Factsheet for Bra017340.1-P

Subcellular Localization

min:

: max

Winner_takes_all: extracellular, endoplasmic reticulum, vacuole, plastid, cytosol, plasma membrane, golgi, nucleus

Predictor Summary:

plastid 2
cytosol 2
extracellular 3
endoplasmic reticulum 3
vacuole 3
plasma membrane 3
golgi 3
nucleus 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, nucleus, plastid, secretory
Any Predictor:	cytosol, nucleus, plastid, secretory
BaCelLo:	plastid
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	nucleus

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra001423.1-P
Bra002050.1-P
Bra005088.1-P
Bra005290.1-P
Bra005297.1-P
Bra006369.1-P
Bra009117.1-P
Bra010520.1-P
Bra011367.1-P
Bra012553.1-P
Bra012912.1-P
Bra013385.1-P
Bra013565.1-P
Bra013824.1-P
Bra017105.1-P
Bra017340.1-P
Bra017408.1-P
Bra019213.1-P
Bra019727.1-P
Bra020966.1-P
Bra021158.1-P
Bra021945.1-P
Bra022999.1-P
Bra023056.1-P
Bra023192.1-P
Bra024702.1-P
Bra024999.1-P
Bra025176.1-P
Bra025513.1-P
Bra025599.1-P
Bra026274.1-P
Bra027194.1-P
Bra027946.1-P
Bra030030.1-P
Bra030221.1-P
Bra032422.1-P
Bra034807.1-P
Bra037049.1-P
Bra038682.1-P
Bra038753.1-P
Bra039922.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G25980.1	Bra030030.1-P	AT1G24290.1	20706207
AT3G25980.1	Bra024702.1-P	AT1G26630.1	20706207
AT3G25980.1	Bra027946.1-P	AT1G56290.1	20706207
AT3G25980.1	Bra019727.1-P	AT1G62390.1	20706207
AT3G25980.1	Bra002050.1-P	AT2G17350.1	20706207
AT3G25980.1	Bra030221.1-P	AT2G22640.1	25262777
AT3G25980.1	Bra039922.1-P	AT2G22640.1	25262777
AT3G25980.1	Bra021945.1-P	AT2G35050.1	20706207
AT3G25980.1	Bra022999.1-P	AT2G35050.1	20706207
AT3G25980.1	Bra005297.1-P	AT2G36130.1	20706207
AT3G25980.1	Bra005290.1-P	AT2G36230.1	20706207
AT3G25980.1	Bra023056.1-P	AT2G36230.1	20706207
AT3G25980.1	Bra005088.1-P	AT2G38730.1	20706207
AT3G25980.1	Bra017105.1-P	AT2G38730.1	20706207
AT3G25980.1	Bra006369.1-P	AT3G11270.1	20706207
AT3G25980.1	Bra017408.1-P	AT3G11270.1	20706207
AT3G25980.1	Bra032422.1-P	AT3G11270.1	20706207
AT3G25980.1	Bra001423.1-P	AT3G11830.1	20706207
AT3G25980.1	Bra034807.1-P	AT3G11830.1	20706207
AT3G25980.1	Bra038682.1-P	AT3G11830.1	20706207
AT3G25980.1	Bra021158.1-P	AT3G16060.1	20706207
AT3G25980.1	Bra023192.1-P	AT3G16060.1	20706207
AT3G25980.1	Bra027194.1-P	AT3G16060.1	20706207
AT3G25980.1	Bra017340.1-P	AT3G25980.1	25262777
AT3G25980.1	Bra025176.1-P	AT3G25980.1	25262777
AT3G25980.1	Bra012912.1-P	AT3G49990.1	20706207
AT3G25980.1	Bra012553.1-P	AT4G19210.1	20706207
AT3G25980.1	Bra013385.1-P	AT4G19210.1	20706207
AT3G25980.1	Bra020966.1-P	AT4G19210.1	20706207
AT3G25980.1	Bra013565.1-P	AT4G21860.1	20706207
AT3G25980.1	Bra038753.1-P	AT4G21860.1	20706207
AT3G25980.1	Bra013824.1-P	AT4G24680.1	20706207
AT3G25980.1	Bra019213.1-P	AT4G24680.1	20706207
AT3G25980.1	Bra026274.1-P	AT4G28230.1	20706207
AT3G25980.1	Bra011367.1-P	AT4G32650.1	20706207
AT3G25980.1	Bra037049.1-P	AT4G32650.1	20706207
AT3G25980.1	Bra010520.1-P	AT4G35740.1	20706207
AT3G25980.1	Bra025599.1-P	AT4G38440.1	20706207
AT3G25980.1	Bra009117.1-P	AT5G05510.1	25262777
AT3G25980.1	Bra025513.1-P	AT5G41190.1	20706207
AT3G25980.1	Bra024999.1-P	AT5G46750.1	20706207

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY04187	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	100.0	100.0
CDY39177	Canola	cytosol	99.04	99.04
AT3G25980.1	Thale cress	cytosol	95.22	95.22
KRG97470	Soybean	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, nucleus, plasma membrane, plastid, vacuole	89.95	90.82
KRH31421	Soybean	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, nucleus, plasma membrane, plastid, vacuole	89.47	90.34
VIT_01s0010g02430.t01	Wine grape	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, nucleus, plasma membrane, plastid, vacuole	89.0	89.86
GSMUA_Achr6P21190_001	Banana	cytosol, nucleus, plastid	35.41	89.16
PGSC0003DMT400045025	Potato	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, nucleus, plasma membrane, plastid, vacuole	87.56	87.98
Solyc05g014370.2.1	Tomato	mitochondrion	87.08	87.5
Os04t0486500-01	Rice	mitochondrion	84.69	85.51
EES12410	Sorghum	mitochondrion	84.21	85.02
TraesCS2B01G363300.1	Wheat	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	82.3	83.09
TraesCS2A01G320000.1	Wheat	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, nucleus, plasma membrane, plastid, vacuole	81.82	82.61
TraesCS2D01G343300.1	Wheat	cytosol, endoplasmic reticulum, extracellular, golgi, nucleus, plasma membrane, plastid, vacuole	81.34	82.13
Zm00001d025721_P001	Maize	mitochondrion	80.38	78.14
KRH08024	Soybean	cytosol	37.8	73.83
Bra025176.1-P	Field mustard	cytosol	33.01	72.63
HORVU2Hr1G084050.1	Barley	plastid	82.3	63.24
Zm00001d003249_P001	Maize	mitochondrion	72.73	31.93

Protein Annotations

EnsemblPlants:Bra017340.1	EnsemblPlants:Bra017340.1-P	EnsemblPlantsGene:Bra017340	Gene3D:3.30.900.10	GO:GO:0007049	GO:GO:0007094
GO:GO:0008150	GO:GO:0009987	GO:GO:0016043	InterPro:HORMA_dom	InterPro:HORMA_dom_sf	InterPro:IPR003511
InterPro:IPR036570	InterPro:Mad2	PANTHER:PTHR11842	PANTHER:PTHR11842:SF11	PFAM:PF02301	PFscan:PS50815
SUPFAM:SSF56019	UniParc:UPI00025421CB	UniProt:M4DLF9	MapMan:13.3.5.5.4	:	:

Description

AT3G25980 (E=2e-112) | mitotic spindle checkpoint protein, putative (MAD2)

Coordinates

chrA09:-:14386231..14387288

Molecular Weight (calculated)

23670.3 Da

IEP (calculated)

4.602

GRAVY (calculated)

-0.138

Length

209 amino acids

Sequence
(BLAST)

001: MASKTAAAKD IITLHGSAAI VSEFFCYAAN SILYNRAVYP EESFAKVKKY GLPMLLTQDE SVKSFLSNLT SQISEWLEAG KLQRVVLVIM SKATGEVLER
101: WNFRIETDSE VVEKGVSREK SDKEIMREIQ AIMRQVASSI TYLPCLDEPC VFDVLAYTDT DVAVPFTWVE SDPKLIANPQ MVKLHAFDTK IHKVDTLVSY
201: KNDEWDEEE

Best Arabidopsis Sequence Match ( AT3G25980.1 )
(BLAST)

001: MASKTAAAKD IITLHGSAAI VSEFFCYAAN SILYNRAVYP EESFVKVKKY GLPMLLIEDE SVKSFMSNLT SQISEWLEAG KLQRVVLVIM SKATGEVLER
101: WNFRIETDNE VVDKGVSREK SDKEIMREIQ AIMRQVASSV TYLPCLDETC VFDVLAYTDT DVAVPFTWIE SDPKLIANPQ MVKLHGFDTK IHKVDTLVSY
201: KNDEWDEEE

Arabidopsis Description

MAD2Mitotic spindle checkpoint protein MAD2 [Source:UniProtKB/Swiss-Prot;Acc:Q9LU93]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.