Factsheet for PGSC0003DMT400058018

Subcellular Localization

min:

: max

Winner_takes_all: vacuole

Predictor Summary:

extracellular 5
endoplasmic reticulum 5
vacuole 7
plasma membrane 6
golgi 5
nucleus 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	vacuole
Any Predictor:	nucleus, secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	vacuole
Plant-mPloc:	plasma membrane
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	nucleus
YLoc:	vacuole

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400022426
PGSC0003DMT400056956

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G17760.1	PGSC0003DMT400022426	AT4G26455.1	21798944
AT2G17760.1	PGSC0003DMT400056956	AT4G26455.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc03g033360.1.1	Tomato	plastid	66.54	93.57
PGSC0003DMT400009406	Potato	plasma membrane, vacuole	67.64	68.65
PGSC0003DMT400010765	Potato	cytosol	21.21	62.37
VIT_00s0510g00050.t01	Wine grape	plasma membrane, vacuole	28.88	51.63
KRH76603	Soybean	vacuole	47.53	51.18
KRH28845	Soybean	vacuole	46.44	50.0
Bra002086.1-P	Field mustard	extracellular, plasma membrane, vacuole	45.89	49.9
GSMUA_Achr4P05070_001	Banana	plasma membrane, vacuole	40.77	49.78
AT2G17760.1	Thale cress	golgi, plasma membrane, vacuole	46.62	49.71
CDY24371	Canola	extracellular, plasma membrane, vacuole	46.07	49.22
CDY07819	Canola	vacuole	45.89	49.12
Bra024490.1-P	Field mustard	vacuole	45.7	48.92
CDY29199	Canola	vacuole	45.7	48.92
KRH07991	Soybean	nucleus	44.97	47.67
GSMUA_Achr1P07400_001	Banana	cytosol, plasma membrane, plastid	13.89	47.5
GSMUA_Achr1P07410_001	Banana	cytosol	11.88	46.43
GSMUA_Achr1P07390_001	Banana	extracellular, vacuole	42.41	45.05
GSMUA_Achr2P03530_001	Banana	plasma membrane	42.41	44.53
GSMUA_AchrUn_...	Banana	plasma membrane, vacuole	36.38	44.32
CDY02252	Canola	golgi, plasma membrane, vacuole	25.23	44.23
PGSC0003DMT400009410	Potato	plasma membrane, vacuole	41.5	44.16
KXG25917	Sorghum	vacuole	41.68	44.1
Zm00001d022600_P001	Maize	endoplasmic reticulum, plasma membrane, vacuole	40.95	43.92
TraesCS2D01G065800.1	Wheat	vacuole	40.95	43.92
Zm00001d004132_P001	Maize	extracellular	41.32	43.63
EER99278	Sorghum	extracellular, plasma membrane, vacuole	41.5	43.4
TraesCS2B01G079700.1	Wheat	vacuole	40.77	43.39
TraesCS2A01G067500.1	Wheat	vacuole	40.4	43.08
GSMUA_Achr7P03880_001	Banana	extracellular	34.55	42.95
TraesCS5B01G334700.1	Wheat	endoplasmic reticulum, extracellular, vacuole	40.22	42.64
TraesCS5A01G335500.1	Wheat	extracellular, plasma membrane, vacuole	40.4	42.5
TraesCS5D01G340300.1	Wheat	extracellular, plasma membrane, vacuole	40.22	42.31
Bra013063.1-P	Field mustard	plasma membrane	22.3	42.07
GSMUA_AchrUn_...	Banana	vacuole	39.49	41.94
CDY20815	Canola	plasma membrane, plastid, vacuole	46.07	41.79
AT3G51330.1	Thale cress	extracellular, plasma membrane, vacuole	40.4	41.78
Bra036814.1-P	Field mustard	vacuole	36.75	41.61
Os09t0542100-01	Rice	plasma membrane	37.11	41.43
EER88970	Sorghum	vacuole	40.95	41.4
CDX73727	Canola	vacuole	37.29	41.38
Os04t0228000-01	Rice	extracellular, vacuole	40.04	41.32
Zm00001d014804_P001	Maize	extracellular	40.59	40.88
KRH69733	Soybean	nucleus	44.97	40.86
TraesCS7D01G524300.1	Wheat	plastid	39.12	40.84
HORVU7Hr1G118020.3	Barley	extracellular, plasma membrane, vacuole	40.04	40.78
TraesCS7A01G537200.1	Wheat	plasma membrane	39.49	40.37
Os06t0717900-02	Rice	extracellular	40.22	39.93
CDX90651	Canola	plasma membrane	38.94	39.89
CDY29013	Canola	plasma membrane	38.94	39.89
CDX78000	Canola	vacuole	36.75	39.88
CDX73728	Canola	vacuole	38.94	39.59
CDX77999	Canola	vacuole	38.76	39.55
Bra036815.1-P	Field mustard	vacuole	39.12	39.48
AT3G51360.9	Thale cress	vacuole	35.83	39.36
AT3G51350.1	Thale cress	extracellular, plasma membrane, vacuole	37.84	39.2
HORVU5Hr1G084490.1	Barley	vacuole	39.67	39.17
Bra012857.1-P	Field mustard	plasma membrane	31.63	38.36
EER89124	Sorghum	extracellular, vacuole	40.04	38.09
Zm00001d036037_P001	Maize	vacuole	40.04	38.02
AT3G51340.1	Thale cress	plasma membrane	36.56	37.74
PGSC0003DMT400056847	Potato	vacuole	26.87	36.03
PGSC0003DMT400022458	Potato	endoplasmic reticulum, extracellular, vacuole	35.1	35.75
PGSC0003DMT400037976	Potato	vacuole	18.46	24.16
PGSC0003DMT400080179	Potato	extracellular	18.65	23.34
PGSC0003DMT400027299	Potato	endoplasmic reticulum, golgi, vacuole	18.46	23.22
PGSC0003DMT400030753	Potato	extracellular	18.46	22.9
PGSC0003DMT400071953	Potato	vacuole	16.27	22.19
PGSC0003DMT400001138	Potato	plasma membrane	20.11	22.09
PGSC0003DMT400060025	Potato	endoplasmic reticulum, extracellular, vacuole	19.2	22.01
PGSC0003DMT400033805	Potato	extracellular, plasma membrane, vacuole	20.11	22.0
PGSC0003DMT400022163	Potato	cytosol, extracellular, plasma membrane	20.11	22.0
PGSC0003DMT400013994	Potato	vacuole	19.01	21.85
PGSC0003DMT400055639	Potato	endoplasmic reticulum, plasma membrane, vacuole	18.1	20.62
PGSC0003DMT400034018	Potato	golgi, plasma membrane, vacuole	17.92	20.46
PGSC0003DMT400010418	Potato	cytosol, nucleus, vacuole	17.18	16.85
PGSC0003DMT400073638	Potato	plasma membrane	18.1	15.11
PGSC0003DMT400028148	Potato	cytosol	13.89	15.05

Protein Annotations

EntrezGene:102580931	MapMan:19.4.3.1	Gene3D:2.40.70.10	InterPro:Aspartic_peptidase_A1	InterPro:Aspartic_peptidase_AS	GO:GO:0003674
GO:GO:0003824	GO:GO:0004190	GO:GO:0005575	GO:GO:0006508	GO:GO:0008150	GO:GO:0008152
GO:GO:0008233	GO:GO:0016020	GO:GO:0016021	GO:GO:0016787	GO:GO:0019538	InterPro:IPR021109
InterPro:IPR033121	UniProt:M1C1Z7	InterPro:PEPTIDASE_A1	PFAM:PF14541	PFAM:PF14543	EnsemblPlantsGene:PGSC0003DMG400022526
PGSC:PGSC0003DMG400022526	EnsemblPlants:PGSC0003DMT400058018	PRINTS:PR00792	ScanProsite:PS00141	PFscan:PS51767	PANTHER:PTHR13683
PANTHER:PTHR13683:SF232	InterPro:Pepsin-like_plant	InterPro:Peptidase_aspartic_dom_sf	SUPFAM:SSF50630	SignalP:SignalP-noTM	InterPro:TAXi_C
InterPro:TAXi_N	TMHMM:TMhelix	UniParc:UPI00029689D7	RefSeq:XP_015162344.1	SEG:seg	:

Description

Aspartic proteinase nepenthesin-1 [Source:PGSC_GENE;Acc:PGSC0003DMG400022526]

Coordinates

chr3:+:3351449..3357162

Molecular Weight (calculated)

59716.8 Da

IEP (calculated)

6.567

GRAVY (calculated)

-0.085

Length

547 amino acids

Sequence
(BLAST)

001: MDDFCCTGNF MVFVLFFALL LHNSSEAFGT FRFDIHHRYS DPVKGILDLP WLPKKRSIQY YSAWTQRDRH INRHRLTSTV DATPNSRRLT STIDPTPLPL
101: TFAGGGNKTV LLSSLGFLHY ANVTVGTPGL SFLVALDTGS DLFWLPCNCN NCAHTLLTGS GRPTDLNIYS PNTSSTSKIV PCNGTLCGQG RQCSVAQSAC
201: AYRVAYLSHN TSSSGVLVED ILHLQTDSSQ QKAFEAPITL GCGMKQTGAI LNGAAPNGLF GLGMGNISVP SVLASKGLAA NSFSLCFGAD GIGRIDFGDK
301: GSLDQGETPF NLEQTHQTYN ISLTGITVGN KNIDVDFTAI VDSGTSFTYL NDPAYKVITE NFNSQAQELR IQPMVQVPFE YCYGLRVNQT YFQILDVNLM
401: MKGGNQFYLF DPIITLSLPD GSTAYCLAVV KSGDVNIIGQ NFMTGYQVVF DREKMVLGWK PSDCYDSKES KSKTILPINK QRPTEGSGPT SGLPEDTREN
501: GNDTIRSTPD FTSSTSAGNY VTKSLTFFCQ LVAVLFFLYS HYLIISS

Best Arabidopsis Sequence Match ( AT2G17760.1 )
(BLAST)

001: MVWYSSCRIL FLGLLILLAS SWVLDRCEGF GEFGFEFHHR FSDQVVGVLP GDGLPNRDSS KYYRVMAHRD RLIRGRRLAN EDQSLVTFSD GNETVRVDAL
101: GFLHYANVTV GTPSDWFMVA LDTGSDLFWL PCDCTNCVRE LKAPGGSSLD LNIYSPNASS TSTKVPCNST LCTRGDRCAS PESDCPYQIR YLSNGTSSTG
201: VLVEDVLHLV SNDKSSKAIP ARVTFGCGQV QTGVFHDGAA PNGLFGLGLE DISVPSVLAK EGIAANSFSM CFGNDGAGRI SFGDKGSVDQ RETPLNIRQP
301: HPTYNITVTK ISVGGNTGDL EFDAVFDSGT SFTYLTDAAY TLISESFNSL ALDKRYQTTD SELPFEYCYA LSPNKDSFQY PAVNLTMKGG SSYPVYHPLV
401: VIPMKDTDVY CLAIMKIEDI SIIGQNFMTG YRVVFDREKL ILGWKESDCY TGETSARTLP SNRSSSSARP PASSFDPEAT NIPSQRPNTS TTSAAYSLSI
501: SLSLFFFSIL AIL

Arabidopsis Description

APF1Aspartyl protease family protein 1 [Source:UniProtKB/Swiss-Prot;Acc:Q8VYV9]
SUBAcon: [plasma membrane,golgi,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.