CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 2
cytosol 3
mitochondrion 3
nucleus 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH24006
KRH27971
KRH29310
KRH77487

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G04390.1	KRH24006	AT3G48150.1	21798944
AT2G04390.1	KRH27971	AT3G48150.1	21798944
AT2G04390.1	KRH29310	AT3G48150.1	21798944
AT2G04390.1	KRH77487	AT3G48150.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH50389	Soybean	cytosol	91.11	96.09
KRH30810	Soybean	cytosol, endoplasmic reticulum	59.26	90.91
KRH72240	Soybean	endoplasmic reticulum	88.15	85.0
KRH32794	Soybean	cytosol	87.41	84.89
CDY47259	Canola	cytosol	83.7	82.48
Bra005887.1-P	Field mustard	cytosol	83.7	82.48
CDY04927	Canola	cytosol	82.96	82.35
CDX80997	Canola	cytosol	82.96	81.75
VIT_08s0007g02620.t01	Wine grape	cytosol	85.19	81.56
Bra009431.1-P	Field mustard	cytosol	81.48	80.88
CDX70203	Canola	cytosol	81.48	80.88
CDY44878	Canola	cytosol	81.48	80.88
Bra005820.1-P	Field mustard	cytosol	80.74	80.74
CDY11309	Canola	cytosol	80.74	80.74
AT2G04390.1	Thale cress	cytosol	83.7	80.14
Bra002983.1-P	Field mustard	cytosol	66.67	79.65
CDY19565	Canola	cytosol	66.67	79.65
CDY25845	Canola	cytosol	66.67	79.65
CDY48004	Canola	cytosol	66.67	79.65
AT5G04800.2	Thale cress	cytosol	82.96	79.43
AT2G05220.1	Thale cress	cytosol	82.22	79.29
AT3G10610.1	Thale cress	cytosol	80.74	77.86
CDY00960	Canola	cytosol	80.0	77.7
Solyc11g006690.1.1	Tomato	nucleus	82.22	77.08
Solyc05g055230.1.1	Tomato	nucleus	81.48	76.39
PGSC0003DMT400072583	Potato	cytosol	79.26	70.86
Os03t0109500-00	Rice	plasma membrane	74.07	69.44
GSMUA_Achr5P10190_001	Banana	cytosol	24.44	68.75
TraesCS1B01G174800.1	Wheat	golgi, unclear	71.85	67.83
Zm00001d045000_P001	Maize	mitochondrion	71.11	67.61
Os10t0411800-01	Rice	nucleus	70.37	67.38
TraesCS1A01G159100.1	Wheat	nucleus	71.11	67.13
TraesCS1D01G156400.2	Wheat	golgi	71.11	67.13
EER88019	Sorghum	cytosol	70.37	66.9
Zm00001d037111_P001	Maize	plastid	70.37	66.9
Bra028778.1-P	Field mustard	cytosol	81.48	61.45
CDY14138	Canola	nucleus	82.96	60.54
HORVU1Hr1G042220.1	Barley	nucleus	71.11	54.55

Protein Annotations

Gene3D:1.10.60.20	EntrezGene:100527036	MapMan:17.1.3.1.19	EMBL:ACUP02012200	EMBL:BT091845	UniProt:C6T336
EnsemblPlantsGene:GLYMA_20G017700	GO:GO:0000028	GO:GO:0003674	GO:GO:0003735	GO:GO:0005198	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005829	GO:GO:0005840	GO:GO:0006412
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016043	GO:GO:0019538
GO:GO:0022627	InterPro:IPR036401	EnsemblPlants:KRG89343	ProteinID:KRG89343	ProteinID:KRG89343.1	HAMAP:MF_00511
PFAM:PF00833	ScanProsite:PS00712	PANTHER:PTHR10732	PANTHER:PTHR10732:SF2	InterPro:RPS17e-like_sf	InterPro:Ribosomal_S17e
InterPro:Ribosomal_S17e_CS	SUPFAM:SSF116820	UniParc:UPI0001B15314	:	:	:

Description

hypothetical protein

Coordinates

chr20:-:1738235..1740132

Molecular Weight (calculated)

15733.2 Da

IEP (calculated)

10.639

GRAVY (calculated)

-0.585

Length

135 amino acids

Sequence
(BLAST)

001: MGRVRTKTVK KSSRQVIERY YSRMTLDFHT NKKLLEEVAI IPSKRLRNKI AGFSTHLMKR IQKGPVRGIS LKLQEEERER RMDFVPDVSA INTDHIEVDK
101: ETLDMLHSLG INDIPGITQV DPVPVQQAFP FTRRY

Best Arabidopsis Sequence Match ( AT5G04800.1 )
(BLAST)

001: MGRVRTKTVK KSSRQVIEKY YSRMTLDFHT NKKILEEVAI IPSKRLRNKI AGFSTHLMKR IQKGPVRGIS LKLQEEERER RMDFVPDESA IKTDEIKVDK
101: ETLEMLASLG MSDTPGISAV EPQQAMAPIA AFGGGRAPRR Y

Arabidopsis Description

RPS17D40S ribosomal protein S17-4 [Source:UniProtKB/Swiss-Prot;Acc:Q9LZ17]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.