN
301: QNARDPYSID WSKAKRTLKN LRVKVSPSGQ EFKITGLSDK PCREQTFELK KRNPNENGEF ETTEVTVADY FRDTRHIDLQ YSADLPCINV GKPKRPTYIP
401: LELCALVPLQ RYTKALTTFQ RSALVEKSRQ KPQERMTVLS KALKVSNYDA EPLLRSCGIS ISSNFTQVEG RVLPAPKLKM GCGSETFPRN GRWNFNNKEF
501: VEPTKIQRWV VVNFSARCNV RQVVDDLIKI GGSKGIEIAS PFQVFEEGNQ FRRAPPMIRV ENMFKDIQSK LPGVPQFILC VLPDKKNSDL YGPWKKKNLT
601: EFGIVTQCMA PTRQPNDQYL TNLLLKINAK LGGLNSMLSV ERTPAFTVIS KVPTIILGMD VSHGSPGQSD VPSIAAVVSS REWPLISKYR ASVRTQPSKA
701: EMIESLVKKN GTEDDGIIKE LLVDFYTSSN KRKPEHIIIF RDGVSESQFN QVLNIELDQI IEACKLLDAN WNPKFLLLVA QKNHHTKFFQ PTSPENVPPG
801: TIIDNKICHP KNNDFYLCAH AGMIGTTRPT HYHVLYDEIG FSADELQELV HSLSYVYQRS TSAISVVAPI CYAHLAAAQL GTFMKFEDQS ETSSSHGGIT
901: APGPISVAQL PRLKDNVANS MFFC

Arabidopsis Description

AGO4Protein argonaute 4 [Source:UniProtKB/Swiss-Prot;Acc:Q9ZVD5]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.