CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plastid, nucleus

Predictor Summary:

plastid 11
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG98542
KRH00049
KRH05125
KRH14245
KRH46343
KRH50190
KRH71273
KRH73892

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G59760.1	KRG98542	AT3G13110.1	11168407
AT3G59760.1	KRH00049	AT3G13110.1	11168407
AT3G59760.1	KRH05125	AT3G13110.1	11168407
AT3G59760.1	KRH14245	AT3G13110.1	11168407
AT3G59760.1	KRH46343	AT3G13110.1	11168407
AT3G59760.1	KRH73892	AT3G13110.1	11168407
AT3G59760.1	KRG98542	AT3G13110.1	12063244
AT3G59760.1	KRH00049	AT3G13110.1	12063244
AT3G59760.1	KRH05125	AT3G13110.1	12063244
AT3G59760.1	KRH14245	AT3G13110.1	12063244
AT3G59760.1	KRH46343	AT3G13110.1	12063244
AT3G59760.1	KRH73892	AT3G13110.1	12063244
AT3G59760.1	KRH50190	AT3G59760.1	11168407
AT3G59760.1	KRH71273	AT3G59760.1	11168407

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH50190	Soybean	nucleus, plastid	94.92	96.14
VIT_05s0094g00780.t01	Wine grape	plastid	80.2	82.72
GSMUA_Achr5P17120_001	Banana	plastid	74.62	76.56
Solyc08g014340.2.1	Tomato	plastid	74.11	75.65
PGSC0003DMT400059624	Potato	cytosol, plastid	74.11	75.65
GSMUA_Achr9P02430_001	Banana	plastid	72.08	74.74
Os01t0978100-01	Rice	plastid	74.62	74.62
CDY23400	Canola	plastid	73.1	74.04
CDY30438	Canola	plastid	73.1	74.04
KRG96937	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	60.91	73.85
Bra037682.1-P	Field mustard	plastid	72.84	73.78
KRH27640	Soybean	cytosol	60.66	73.54
KRG91325	Soybean	cytosol, mitochondrion, nucleus	60.66	73.54
AT2G43750.1	Thale cress	plastid	73.1	73.47
KRH68699	Soybean	cytosol	60.41	73.23
CDY17914	Canola	plastid	72.84	73.21
Zm00001d040235_P005	Maize	plastid	73.35	72.98
TraesCS3B01G102500.1	Wheat	golgi, plastid	72.33	72.7
TraesCS3A01G087100.1	Wheat	plastid	71.57	72.49
CDX79804	Canola	plastid	72.84	72.47
TraesCS3D01G087300.1	Wheat	plastid	72.08	72.45
EES02674	Sorghum	plastid	73.86	72.39
Bra004781.1-P	Field mustard	plastid	72.59	72.22
Zm00001d008379_P001	Maize	plastid	72.33	71.61
KRH13764	Soybean	cytosol	57.36	70.41
KRH34052	Soybean	cytosol	56.35	68.52
KRG92757	Soybean	cytosol	55.84	68.11
KRH34037	Soybean	cytosol	55.08	67.18
KRH35519	Soybean	cytosol	51.78	66.89
CDY45790	Canola	plastid	72.08	66.51
Bra014529.1-P	Field mustard	plastid	71.07	66.35
KRH77854	Soybean	cytosol	12.44	66.22
AT3G59760.1	Thale cress	plastid	72.59	66.05
CDX89032	Canola	plastid	71.32	65.5
KRH74905	Soybean	cytosol	13.2	65.0
KRH43690	Soybean	cytosol	38.32	64.81
KRH64961	Soybean	nucleus, plastid	54.82	57.75
KRG94935	Soybean	nucleus	54.31	57.68
KRG92761	Soybean	cytosol	45.69	54.38
KRH40428	Soybean	mitochondrion	50.76	53.62
KRH00797	Soybean	mitochondrion	50.51	53.49
KRH14231	Soybean	mitochondrion	26.14	24.24
KRH73904	Soybean	cytosol	21.32	19.91

Protein Annotations

KEGG:00270+2.5.1.47	KEGG:00920+2.5.1.47	EntrezGene:100815330	Gene3D:3.40.50.1100	MapMan:4.1.4.2.2	UniProt:A0A0R4J2P9
EMBL:ACUP02000882	InterPro:CysK	InterPro:Cys_synth	EnsemblPlantsGene:GLYMA_02G138400	GO:GO:0003674	GO:GO:0003824
GO:GO:0004124	GO:GO:0006535	GO:GO:0008150	GO:GO:0008152	GO:GO:0008652	GO:GO:0009058
GO:GO:0009987	GO:GO:0016740	GO:GO:0019344	EnsemblPlants:KRH71273	ProteinID:KRH71273	ProteinID:KRH71273.1
InterPro:P-phosphate_BS	PFAM:PF00291	InterPro:PLP-dep	ScanProsite:PS00901	PANTHER:PTHR10314	PANTHER:PTHR10314:SF160
MetaCyc:PWY-6936	SUPFAM:SSF53686	TIGRFAMs:TIGR01136	TIGRFAMs:TIGR01139	InterPro:Trypto_synt_PLP_dependent	UniParc:UPI0001B63990
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr2:-:14334884..14343397

Molecular Weight (calculated)

41650.2 Da

IEP (calculated)

7.373

GRAVY (calculated)

-0.047

Length

394 amino acids

Sequence
(BLAST)

001: MASSASLINS LTCSSPAPTQ HCFAFTRTTA TATATSLRQF NSHRPPPLTT RISSSPSTVV CKAVSVKPQT ETEGLNIADD VTQLIGKTPM VYLNNIVKGS
101: VANIAAKLEI MEPCCSVKDR IGFSMINDAE QRGAITPGKS ILVEPTSGNT GIGLAFIAAS RGYKLILTMP ASMSLERRVL LKAFGAELVL TDAAKGMNGA
201: VQKAEEILKS TPNSYMLQQF DNPSNPKVHY ETTGPEIWED TRGKIDILVA GIGTGGTVSG AGQFLKQQNP KIQVIGVEPL ESNILTGGKP GPHKIQGIGA
301: GFVPRNLDQD VLDEVIAISS DEAVETAKQL ALQEGLLVGI SSGAAAAAAL KVGKRPENAG KLIGVVFPSF GERYLSTILF QSIREECEKM QPEP

Best Arabidopsis Sequence Match ( AT2G43750.2 )
(BLAST)

001: MAATSSSAFL LNPLTSRHRP FKYSPELSSL SLSSRKAAAF DVSSAAFTLK RQSRSDVVCK AVSIKPEAGV EGLNIADNAA QLIGKTPMVY LNNVVKGCVA
101: SVAAKLEIME PCCSVKDRIG YSMITDAEEK GLITPGKSVL VESTSGNTGI GLAFIAASKG YKLILTMPAS MSLERRVLLR AFGAELVLTE PAKGMTGAIQ
201: KAEEILKKTP NSYMLQQFDN PANPKIHYET TGPEIWEDTR GKIDILVAGI GTGGTITGVG RFIKERKPEL KVIGVEPTES AILSGGKPGP HKIQGIGAGF
301: VPKNLDLAIV DEYIAISSEE AIETSKQLAL QEGLLVGISS GAAAAAAIQV AKRPENAGKL IAVVFPSFGE RYLSTQLFQS IREECEQMQP EL

Arabidopsis Description

OASBCysteine synthase [Source:UniProtKB/TrEMBL;Acc:Q0WW95]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.