CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

extracellular 3
endoplasmic reticulum 2
vacuole 2
plasma membrane 3
golgi 3
plastid 2
cytosol 1
mitochondrion 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, plastid, secretory
BaCelLo:	secretory
iPSORT:	plastid
MultiLoc:	extracellular
Plant-mPloc:	cytosol, golgi
Predotar:	mitochondrion
PProwler:	secretory
TargetP:	mitochondrion
WoLF PSORT:	plastid
YLoc:	plasma membrane

endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH05898
KRH12368
KRH17382
KRH37371
KRH49717
KRH51767
KRH61087

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G14720.1	KRH05898	AT4G16190.1	23738689
AT2G14720.1	KRH17382	AT4G16190.1	23738689
AT2G14720.1	KRH49717	AT4G16190.1	23738689
AT2G14720.1	KRH51767	AT4G16190.1	23738689
AT2G14720.1	KRH61087	AT4G16190.1	23738689
AT2G14720.1	KRH12368	AT5G20500.1	23738689
AT2G14720.1	KRH37371	AT5G20500.1	23738689

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH27575	Soybean	endoplasmic reticulum	92.69	94.9
KRG91109	Soybean	endoplasmic reticulum	84.14	85.74
KRH35666	Soybean	endoplasmic reticulum	83.98	85.58
CDY37219	Canola	extracellular, golgi, plasma membrane	80.09	82.53
CDX81459	Canola	plasma membrane	79.94	82.37
Zm00001d012390_P001	Maize	cytosol	11.51	82.22
AT2G14740.1	Thale cress	extracellular, plasma membrane, vacuole	80.25	82.17
Bra039817.1-P	Field mustard	extracellular, golgi, plasma membrane	79.63	82.05
AT2G14720.1	Thale cress	extracellular, plasma membrane, vacuole	79.78	81.69
CDY65441	Canola	plasma membrane	80.09	81.62
Bra013121.1-P	Field mustard	extracellular, plasma membrane, vacuole	79.94	81.46
CDX93144	Canola	extracellular, plasma membrane, vacuole	77.14	81.31
VIT_03s0091g00740.t01	Wine grape	plasma membrane	78.23	79.71
PGSC0003DMT400066159	Potato	endoplasmic reticulum, plasma membrane, vacuole	77.76	79.37
Solyc01g107130.2.1	Tomato	nucleus	77.45	79.05
Zm00001d004678_P001	Maize	cytosol, nucleus, plastid	12.29	78.22
GSMUA_Achr6P16900_001	Banana	cytosol, nucleus, plasma membrane	64.7	73.24
Os10t0346600-00	Rice	plasma membrane	70.3	71.63
Zm00001d014302_P003	Maize	plasma membrane	68.74	71.52
GSMUA_Achr10P...	Banana	cytosol	62.83	71.13
TraesCS1D01G131100.1	Wheat	golgi	69.83	70.93
TraesCS1B01G148200.1	Wheat	plasma membrane	69.21	70.41
TraesCS1A01G134600.2	Wheat	extracellular, plasma membrane, vacuole	69.52	70.06
Zm00001d014303_P002	Maize	plasma membrane	65.32	67.85
HORVU1Hr1G032780.9	Barley	extracellular	65.94	66.67
KRH00437	Soybean	endoplasmic reticulum, golgi, plasma membrane	63.92	66.4
KRH40723	Soybean	extracellular, golgi, plasma membrane	63.3	65.22
KRH37180	Soybean	plasma membrane	61.59	63.46
KRH12166	Soybean	plasma membrane	61.28	62.74
KRH48733	Soybean	extracellular, golgi, plasma membrane	60.65	62.7
KRH64888	Soybean	endoplasmic reticulum, golgi, plasma membrane	60.19	62.02

Protein Annotations

EntrezGene:100820115	Gene3D:2.10.25.10	MapMan:22.5.2.1	Gene3D:3.50.30.30	EMBL:ACUP02000686	InterPro:EGF-like_CS
InterPro:EGF-like_Ca-bd_dom	InterPro:EGF_Ca-bd_CS	EnsemblPlantsGene:GLYMA_01G242800	GO:GO:0003674	GO:GO:0005488	GO:GO:0005509
GO:GO:0005575	GO:GO:0016020	GO:GO:0016021	UniProt:I1JAY8	EnsemblPlants:KRH77935	ProteinID:KRH77935
ProteinID:KRH77935.1	InterPro:PA_domain	PFAM:PF02225	PFAM:PF12662	ScanProsite:PS01186	ScanProsite:PS01187
PANTHER:PTHR22765	PANTHER:PTHR22765:SF54	SMART:SM00179	SUPFAM:SSF52025	TMHMM:TMhelix	UniParc:UPI000295ACC8
InterPro:cEGF	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr1:+:56604160..56608116

Molecular Weight (calculated)

71214.8 Da

IEP (calculated)

5.463

GRAVY (calculated)

-0.328

Length

643 amino acids

Sequence
(BLAST)

001: MLYLVRIVTV IGVDSKKMKV WRWVVSLMLV GLLPVSTMGR FVVEKNSLRV TSPEKIRGTH DSAIGNFGIP QYGGSMAGNV IYPKDNKKGC KEFDEFGISF
101: KSTPGALPTI VLLDRGSCFF ALKVWNAQKA GASAVLVADD VEEPLITMDT PEEDGSSAKY VENITIPSAL VGKSFGLKLK DAFSGGDMVN LNLDWRESVP
201: HPDDRVEYEL WTNSNDECGV KCDMLMEFVK DFKGAAQILD KGGYTQFTPH YITWYCPHAF TLSKQCKSQC INHGRYCAPD PEQDFTTGYD GKDVVIENLR
301: QLCVFKVANE TEKPWVWWDY VTDFQIRCPM KEKKYNKECA NAVIKSLGLN TEKIEKCMGD PDADTDNPVL KEEQDAQIGK GSRGDVTILP TLVVNNRQYR
401: GKLEKGAVLK AICSGFEETT EPAVCLSSDV ETNECLTNNG GCWQDKAANI TACKDTFRGR VCECPLVDGV QFKGDGYTTC EASGLGRCKI NNGGCWHDAQ
501: NGHAFSACLD NGGVKCQCPT GFRGDGVKNC QDIDECKEKK ACQCPDCSCK NTWGSYDCSC SGDLLYMRDH DTCISKTGSQ GRSTWAAFWL ILLGVVMIAG
601: GAFLVYKYRI RQYMDSEIRA IMAQYMPLDS QAEVPNHVND QRA

Best Arabidopsis Sequence Match ( AT2G14740.1 )
(BLAST)

001: MKQLLCYLPW LLLLTLLVSP LNDARFVVEK NSLSVTSPES IKGTHDSAIG NFGIPQYGGS MAGTVVYPKE NQKSCKEFSD FSISFKSQPG ALPTFLLVDR
101: GDCFFALKVW NAQKAGASAV LVADNVDEPL ITMDTPEEDV SSAKYIENIT IPSALVTKGF GEKLKKAISG GDMVNLNLDW REAVPHPDDR VEYELWTNSN
201: DECGVKCDML MEFVKDFKGA AQILEKGGFT QFRPHYITWY CPHAFTLSRQ CKSQCINKGR YCAPDPEQDF SSGYDGKDVV VENLRQLCVY KVANETGKPW
301: VWWDYVTDFQ IRCPMKEKKY NKECADSVIK SLGIDSKKLD KCMGDPDADL DNPVLKEEQD AQVGKGSRGD VTILPTLVVN NRQYRGKLEK SAVLKALCSG
401: FEETTEPAIC LSTDVESNEC LDNNGGCWQD KSANITACKD TFRGRVCECP TVDGVQFKGD GYSHCEPSGP GRCTINNGGC WHEERDGHAF SACVDKDSVK
501: CECPPGFKGD GTKKCEDINE CKEKKACQCP ECSCKNTWGS YECSCSGDLL YIRDHDTCIS KTGAQVRSAW AAVWLIMLSL GLAAAGAYLV YKYRLRQYMD
601: SEIRAIMAQY MPLDSQPEIP NHVNDERA

Arabidopsis Description

VSR3VSR3 [Source:UniProtKB/TrEMBL;Acc:A0A178VT51]
SUBAcon: [plasma membrane,extracellular,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.