Factsheet for PGSC0003DMT400062153

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 2
vacuole 1
cytosol 2
mitochondrion 1
plasma membrane 1

Predictors

GFP

MS/MS

Papers

cytosol: 22313117

msms PMID: 22313117 doi

S Lim, K Chisholm, RH Coffin, RD Peters, KI Al-Mughrabi, G Wang-Pruski, DM Pinto

Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400018574
PGSC0003DMT400072260

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G38480.1	PGSC0003DMT400018574	AT3G56850.1	21798944
AT5G38480.1	PGSC0003DMT400072260	AT3G56850.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc02g063070.2.1	Tomato	plastid	99.62	99.62
KRH01783	Soybean	nucleus	90.38	91.44
VIT_14s0006g03230.t01	Wine grape	cytosol	91.54	90.84
GSMUA_Achr5P23090_001	Banana	cytosol	45.77	90.15
GSMUA_AchrUn_...	Banana	cytosol	45.0	89.31
GSMUA_AchrUn_...	Banana	cytosol	45.0	88.64
Bra040592.1-P	Field mustard	cytosol	88.08	87.74
CDY51543	Canola	cytosol	88.08	87.74
CDY28698	Canola	cytosol	87.69	87.36
Bra028187.1-P	Field mustard	cytosol	85.77	86.77
CDX74521	Canola	cytosol	85.77	86.77
AT5G38480.1	Thale cress	cytosol	85.0	86.67
Bra001049.1-P	Field mustard	cytosol	86.92	86.59
CDX91996	Canola	cytosol	86.92	86.59
CDY71290	Canola	cytosol	85.38	86.38
CDY01659	Canola	cytosol	85.38	86.38
CDX74252	Canola	cytosol	86.54	86.21
PGSC0003DMT400050389	Potato	cytosol	86.15	86.15
CDX67405	Canola	cytosol	85.38	86.05
Os03t0710800-01	Rice	nucleus, plasma membrane	85.77	85.77
Bra028409.1-P	Field mustard	cytosol	85.0	85.66
AT3G02520.2	Thale cress	cytosol	86.92	85.28
HORVU4Hr1G007370.1	Barley	plasma membrane	85.0	84.67
TraesCS4A01G268700.1	Wheat	cytosol	85.0	84.67
TraesCS4D01G046400.2	Wheat	golgi	85.0	84.67
TraesCS4B01G045500.2	Wheat	cytosol	85.0	84.67
KRH46909	Soybean	cytosol	84.62	84.62
PGSC0003DMT400045782	Potato	cytosol	82.31	84.25
PGSC0003DMT400079172	Potato	cytosol	83.85	84.17
PGSC0003DMT400041810	Potato	cytosol	83.08	83.72
AT5G16050.2	Thale cress	cytosol	86.15	83.58
PGSC0003DMT400060654	Potato	cytosol	81.92	83.53
TraesCS4D01G155900.1	Wheat	plastid	81.54	79.7
OQU83613	Sorghum	cytosol	81.54	79.7
Os11t0546900-01	Rice	plasma membrane	80.77	79.25
Zm00001d053090_P001	Maize	plastid	81.54	79.1
TraesCS4A01G151000.2	Wheat	cytosol	81.54	78.52
TraesCS4B01G159900.1	Wheat	cytosol	81.92	78.02
Zm00001d007446_P003	Maize	cytosol	80.77	76.09
PGSC0003DMT400041634	Potato	cytosol	70.0	73.09
PGSC0003DMT400033585	Potato	cytosol	70.38	72.62
HORVU4Hr1G043300.1	Barley	cytosol	81.92	71.0
Zm00001d048554_P001	Maize	cytosol	53.46	70.56
PGSC0003DMT400016422	Potato	cytosol	66.92	69.05
PGSC0003DMT400052619	Potato	cytosol	68.08	67.82
Zm00001d021885_P001	Maize	mitochondrion	41.92	66.06
Zm00001d033357_P001	Maize	cytosol	53.08	66.03
Zm00001d007968_P001	Maize	cytosol	52.31	65.07
Zm00001d017434_P001	Maize	cytosol	52.31	65.07
PGSC0003DMT400020200	Potato	cytosol	63.08	63.32
PGSC0003DMT400072941	Potato	cytosol	68.85	62.81
HORVU4Hr1G031770.1	Barley	cytosol, plastid	33.85	51.46
Zm00001d018856_P001	Maize	cytosol, plastid	57.31	49.34
Zm00001d039042_P001	Maize	cytosol, nucleus, plasma membrane, plastid	58.08	37.1

Protein Annotations

Gene3D:1.20.190.20	EntrezGene:102579744	InterPro:14-3-3	InterPro:14-3-3_CS	InterPro:14-3-3_dom_sf	InterPro:14-3-3_domain
MapMan:35.1	ncoils:Coil	GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	GO:GO:0019904
InterPro:IPR036815	UniProt:M1C8L3	PFAM:PF00244	EnsemblPlantsGene:PGSC0003DMG400024187	PGSC:PGSC0003DMG400024187	EnsemblPlants:PGSC0003DMT400062153
PIRSF:PIRSF000868	PRINTS:PR00305	ScanProsite:PS00796	ScanProsite:PS00797	PANTHER:PTHR18860	PANTHER:PTHR18860:SF40
SMART:SM00101	SUPFAM:SSF48445	UniParc:UPI000295FE99	RefSeq:XP_006346922.1	:	:

Description

14-3-3 protein 4 [Source:PGSC_GENE;Acc:PGSC0003DMG400024187]

Coordinates

chr2:-:21774817..21781727

Molecular Weight (calculated)

29312.3 Da

IEP (calculated)

4.372

GRAVY (calculated)

-0.526

Length

260 amino acids

Sequence
(BLAST)

001: MADSSREENV YLAKLAEQAE RYEEMIEFME KVAKTADVEE LTVEERNLLS VAYKNVIGAR RASWRIISSI EQKEESRGNE DHVNTIKEYR SKIEAELSKI
101: CDGILSLLES NLIPSASTAE SKVFYLKMKG DYHRYLAEFK TGTERKEAAE NTLLAYKSAQ DIALAELAPT HPIRLGLALN FSVFYYEILN SPDRACNLAK
201: QAFDEAISEL DTLGEESYKD STLIMQLLRD NLTLWTSDNA DDAGDDIKEA SKPESGEGQQ

Best Arabidopsis Sequence Match ( AT5G38480.1 )
(BLAST)

001: MSTREENVYM AKLAEQAERY EEMVEFMEKV AKTVDVEELS VEERNLLSVA YKNVIGARRA SWRIISSIEQ KEESKGNEDH VAIIKDYRGK IESELSKICD
101: GILNVLEAHL IPSASPAESK VFYLKMKGDY HRYLAEFKAG AERKEAAEST LVAYKSASDI ATAELAPTHP IRLGLALNFS VFYYEILNSP DRACSLAKQA
201: FDDAIAELDT LGEESYKDST LIMQLLRDNL TLWTSDMTDE AGDEIKEASK PDGAE

Arabidopsis Description

GRF3RCI1 [Source:UniProtKB/TrEMBL;Acc:A0A178UA42]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.