CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 5
plasma membrane 1
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG90770
KRH13386
KRH20723
KRH35991

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G27040.1	KRH13386	AT2G40030.1	19410546
AT2G27040.1	KRH20723	AT2G40030.1	19410546
AT2G27040.1	KRG90770	AT5G04290.1	19410546
AT2G27040.1	KRH35991	AT5G04290.1	19410546

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH14678	Soybean	nucleus	80.04	83.66
KRH73469	Soybean	nucleus	79.62	83.22
VIT_06s0009g01200.t01	Wine grape	nucleus	76.35	79.19
PGSC0003DMT400069459	Potato	nucleus	75.08	77.88
Solyc06g073540.2.1	Tomato	nucleus	74.87	77.66
Solyc01g008960.2.1	Tomato	nucleus	73.28	76.35
PGSC0003DMT400058471	Potato	nucleus	73.18	76.24
CDY48192	Canola	nucleus	69.48	71.37
Bra034318.1-P	Field mustard	nucleus	69.38	71.26
Solyc06g073530.1.1	Tomato	nucleus	67.9	71.13
CDY04475	Canola	nucleus	69.27	71.07
CDX77061	Canola	nucleus	69.17	71.04
CDY56413	Canola	nucleus	69.17	70.96
AT2G27040.1	Thale cress	nucleus	68.64	70.35
Zm00001d008249_P012	Maize	nucleus	66.63	70.11
GSMUA_Achr9P05150_001	Banana	nucleus	67.48	69.84
Zm00001d040429_P012	Maize	nucleus	66.42	69.81
Os01t0275600-01	Rice	nucleus	66.63	69.8
Os04t0151800-02	Rice	nucleus	66.63	69.26
OQU86696	Sorghum	nucleus	65.79	69.22
TraesCS3A01G188400.1	Wheat	nucleus	66.0	68.23
HORVU3Hr1G038830.1	Barley	nucleus	65.79	67.57
EES18816	Sorghum	nucleus	64.63	67.33
TraesCS3B01G217300.2	Wheat	golgi, nucleus, unclear	65.79	66.0
Zm00001d039214_P025	Maize	nucleus	63.99	65.58
TraesCS1D01G453600.1	Wheat	nucleus	63.57	65.29
TraesCS1A01G445500.1	Wheat	golgi, unclear	63.67	65.19
TraesCS1B01G480100.1	Wheat	nucleus	63.67	65.05
TraesCS7A01G533100.1	Wheat	cytosol	61.77	64.93
TraesCS7D01G520400.1	Wheat	nucleus	61.14	63.7
TraesCS7B01G450300.1	Wheat	nucleus	60.72	62.57
TraesCS3D01G191600.5	Wheat	nucleus	65.79	62.18
TraesCS7B01G450200.2	Wheat	nucleus	59.35	60.69
TraesCS7D01G458800.1	Wheat	nucleus	29.67	60.3
TraesCS7A01G521000.1	Wheat	nucleus	48.79	56.97
TraesCS7B01G437900.1	Wheat	cytosol	48.47	56.88
EES00570	Sorghum	nucleus	53.43	56.35
KRH20672	Soybean	nucleus	54.07	56.08
TraesCS7B01G437700.1	Wheat	nucleus	51.95	55.97
KRH56276	Soybean	cytosol	47.94	54.63
Os01t0275200-00	Rice	golgi, peroxisome, plastid	26.29	53.32
TraesCS7B01G373400.1	Wheat	nucleus	43.08	51.91
TraesCS7D01G458900.1	Wheat	mitochondrion	9.5	49.45
HORVU7Hr1G107770.1	Barley	nucleus	29.46	46.66
CDY62055	Canola	nucleus	28.19	46.35
KRG99173	Soybean	cytosol	6.97	42.31
KRH03770	Soybean	nucleus	34.0	35.66
KRH63036	Soybean	cytosol	34.11	35.53
KRH54842	Soybean	nucleus	34.0	35.42
KRH56656	Soybean	cytosol	33.9	35.35
KRH35431	Soybean	cytosol	34.64	33.68
KRG91384	Soybean	cytosol	34.42	33.47
KRH69078	Soybean	cytosol	34.32	33.44
KRH31645	Soybean	nucleus	33.26	33.19
KRH25139	Soybean	plastid	33.37	32.88
KRH30536	Soybean	plastid	30.2	32.13
KRH09472	Soybean	nucleus	35.27	31.72
KRH38925	Soybean	endoplasmic reticulum	35.27	31.57
KRH36727	Soybean	nucleus	14.36	30.02
KRG89432	Soybean	nucleus	27.35	26.81
KRH70802	Soybean	nucleus	28.19	25.9
KRH74941	Soybean	nucleus	27.98	25.75
KRH11732	Soybean	nucleus	27.03	24.69

Protein Annotations

EntrezGene:100820376	MapMan:12.5.1.13	Gene3D:2.170.260.10	Gene3D:3.30.420.10	Gene3D:3.40.50.2300	EMBL:ACUP02012299
InterPro:ArgoL1	InterPro:ArgoL2	InterPro:Argonaute_Mid_dom	InterPro:Argonaute_N	EnsemblPlantsGene:GLYMA_20G052500	GO:GO:0003674
GO:GO:0003676	GO:GO:0005488	GO:GO:0005515	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987
GO:GO:0031047	InterPro:IPR003100	InterPro:IPR003165	InterPro:IPR036397	UniProt:K7N1Q0	EnsemblPlants:KRG89867
ProteinID:KRG89867	ProteinID:KRG89867.1	InterPro:PAZ_dom	InterPro:PAZ_dom_sf	PFAM:PF02170	PFAM:PF02171
PFAM:PF08699	PFAM:PF16486	PFAM:PF16487	PFAM:PF16488	PFscan:PS50821	PFscan:PS50822
InterPro:Piwi	InterPro:RNaseH-like_sf	InterPro:RNaseH_sf	SMART:SM00950	SMART:SM01163	SUPFAM:SSF101690
SUPFAM:SSF53098	UniParc:UPI00029579E0	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr20:+:11866297..11875087

Molecular Weight (calculated)

106134.0 Da

IEP (calculated)

8.892

GRAVY (calculated)

-0.407

Length

947 amino acids

Sequence
(BLAST)

001: MNQRSPTHGT EPQRIIALLT HKRERETMTK EAMDSFEPDG NGKESLPPPP PVVPSDIVPL KAEEVLCTPT EHNKKKASRL PIARSGLGSK GNKIQLLTNH
101: FKVNVAKNDG HFFHYSVAFT YEDGRPVEGK GVGRKIIDRV QETYHSDLNG KDFAYDGEKS LFTVGSLPQN KLEFEVVLED VTSNRNNGNC SPDGLGDNES
201: DRKRMRRPYR SKSFKVEISF AAKIPMQAIA SALRGQETEN FQEAIRVLDI ILRQHAAKQG CLLVRQSFFH NNPNNFADVG GGVLGCRGFH SSFRTTQSGL
301: SLNIDVSTTM IISPGPVVDF LISNQNVRDP FQLDWAKAKR TLKNLRIKTS PSNQEFKISG LSELPCREQT FTLKGKGGGD GEDGNEEITV YDYFVKVRKI
401: DLRYSADLPC INVGKPKRPT FFPIEVCELV SLQRYTKALS TLQRASLVEK SRQKPQERMK ILSDALRTSN YGAEPMLRNC GISISTGFTE VEGRVLPAPR
501: LKFGNGEDLN PRNGRWNVSR VKFVEPSKIE RWAVANFSAR CDVRGLVRDL IRIGDMKGIT IEQPFDVFDE NPQFRRAPPM VRVEKMFEHI QSKLPGAPQF
601: LLCLLPDRKN CDIYGPWKKK NLADFGIINQ CMCPLRVNDQ YLTNVMLKIN AKLGGLNSLL GVEHSPSLPV VSKAPTLILG MDVSHGSPGQ TDIPSIAAVV
701: SSRHWPLISK YRACVRTQSA KMEMIDNLFK LVSEKEDEGI IRELLLDFYT TSGRRKPENI IIFRDGVSES QFNQVLNIEL DRIIEACKFL DENWEPKFVV
801: IVAQKNHHTR FFQPGSPDNV PPGTVIDNKI CHPRNYDFYL CAHAGMIGTS RPTHYHVLLD QVGFSPDQLQ ELVHSLSYVY QRSTTAISVV APICYAHLAA
901: TQLGQFMKFE DKSETSSSHG GLSGAGAVPV PQLPPLQENV RNTMFFC

Best Arabidopsis Sequence Match ( AT2G27040.2 )
(BLAST)

001: MDSTNGNGAD LESANGANGS GVTEALPPPP PVIPPNVEPV RVKTELAEKK GPVRVPMARK GFGTRGQKIP LLTNHFKVDV ANLQGHFFHY SVALFYDDGR
101: PVEQKGVGRK ILDKVHQTYH SDLDGKEFAY DGEKTLFTYG ALPSNKMDFS VVLEEVSATR ANGNGSPNGN ESPSDGDRKR LRRPNRSKNF RVEISYAAKI
201: PLQALANAMR GQESENSQEA IRVLDIILRQ HAARQGCLLV RQSFFHNDPT NCEPVGGNIL GCRGFHSSFR TTQGGMSLNM DVTTTMIIKP GPVVDFLIAN
301: QNARDPYSID WSKAKRTLKN LRVKVSPSGQ EFKITGLSDK PCREQTFELK KRNPNENGEF ETTEVTVADY FRDTRHIDLQ YSADLPCINV GKPKRPTYIP
401: LELCALVPLQ RYTKALTTFQ RSALVEKSRQ KPQERMTVLS KALKVSNYDA EPLLRSCGIS ISSNFTQVEG RVLPAPKLKM GCGSETFPRN GRWNFNNKEF
501: VEPTKIQRWV VVNFSARCNV RQVVDDLIKI GGSKGIEIAS PFQVFEEGNQ FRRAPPMIRV ENMFKDIQSK LPGVPQFILC VLPDKKNSDL YGPWKKKNLT
601: EFGIVTQCMA PTRQPNDQYL TNLLLKINAK LGGLNSMLSV ERTPAFTVIS KVPTIILGMD VSHGSPGQSD VPSIAAVVSS REWPLISKYR ASVRTQPSKA
701: EMIESLVKKN GTEDDGIIKE LLVDFYTSSN KRKPEHIIIF RDGVSESQFN QVLNIELDQI IEACKLLDAN WNPKFLLLVA QKNHHTKFFQ PTSPENVPPG
801: TIIDNKICHP KNNDFYLCAH AGMIGTTRPT HYHVLYDEIG FSADELQELV HSLSYVYQRS TSAISVVAPI CYAHLAAAQL GTFMKFEDQS ETSSSHGGIT
901: APGPISVAQL PRLKDNVANS MFFC

Arabidopsis Description

AGO4Protein argonaute 4 [Source:UniProtKB/Swiss-Prot;Acc:Q9ZVD5]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.