CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

cytosol 5
plastid 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum, nucleus
Any Predictor:	cytosol, plastid
BaCelLo:	cytosol
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	cytosol
PProwler:	plastid
WoLF PSORT:	plastid
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH25193
KRH30467

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G18190.1	KRH25193	AT5G20890.1	21798944
AT3G18190.1	KRH30467	AT5G20890.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH60476	Soybean	endoplasmic reticulum	98.69	98.69
KRH49364	Soybean	endoplasmic reticulum, nucleus	91.56	95.5
VIT_17s0000g03040.t01	Wine grape	cytosol, plastid	91.93	91.93
Bra021300.1-P	Field mustard	cytosol	89.12	89.45
CDY24438	Canola	cytosol, plastid	89.31	89.31
CDX92212	Canola	cytosol, plastid	89.31	89.31
CDY18509	Canola	cytosol	88.93	89.27
CDX95541	Canola	cytosol, plastid	88.93	88.76
Bra022292.1-P	Field mustard	cytosol, plastid	88.56	88.56
AT3G18190.1	Thale cress	cytosol, plastid	88.93	88.43
PGSC0003DMT400014441	Potato	plastid	88.56	87.57
Solyc03g118910.1.1	Tomato	plastid	88.37	87.38
GSMUA_Achr3P20910_001	Banana	cytosol	54.97	84.68
EER95631	Sorghum	cytosol	80.49	80.19
Os02t0332200-01	Rice	plasma membrane	80.11	79.96
Zm00001d027371_P001	Maize	plasma membrane	79.92	79.63
Zm00001d051742_P001	Maize	plastid	79.55	79.25
HORVU7Hr1G034140.1	Barley	plasma membrane	79.17	79.03
TraesCS7D01G164400.1	Wheat	cytosol	79.17	79.03
TraesCS7A01G426400.1	Wheat	unclear	79.17	79.03
TraesCS7B01G068100.1	Wheat	golgi, unclear	78.8	78.65
Os10t0514600-01	Rice	plasma membrane	76.92	75.79
GSMUA_Achr6P09540_001	Banana	cytosol	51.97	75.48
TraesCS6B01G391500.1	Wheat	mitochondrion	71.67	73.18
KRH33254	Soybean	cytosol	6.0	40.51
KRH31372	Soybean	nucleus	36.4	36.26
KRG97508	Soybean	cytosol, nucleus	36.4	36.26
KRH62761	Soybean	mitochondrion	8.82	34.56
KRH30467	Soybean	nucleus	33.96	34.35
KRH25193	Soybean	nucleus	33.96	34.35
KRH57568	Soybean	cytosol	6.94	33.64
KRH59103	Soybean	nucleus	32.83	32.11
KRH42973	Soybean	cytosol, endoplasmic reticulum, nucleus	32.65	31.93
KRH73433	Soybean	endoplasmic reticulum, nucleus	32.08	30.54
KRH14704	Soybean	cytosol, endoplasmic reticulum, nucleus	32.08	30.54
KRH76087	Soybean	cytosol, endoplasmic reticulum	8.82	29.94
KRH38791	Soybean	cytosol	30.58	29.42
KRH09301	Soybean	cytosol, endoplasmic reticulum, nucleus	30.21	29.06
KRG92501	Soybean	cytosol	30.21	28.9
KRH46911	Soybean	endoplasmic reticulum	26.83	26.73
KRH01781	Soybean	nucleus	26.45	26.36
KRH08492	Soybean	cytosol, endoplasmic reticulum, nucleus	26.45	25.87
KRH62778	Soybean	cytosol, endoplasmic reticulum	16.89	25.64
KRH70153	Soybean	nucleus	26.08	25.5

Protein Annotations

Gene3D:1.10.560.10	MapMan:20.3.1.4	Gene3D:3.30.260.10	Gene3D:3.50.7.10	EMBL:ACUP02004781	InterPro:Chap_CCT_delta
InterPro:Chaperone_TCP-1	InterPro:Chaperonin_TCP-1_CS	InterPro:Cpn60/TCP-1	EnsemblPlantsGene:GLYMA_08G050100	GO:GO:0000166	GO:GO:0003674
GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005829	GO:GO:0005832	GO:GO:0006457	GO:GO:0006458	GO:GO:0008150
GO:GO:0009987	GO:GO:0044183	GO:GO:0051082	GO:GO:0061077	InterPro:GROEL-like_equatorial_sf	InterPro:GroEL-like_apical_dom_sf
UniProt:I1KQG0	InterPro:IPR027409	InterPro:IPR027410	InterPro:IPR027413	EnsemblPlants:KRH41771	ProteinID:KRH41771
ProteinID:KRH41771.1	PFAM:PF00118	PRINTS:PR00304	ScanProsite:PS00750	ScanProsite:PS00995	PANTHER:PTHR11353
PANTHER:PTHR11353:SF26	SUPFAM:SSF48592	SUPFAM:SSF52029	SUPFAM:SSF54849	InterPro:TCP-1-like_intermed_sf	TIGRFAMs:TIGR02342
UniParc:UPI00023C586A	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr8:-:3929082..3933354

Molecular Weight (calculated)

57631.5 Da

IEP (calculated)

7.312

GRAVY (calculated)

0.126

Length

533 amino acids

Sequence
(BLAST)

001: MSAIAAPQHR SSKTESYVDN KRKEDIRHAN IVAARSVANA VRTSLGPKGM DKMISTSSDE VIITNDGATI LNKMQVLQPA AKMLVELSKS QDSAAGDGTT
101: TVVVIAGALL EQCLLLLSHG IHPTVVSDAL HKAAVKAVDV LTAMAVPVEL SDRDSLVKSA STSLNSKVVS QYSTLLAPLA VDAVLSVVDA PKPDMVDLRD
201: VKIVKKLGGT VDDTELVKGL VFDKKVSHAA GGPTRMENAK IAVIQFQISP PKTDIEQSIV VSDYSQMDRI LKEERSYILS MIKKIKATGC NVLLIQKSIL
301: RDAVTDLSLH YLAKAKILVI KDVERDEIEF ITKTLNCLPI ANIEHFRTEK LGYADLVEEF SLGDGKIVKI TGIKEMGKTT TVLVRGSNQL VLDEAERSLH
401: DALCVVRCLV AKRFLIAGGG APEIELSRQL GAWAKVLHGM EGYCVRAFAE ALEVIPYTLA ENAGLNPIAI VTELRNRHAQ GEINAGINVR KGQITNILEE
501: NVVQPLLVST SAITLATECV RMILKIDDIV TVR

Best Arabidopsis Sequence Match ( AT3G18190.1 )
(BLAST)

001: MAAVAAPMAS KPRGSKAESF VDNKRREDIR FANINSARAV SDAVRTSLGP KGMDKMISTA NGEVIITNDG ATILNKMEVL QPAAKMLVEL SKSQDSAAGD
101: GTTTVVVIAG ALLKECQSLL TNGIHPTVIS DSLHKACGKA IDILTAMAVP VELTDRDSLV KSASTSLNSK VVSQYSTLLA PLAVDAVLSV IDPEKPEIVD
201: LRDIKIVKKL GGTVDDTHTV KGLVFDKKVS RAAGGPTRVE NAKIAVIQFQ ISPPKTDIEQ SIVVSDYTQM DRILKEERNY ILGMIKKIKA TGCNVLLIQK
301: SILRDAVTDL SLHYLAKAKI MVIKDVERDE IEFVTKTLNC LPIANIEHFR AEKLGHADLV EEASLGDGKI LKITGIKDMG RTTSVLVRGS NQLVLDEAER
401: SLHDALCVVR CLVSKRFLIA GGGAPEIELS RQLGAWAKVL HGMEGYCVKS FAEALEVIPY TLAENAGLNP IAIVTELRNK HAQGEINAGI NVRKGQITNI
501: LEENVVQPLL VSTSAITLAT ECVRMILKID DIVTVR

Arabidopsis Description

CCT4T-complex protein 1 subunit delta [Source:UniProtKB/Swiss-Prot;Acc:Q9LV21]
SUBAcon: [plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.