CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 5
golgi 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, golgi, mitochondrion
BaCelLo:	cytosol
EpiLoc:	golgi
MultiLoc:	cytosol
Plant-mPloc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH25193
KRH30467

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G24510.1	KRH25193	AT5G20890.1	21798944
AT1G24510.1	KRH30467	AT5G20890.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG97508	Soybean	cytosol, nucleus	99.81	99.81
PGSC0003DMT400073555	Potato	cytosol	46.17	94.64
Solyc05g013990.2.1	Tomato	nucleus	94.21	94.21
EER88530	Sorghum	cytosol	91.59	91.59
Os06t0562600-01	Rice	cytosol, extracellular, plasma membrane	91.22	91.22
CDY36138	Canola	cytosol	90.84	90.84
CDY54836	Canola	cytosol	90.84	90.84
EER97880	Sorghum	cytosol	90.84	90.84
VIT_12s0028g00350.t01	Wine grape	cytosol	90.65	90.65
Bra010965.1-P	Field mustard	cytosol	90.65	90.65
CDY51593	Canola	cytosol	90.47	90.3
AT1G24510.1	Thale cress	cytosol	90.28	90.28
Bra012506.1-P	Field mustard	cytosol	90.28	90.28
GSMUA_Achr6P06770_001	Banana	cytosol	81.5	90.27
CDY03881	Canola	cytosol	90.09	90.09
CDX84951	Canola	cytosol	89.91	89.91
Bra032797.1-P	Field mustard	cytosol	89.91	89.91
GSMUA_AchrUn_...	Banana	cytosol	89.35	89.35
Zm00001d018694_P002	Maize	cytosol	90.65	88.5
TraesCS7B01G269300.2	Wheat	cytosol	88.41	88.41
TraesCS7A01G358400.1	Wheat	cytosol, unclear	88.41	88.41
TraesCS7D01G364400.2	Wheat	cytosol	88.41	88.41
HORVU7Hr1G088080.1	Barley	cytosol	88.6	88.1
TraesCS1A01G213800.1	Wheat	cytosol	65.05	88.1
TraesCS1D01G216600.1	Wheat	cytosol	88.04	88.04
Zm00001d007960_P001	Maize	cytosol	90.09	87.96
TraesCS1B01G227600.1	Wheat	cytosol	87.85	87.85
HORVU1Hr1G054530.14	Barley	cytosol	45.05	81.69
VIT_01s0137g00250.t01	Wine grape	cytosol	93.83	75.83
KRH33254	Soybean	cytosol	6.92	46.84
KRH60476	Soybean	endoplasmic reticulum	36.64	36.77
KRH41771	Soybean	endoplasmic reticulum, nucleus	36.26	36.4
KRH49364	Soybean	endoplasmic reticulum, nucleus	34.21	35.81
KRH57568	Soybean	cytosol	7.29	35.45
KRH62761	Soybean	mitochondrion	8.79	34.56
KRH30467	Soybean	nucleus	33.64	34.16
KRH25193	Soybean	nucleus	33.64	34.16
KRH59103	Soybean	nucleus	31.78	31.19
KRH42973	Soybean	cytosol, endoplasmic reticulum, nucleus	31.78	31.19
KRG92501	Soybean	cytosol	31.96	30.7
KRH76087	Soybean	cytosol, endoplasmic reticulum	8.97	30.57
KRH38791	Soybean	cytosol	31.59	30.51
KRH09301	Soybean	cytosol, endoplasmic reticulum, nucleus	31.59	30.51
KRH73433	Soybean	endoplasmic reticulum, nucleus	30.28	28.93
KRH14704	Soybean	cytosol, endoplasmic reticulum, nucleus	30.09	28.75
KRH01781	Soybean	nucleus	26.92	26.92
KRH46911	Soybean	endoplasmic reticulum	26.92	26.92
KRH08492	Soybean	cytosol, endoplasmic reticulum, nucleus	25.79	25.32
KRH70153	Soybean	nucleus	25.61	25.14
KRH62778	Soybean	cytosol, endoplasmic reticulum	16.45	25.07

Protein Annotations

Gene3D:1.10.560.10	EntrezGene:100817323	MapMan:20.3.1.5	Gene3D:3.30.260.10	Gene3D:3.50.7.10	EMBL:ACUP02007142
InterPro:Chap_CCT_epsi	InterPro:Chaperone_TCP-1	InterPro:Chaperonin_TCP-1_CS	InterPro:Cpn60/TCP-1	EnsemblPlantsGene:GLYMA_11G244700	GO:GO:0000166
GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005829	GO:GO:0005832	GO:GO:0006457	GO:GO:0006458
GO:GO:0008150	GO:GO:0009987	GO:GO:0044183	GO:GO:0051082	GO:GO:0061077	InterPro:GROEL-like_equatorial_sf
InterPro:GroEL-like_apical_dom_sf	UniProt:I1LNE6	InterPro:IPR027409	InterPro:IPR027410	InterPro:IPR027413	EnsemblPlants:KRH31372
ProteinID:KRH31372	ProteinID:KRH31372.1	PFAM:PF00118	PRINTS:PR00304	ScanProsite:PS00750	ScanProsite:PS00751
ScanProsite:PS00995	PANTHER:PTHR11353	PANTHER:PTHR11353:SF178	SUPFAM:SSF48592	SUPFAM:SSF52029	SUPFAM:SSF54849
InterPro:TCP-1-like_intermed_sf	TIGRFAMs:TIGR02343	UniParc:UPI000233D864	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr11:+:33843240..33846970

Molecular Weight (calculated)

59206.8 Da

IEP (calculated)

5.593

GRAVY (calculated)

-0.184

Length

535 amino acids

Sequence
(BLAST)

001: MALAFDEFGR PFIILKEQEQ KSRLRGLDAQ KANISAGKAV ARILRTSLGP KGMDKMLQSP DGDVTITNDG ATILDQMDVD NQIAKLMVEL SRSQDYEIGD
101: GTTGVVVMAG ALLEKAERLL ERGIHPIRIA EGYEMASRIA VEHLERVANK FEFDESNLEP LIQTCMTTLS SKIVNRCKRS LAEIAVKAVL AVADLARKDV
201: NLDLIKVEGK VGGKLEDTEL IYGIVVDKDM SHPQMPKQIE DAKIAILTCP FEPPKPKTKH KVDIDTVEKF QTLRLQEQKY FDDMVQKCKD VGATLVICQW
301: GFDDEANHLL MHRNLPAVRW VGGVELELIA IATGGRIVPR FQELSPEKLG KAGMVREKSF GTTKDRMLYI EHCANSRAVT IFIRGGNKMI IEETKRSLHD
401: ALCVARNLIR NNSIVYGGGS AEISCSIAVE AAADRYPGVE QYAIRAFGDA LEAIPMALAE NSGLQPIETL SAVKSQQIKD NNPHFGIDCN DVGTNDMREQ
501: NVFETLIGKQ QQLLLATQVV KMILKIDDVI SPSEY

Best Arabidopsis Sequence Match ( AT1G24510.1 )
(BLAST)

001: MALAFDEFGR PFIILREQDQ KTRLRGIDAQ KANIAAGKAV ARILRSSLGP KGMDKMLQGP DGDITITNDG ATILEQMDVD NQIAKLMVEL SRSQDYEIGD
101: GTTGVVVMAG ALLEQAERQL DRGIHPIRIA EGYEMASRVA VEHLERIAQK FEFDVNNYEP LVQTCMTTLS SKIVNRCKRS LAEIAVKAVL AVADLERRDV
201: NLDLIKVEGK VGGKLEDTEL IYGILIDKDM SHPQMPKQIE DAHIAILTCP FEPPKPKTKH KVDIDTVEKF ETLRKQEQQY FDEMVQKCKD VGATLVICQW
301: GFDDEANHLL MHRNLPAVRW VGGVELELIA IATGGRIVPR FQELTPEKLG KAGVVREKSF GTTKERMLYI EHCANSKAVT VFIRGGNKMM IEETKRSIHD
401: ALCVARNLIR NKSIVYGGGA AEIACSLAVD AAADKYPGVE QYAIRAFAEA LDSVPMALAE NSGLQPIETL SAVKSQQIKE NIPFYGIDCN DVGTNDMREQ
501: NVFETLIGKQ QQILLATQVV KMILKIDDVI SNSEY

Arabidopsis Description

CCT5T-complex protein 1 subunit epsilon [Source:UniProtKB/Swiss-Prot;Acc:O04450]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.