CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 6
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion
BaCelLo:	cytosol
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG97508
KRH01781
KRH14704
KRH31372
KRH46911
KRH73433

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G03960.1	KRG97508	AT1G24510.1	21798944
AT3G03960.1	KRH31372	AT1G24510.1	21798944
AT3G03960.1	KRH14704	AT3G11830.1	21798944
AT3G03960.1	KRH73433	AT3G11830.1	21798944
AT3G03960.1	KRH01781	AT5G16070.1	21798944
AT3G03960.1	KRH46911	AT5G16070.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH08492	Soybean	cytosol, endoplasmic reticulum, nucleus	97.98	97.98
VIT_08s0007g07460.t01	Wine grape	cytosol	88.81	88.81
Solyc01g088080.2.1	Tomato	nucleus	87.34	87.99
PGSC0003DMT400017609	Potato	cytosol	86.61	87.25
GSMUA_Achr3P25570_001	Banana	cytosol	86.61	86.76
GSMUA_Achr8P04580_001	Banana	cytosol	86.42	86.58
Os03t0804800-01	Rice	cytosol, extracellular, plasma membrane, plastid	86.61	86.45
EES09994	Sorghum	cytosol	86.79	86.31
Zm00001d052948_P001	Maize	cytosol, extracellular, mitochondrion	86.61	86.13
HORVU4Hr1G040660.1	Barley	cytosol	83.85	84.63
TraesCS4B01G165400.1	Wheat	cytosol	83.67	84.44
TraesCS4A01G164900.2	Wheat	cytosol	83.67	83.82
TraesCS4D01G157300.1	Wheat	cytosol	82.2	83.43
CDY29973	Canola	cytosol	82.94	83.09
Bra036422.1-P	Field mustard	cytosol	82.94	83.09
CDY21153	Canola	cytosol	82.39	82.54
Zm00001d007508_P005	Maize	cytosol	86.24	82.02
CDX92056	Canola	cytosol	82.2	81.75
AT3G03960.1	Thale cress	cytosol	82.2	81.6
Bra001094.1-P	Field mustard	cytosol	82.02	81.57
CDX74202	Canola	cytosol, plasma membrane	82.02	81.57
Bra031959.1-P	Field mustard	cytosol	81.1	81.25
CDY42892	Canola	cytosol	80.73	80.88
CDY50868	Canola	cytosol	80.73	80.88
TraesCS6B01G010900.1	Wheat	mitochondrion, plastid	76.15	78.15
CDY50320	Canola	cytosol	36.7	75.76
TraesCS6D01G008000.1	Wheat	cytosol	79.08	75.61
CDX79329	Canola	cytosol	35.41	73.11
KRH33254	Soybean	cytosol	5.14	35.44
KRH57568	Soybean	cytosol	5.87	29.09
KRH42973	Soybean	cytosol, endoplasmic reticulum, nucleus	28.07	28.07
KRH59103	Soybean	nucleus	27.89	27.89
KRH38791	Soybean	cytosol	27.71	27.26
KRH62761	Soybean	mitochondrion	6.79	27.21
KRG92501	Soybean	cytosol	27.34	26.75
KRH09301	Soybean	cytosol, endoplasmic reticulum, nucleus	27.16	26.71
KRH30467	Soybean	nucleus	25.5	26.38
KRH25193	Soybean	nucleus	25.32	26.19
KRH60476	Soybean	endoplasmic reticulum	25.5	26.08
KRH41771	Soybean	endoplasmic reticulum, nucleus	25.5	26.08
KRH49364	Soybean	endoplasmic reticulum, nucleus	24.04	25.64
KRG97508	Soybean	cytosol, nucleus	25.14	25.61
KRH31372	Soybean	nucleus	25.14	25.61
KRH76087	Soybean	cytosol, endoplasmic reticulum	7.16	24.84
KRH14704	Soybean	cytosol, endoplasmic reticulum, nucleus	25.32	24.64
KRH01781	Soybean	nucleus	24.04	24.49
KRH46911	Soybean	endoplasmic reticulum	24.04	24.49
KRH73433	Soybean	endoplasmic reticulum, nucleus	25.14	24.46
KRH62778	Soybean	cytosol, endoplasmic reticulum	14.68	22.79

Protein Annotations

Gene3D:1.10.560.10	EntrezGene:100793494	MapMan:20.3.1.8	Gene3D:3.50.7.10	EMBL:ACUP02000753	InterPro:Chap_CCT_theta
InterPro:Chaperone_TCP-1	InterPro:Chaperonin_TCP-1_CS	ncoils:Coil	InterPro:Cpn60/TCP-1	EnsemblPlantsGene:GLYMA_02G071900	GO:GO:0000166
GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005829	GO:GO:0005832	GO:GO:0006457	GO:GO:0006458
GO:GO:0008150	GO:GO:0009987	GO:GO:0044183	GO:GO:0051082	GO:GO:0061077	InterPro:GROEL-like_equatorial_sf
InterPro:GroEL-like_apical_dom_sf	UniProt:I1JD52	InterPro:IPR027409	InterPro:IPR027410	InterPro:IPR027413	EnsemblPlants:KRH70153
ProteinID:KRH70153	ProteinID:KRH70153.1	PFAM:PF00118	PRINTS:PR00304	ScanProsite:PS00750	PANTHER:PTHR11353
PANTHER:PTHR11353:SF78	SUPFAM:SSF48592	SUPFAM:SSF52029	SUPFAM:SSF54849	InterPro:TCP-1-like_intermed_sf	TIGRFAMs:TIGR02346
UniParc:UPI000233731B	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr2:-:6311976..6318662

Molecular Weight (calculated)

58593.4 Da

IEP (calculated)

5.055

GRAVY (calculated)

-0.065

Length

545 amino acids

Sequence
(BLAST)

001: MGFNIQPYGI QSMLKEGHKH LSGLDEAVLK NIDACKQLST ITRTSLGPNG MNKMVINHLD KLFVTNDAGT IVNELEVQHP AAKVLVLAGK AQQEEIGDGA
101: NLTISFAGEL LQGAEELIRM GLHPSEIISG YTKAINKTVQ ILDELVEDGS DNMDVRDKEQ VISRMKAAVA SKQFGQEDII CSLVADACIQ VCPKNPANFN
201: VDNVRVAKLL GGGLHNSTVV RGLVLKSDAV GTIKQAEKAK VAVFASGVDT SATETKGTVL IHTAEQLENY SKTEEAKVEE LIKAVADSGA KVIVSGGAVG
301: EMALHFCERY KLMVLKISSK FELRRFCRTT GSVAMLKLCQ PNPDDLGYVD SVSVQEIGGV RVTIVKNEEG GNSVATVVLR GSTDSILDDL ERAVDDGVNT
401: YKAMCRDSRF VPGAAATEIE LAKRVKDFSF KETGLDQYAI AKFAESFEMI PRTLAENAGL NAMEIISSLY AEHASGNAKV GIDLEEGICK DVSTLSIWDL
501: HVTKLFALKY AADAACTVLR VDQIIMAKPA GGPRREQPAG GMDED

Best Arabidopsis Sequence Match ( AT3G03960.1 )
(BLAST)

001: MVGMSMQPYG IQSMLKEGYR HLSGLDEAVI KNIEACKELS TITRTSLGPN GMNKMVINHL DKLFVTNDAA TIVNELEIQH PAAKLLVLAA KAQQEEIGDG
101: ANLTISFAGE LLQNAEELIR MGLHPSEIIS GYTKAVSKAV EILEQLVETG SETMDVRNKD EVISRMRAAV ASKQFGQEEI ICSLVTDACI QVCPKNPTNF
201: NVDNVRVSKL LGGGLHNSCI VRGMVLKSDA VGSIKRMEKA KVAVFAGGVD TTATETKGTV LIHSAEQLEN YAKTEEAKVE ELIKAVAESG AKVIVSGGSI
301: GEMALHFCER YKIMVLKISS KFELRRFCRT AGAVAHLKLS RPSPEDLGYV DSISVEEIGG VTVTIARNEE GGNSISTVVL RGSTDSILDD LERAVDDGVN
401: TYKAMCRDSR IVPGAAATEI ELAQRLKEYA NAEIGLDKYA ITKYAESFEF VPKTLADNAG LNAMEIIAAL YTGHGSGNTK LGIDLEEGAC KDVSETKVWD
501: LFATKLFALK YASDAACTVL RVDQIIMAKP AGGPRRDAAQ AAGAGAEED

Arabidopsis Description

CCT8T-complex protein 1 subunit theta [Source:UniProtKB/Swiss-Prot;Acc:Q94K05]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.