CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 1
cytosol 4
plastid 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164
endoplasmic reticulum: 28499913
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG92440
KRG98291
KRH10001
KRH16959
KRH23330
KRH25284
KRH30312
KRH30384
KRH34357
KRH39137
KRH41763
KRH44167
KRH47141
KRH48568
KRH53667
KRH60461
KRH64261

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G09630.1	KRH39137	AT1G17970.1	21798944
AT3G09630.1	KRH41763	AT1G17970.1	21798944
AT3G09630.1	KRH48568	AT1G17970.1	21798944
AT3G09630.1	KRH60461	AT1G17970.1	21798944
AT3G09630.1	KRH10001	AT3G13720.1	21798944
AT3G09630.1	KRH23330	AT3G13720.1	21798944
AT3G09630.1	KRH44167	AT3G13720.1	21798944
AT3G09630.1	KRH47141	AT3G13720.1	21798944
AT3G09630.1	KRG92440	AT3G22960.1	21798944
AT3G09630.1	KRH34357	AT3G22960.1	21798944
AT5G02870.1	KRH16959	AT3G49580.1	21798944
AT5G02870.1	KRH53667	AT3G49580.1	21798944
AT5G02870.1	KRH64261	AT3G49580.1	21798944
AT5G02870.1	KRH25284	AT5G22310.1	21798944
AT5G02870.1	KRH30384	AT5G22310.1	21798944
AT5G02870.1	KRG98291	AT5G58720.1	21798944
AT5G02870.1	KRH30312	AT5G58720.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH32387	Soybean	nucleus	97.53	97.53
VIT_06s0004g00360.t01	Wine grape	cytosol	88.89	88.89
VIT_08s0040g03200.t01	Wine grape	cytosol	88.64	88.42
AT3G09630.1	Thale cress	cytosol	83.95	83.74
Bra009560.1-P	Field mustard	cytosol	83.7	83.5
CDX80842	Canola	cytosol	83.46	83.25
CDX70339	Canola	cytosol	83.46	83.25
AT5G02870.1	Thale cress	cytosol	83.21	82.8
CDY10418	Canola	cytosol	83.21	82.8
Solyc09g007250.2.1	Tomato	nucleus	82.96	82.76
Bra005749.1-P	Field mustard	cytosol	82.96	82.76
CDY22192	Canola	cytosol	79.75	82.61
CDY41301	Canola	cytosol	79.75	82.61
PGSC0003DMT400071725	Potato	cytosol	82.72	82.51
CDY11231	Canola	cytosol	79.51	82.14
KRH67633	Soybean	endoplasmic reticulum, mitochondrion	80.49	82.12
PGSC0003DMT400028707	Potato	cytosol	79.26	82.1
KRG95915	Soybean	endoplasmic reticulum	87.16	82.09
PGSC0003DMT400045381	Potato	cytosol	82.47	82.06
CDY44721	Canola	cytosol, extracellular	81.98	81.77
Solyc10g084350.1.1	Tomato	nucleus	41.48	81.55
CDY41596	Canola	cytosol	81.48	81.28
Bra029780.1-P	Field mustard	cytosol	80.99	80.79
CDX73989	Canola	cytosol	80.0	79.8
Bra001302.1-P	Field mustard	cytosol	79.75	79.56
Bra034075.1-P	Field mustard	cytosol	75.56	78.66
Solyc10g086010.1.1	Tomato	extracellular	82.72	77.01
KRG96019	Soybean	endoplasmic reticulum, extracellular	15.56	70.0
Bra006658.1-P	Field mustard	plastid	13.09	53.54
CDY07934	Canola	cytosol, endoplasmic reticulum, mitochondrion, plasma membrane, plastid	78.27	40.69
KRG89783	Soybean	plastid	4.44	18.75

Protein Annotations

EntrezGene:100779764	MapMan:17.1.2.1.2	Gene3D:3.40.1370.10	EMBL:ACUP02008206	ncoils:Coil	EnsemblPlantsGene:GLYMA_13G136400
GO:GO:0002181	GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0003735	GO:GO:0005198
GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005829
GO:GO:0005840	GO:GO:0006412	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987
GO:GO:0019538	GO:GO:0022625	UniProt:I1LZ03	InterPro:IPR023574	EnsemblPlants:KRH19796	ProteinID:KRH19796
ProteinID:KRH19796.1	PFAM:PF00573	PFAM:PF14374	ScanProsite:PS00939	PANTHER:PTHR19431	PANTHER:PTHR19431:SF1
InterPro:Ribos_L4_C_dom	InterPro:Ribosomal_L4/L1e	InterPro:Ribosomal_L4/L1e_euk/arc_CS	InterPro:Ribosomal_L4_dom_sf	SUPFAM:SSF52166	UniParc:UPI000233AC4E
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr13:-:24904402..24907099

Molecular Weight (calculated)

44664.3 Da

IEP (calculated)

11.172

GRAVY (calculated)

-0.463

Length

405 amino acids

Sequence
(BLAST)

001: MAAAARPLVT VQALDGDMAT DASATVPIPD VMKASIRPDI VNFVHSNISK NSRQPYAVSR RAGHQTSAES WGTGRAVSRI PRVPGGGTHR AGQGAFGNMC
101: RGGRMFAPTK IWRRWHRKIN VNQKRYAVVS AIAASAIPSL VLARGHRIES VPELPLVVSD SAEGVEKTKE AIKVLKQIGA FPDAEKAKDS RSIRPGKGKM
201: RNRRYISRRG PLVVYGTEGA KAVKAFRNVP GVEVANVERL NLLKLAPGGH LGRFVIWTKS AFEKLDSIYG SFDKPSEKKK GYLLPRSKMV NSDLSRIINS
301: DEVQSVVRPI KKDVKRATLK KNPLKNLNSM LKLNPYAKTA KRMALLAEAQ RVKVKKEKLD KKRKTVSKEE ASAIKAAGKA WYHTMVSDSD YTEFDNFSKW
401: LGVSQ

Best Arabidopsis Sequence Match ( AT3G09630.1 )
(BLAST)

001: MAAAAARPLV TIQTLDGDMS TDQSSTVVLP DVMTAPVRPD IVNFVHAQIS NNSRQPYAVS KKAGHQTSAE SWGTGRAVSR IPRVPGGGTH RAGQAAFGNM
101: CRGGRMFAPT KIWRRWHRRV NVNMKRHAIV SAIAATAVPA LVMARGHKIE NVPEMPLVVS DSAEAVEKTS AAIKVLKQIG AYDDAEKAKN SIGIRPGKGK
201: MRNRRYISRK GPLVVYGTEG SKIVKAFRNL PGVELCHVER LNLLKLAPGG HLGRFVIWTK SAFEKLESIY GSFEKPSEKK KGYVLPRAKM VNADLARIIN
301: SDEIQSVVNP IKKDAKRAVL KKNPLKNLNV MLKLNPYAKT AKRMSLLAEA QRVKAKKEKL AKKRKTVTKE EALAIKAAGK SWYKTMISDS DYTEFDNFTK
401: WLGASQ

Arabidopsis Description

RPL4A60S ribosomal protein L4-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9SF40]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.