CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 4
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion
BaCelLo:	cytosol
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH26049
KRH55364

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G28020.1	KRH26049	AT5G54390.1	21798944
AT5G28020.1	KRH55364	AT5G54390.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH34037	Soybean	cytosol	77.64	79.57
KRH43690	Soybean	cytosol	50.45	71.67
KRH13764	Soybean	cytosol	62.54	64.49
KRH34052	Soybean	cytosol	62.54	63.89
KRG92757	Soybean	cytosol	62.24	63.78
KRH77854	Soybean	cytosol	14.2	63.51
HORVU2Hr1G074630.2	Barley	cytosol	54.38	62.07
VIT_14s0030g01520.t01	Wine grape	cytosol	60.12	61.61
CDY50768	Canola	cytosol	58.01	59.81
CDY41911	Canola	cytosol	57.4	59.19
KRG96937	Soybean	cytosol, endoplasmic reticulum, mitochondrion, nucleus	58.01	59.08
KRH27640	Soybean	cytosol	58.01	59.08
AT3G04940.1	Thale cress	cytosol	57.7	58.95
CDY69239	Canola	cytosol	57.1	58.88
KRH68699	Soybean	cytosol	57.7	58.77
GSMUA_Achr3P30900_001	Banana	cytosol, unclear	57.1	58.15
GSMUA_Achr11P...	Banana	cytosol	56.8	58.02
CDX74160	Canola	cytosol	56.19	57.94
KXG24064	Sorghum	cytosol	56.8	57.85
KRG91325	Soybean	cytosol, mitochondrion, nucleus	56.8	57.85
GSMUA_Achr9P25760_001	Banana	cytosol	56.5	57.72
Zm00001d031136_P003	Maize	plasma membrane	56.5	57.54
Bra036115.1-P	Field mustard	cytosol	55.89	57.28
GSMUA_Achr4P21350_001	Banana	cytosol	56.19	57.23
VIT_14s0030g01550.t01	Wine grape	cytosol	58.91	57.02
AT5G28030.2	Thale cress	cytosol	55.59	56.97
AT5G28020.1	Thale cress	cytosol	54.98	56.35
CDY00007	Canola	cytosol	54.98	56.35
Solyc01g097950.2.1	Tomato	cytosol	54.68	56.04
HORVU3Hr1G040910.1	Barley	mitochondrion	5.74	55.88
CDY19703	Canola	cytosol	54.38	55.73
TraesCS5D01G027600.1	Wheat	cytosol, golgi	54.68	55.69
CDY52498	Canola	cytosol	54.08	55.42
CDY05586	Canola	cytosol	54.08	55.42
KRH35519	Soybean	cytosol	51.06	55.41
TraesCS2B01G316800.1	Wheat	cytosol	54.68	55.35
TraesCS2A01G300600.1	Wheat	cytosol	54.38	55.05
CDY41913	Canola	cytosol	50.45	54.93
CDX86187	Canola	cytosol	50.15	54.61
Solyc01g097930.2.1	Tomato	cytosol	52.87	54.18
CDX80680	Canola	cytosol	49.55	53.95
Solyc01g097920.2.1	Tomato	cytosol, plastid	56.8	52.81
CDY19702	Canola	mitochondrion	55.89	52.71
CDY00008	Canola	mitochondrion	55.89	52.71
KXG26295	Sorghum	cytosol	55.59	52.57
Solyc01g097940.1.1	Tomato	plasma membrane	31.12	52.55
Bra009985.1-P	Field mustard	cytosol	43.81	52.35
Os12t0625000-01	Rice	plasma membrane	54.68	52.16
CDY41915	Canola	cytosol	9.67	51.61
TraesCS2D01G298600.2	Wheat	cytosol	55.29	51.12
Bra036114.1-P	Field mustard	cytosol	52.57	50.88
Zm00001d003717_P001	Maize	cytosol	53.17	50.0
KRH74905	Soybean	cytosol	11.78	48.75
Bra001131.1-P	Field mustard	mitochondrion	56.5	46.75
KRH50190	Soybean	nucleus, plastid	54.38	46.27
KRH71273	Soybean	nucleus, plastid	54.38	45.69
HORVU5Hr1G015070.1	Barley	extracellular	9.37	45.59
KRG94935	Soybean	nucleus	50.45	45.01
VIT_14s0030g01490.t01	Wine grape	cytosol	59.52	44.87
KRH64961	Soybean	nucleus, plastid	50.15	44.38
KRH40428	Soybean	mitochondrion	47.73	42.36
KRH00797	Soybean	mitochondrion	47.43	42.2
TraesCS5A01G022200.1	Wheat	plastid	54.08	41.82
TraesCS5B01G018700.3	Wheat	plastid	54.68	41.23
HORVU5Hr1G005980.2	Barley	plastid	54.08	40.96
Bra020605.1-P	Field mustard	mitochondrion	52.57	40.09
HORVU5Hr1G082120.2	Barley	mitochondrion, plastid	22.36	39.57
Bra040417.1-P	Field mustard	cytosol	32.63	34.62
HORVU1Hr1G031220.3	Barley	plastid	33.53	27.27
KRH14231	Soybean	mitochondrion	29.31	22.82
KRH73904	Soybean	cytosol	24.17	18.96

Protein Annotations

KEGG:00270+2.5.1.47	KEGG:00920+2.5.1.47	Gene3D:3.40.50.1100	MapMan:50.2.5	UniProt:A0A0R0EF16	EMBL:ACUP02012766
InterPro:CysK	InterPro:Cys_synth	EnsemblPlantsGene:GLYMA_20G229000	GO:GO:0003674	GO:GO:0003824	GO:GO:0004124
GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006535
GO:GO:0008150	GO:GO:0008152	GO:GO:0008652	GO:GO:0009058	GO:GO:0009987	GO:GO:0016740
GO:GO:0019344	GO:GO:0030170	EnsemblPlants:KRG92761	ProteinID:KRG92761	ProteinID:KRG92761.1	InterPro:P-phosphate_BS
PFAM:PF00291	InterPro:PLP-dep	ScanProsite:PS00901	PANTHER:PTHR10314	PANTHER:PTHR10314:SF97	MetaCyc:PWY-6936
SUPFAM:SSF53686	TIGRFAMs:TIGR01136	TIGRFAMs:TIGR01139	InterPro:Trypto_synt_PLP_dependent	UniParc:UPI0006ED81F1	SEG:seg

Description

hypothetical protein

Coordinates

chr20:+:46306012..46310958

Molecular Weight (calculated)

35840.3 Da

IEP (calculated)

6.761

GRAVY (calculated)

-0.168

Length

331 amino acids

Sequence
(BLAST)

001: MDPHSHKLYD ADLLTACNQE RCHQTCRPSN MEPQCAIKKD VTELIGNTPM VYLNNIVEGC VARIAAKLES MQPCFSIKDR TAFSMIKDAE DKGLITPGKS
101: VLVEATSGNT GIGMAFVATL KGYKVIVAMP ASVSLERKIV LGAFGAEVYL TDPTKGTNAV LRKAEEIVNN TPGSYMLRQI DNPANPKIHY ETTGPEIWRD
201: SGGKVDALVA GVGTGGTITG AGRFLKERNP NVKLYGVEPV ESAVLSGGPP GDHQIQGIGN GLIPSVLDVN LLDEVIQISS EEAIETARLL ALKEDGNFIR
301: SRSCSCHKIG EKTRKCWKTN RCEFLIFDIM S

Best Arabidopsis Sequence Match ( AT3G04940.1 )
(BLAST)

001: MEEDRCSIKD DATQLIGNTP MVYLNNIVDG CVARIAAKLE MMEPCSSVKE RIAYGMIKDA EDKGLITPGK STLIEATSGN TGIGLAFIGA AKGYKVVLTM
101: PSSMSLERKI ILLALGAEVH LTDPSKGVQG IIDKAEEICS KNPDSIMLEQ FKNPSNPQTH YRTTGPEIWR DSAGEVDILV AGVGTGGTLS GSGRFLKEKN
201: KDFKVYGVEP TESAVISGGK PGTHLIQGIG AGLIPDNLDF NVLDEVIQVT SVEAIETAKL LALKEGLLVG ISSGAAAAAA IKVAKRPENA GKLIVVIFPS
301: GGERYLSTSL FESVRHEAEN LPIQ

Arabidopsis Description

CYSD1Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 [Source:UniProtKB/Swiss-Prot;Acc:Q9S6Z7]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.