CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion, endoplasmic reticulum

Predictor Summary:

nucleus 1
cytosol 3
plastid 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 28499913
mitochondrion: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG92440
KRG98291
KRH10001
KRH16959
KRH23330
KRH25284
KRH30312
KRH30384
KRH34357
KRH39137
KRH41763
KRH44167
KRH47141
KRH48568
KRH53667
KRH60461
KRH64261

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G09630.1	KRH39137	AT1G17970.1	21798944
AT3G09630.1	KRH41763	AT1G17970.1	21798944
AT3G09630.1	KRH48568	AT1G17970.1	21798944
AT3G09630.1	KRH60461	AT1G17970.1	21798944
AT3G09630.1	KRH10001	AT3G13720.1	21798944
AT3G09630.1	KRH23330	AT3G13720.1	21798944
AT3G09630.1	KRH44167	AT3G13720.1	21798944
AT3G09630.1	KRH47141	AT3G13720.1	21798944
AT3G09630.1	KRG92440	AT3G22960.1	21798944
AT3G09630.1	KRH34357	AT3G22960.1	21798944
AT5G02870.1	KRH16959	AT3G49580.1	21798944
AT5G02870.1	KRH53667	AT3G49580.1	21798944
AT5G02870.1	KRH64261	AT3G49580.1	21798944
AT5G02870.1	KRH25284	AT5G22310.1	21798944
AT5G02870.1	KRH30384	AT5G22310.1	21798944
AT5G02870.1	KRG98291	AT5G58720.1	21798944
AT5G02870.1	KRH30312	AT5G58720.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG95915	Soybean	endoplasmic reticulum	90.68	83.72
KRH32387	Soybean	nucleus	82.12	80.49
KRH19796	Soybean	nucleus	82.12	80.49
VIT_08s0040g03200.t01	Wine grape	cytosol	80.1	78.33
VIT_06s0004g00360.t01	Wine grape	cytosol	79.85	78.27
PGSC0003DMT400028707	Potato	cytosol	73.8	74.94
AT3G09630.1	Thale cress	cytosol	75.57	73.89
Solyc09g007250.2.1	Tomato	nucleus	75.57	73.89
PGSC0003DMT400071725	Potato	cytosol	75.31	73.65
PGSC0003DMT400045381	Potato	cytosol	75.06	73.22
Bra009560.1-P	Field mustard	cytosol	74.81	73.15
CDX70339	Canola	cytosol	74.81	73.15
CDX80842	Canola	cytosol	74.06	72.41
AT5G02870.1	Thale cress	cytosol	74.06	72.24
CDY10418	Canola	cytosol	74.06	72.24
Bra005749.1-P	Field mustard	cytosol	73.55	71.92
CDY41301	Canola	cytosol	70.78	71.87
CDY22192	Canola	cytosol	70.78	71.87
Solyc10g084350.1.1	Tomato	nucleus	37.28	71.84
CDY11231	Canola	cytosol	70.03	70.92
CDY41596	Canola	cytosol	72.29	70.69
CDY44721	Canola	cytosol, extracellular	72.29	70.69
CDX73989	Canola	cytosol	71.54	69.95
Bra001302.1-P	Field mustard	cytosol	71.28	69.7
Bra029780.1-P	Field mustard	cytosol	71.28	69.7
Solyc10g086010.1.1	Tomato	extracellular	75.06	68.51
Bra034075.1-P	Field mustard	cytosol	66.5	67.87
KRG96019	Soybean	endoplasmic reticulum, extracellular	13.35	58.89
Bra006658.1-P	Field mustard	plastid	12.09	48.48
CDY07934	Canola	cytosol, endoplasmic reticulum, mitochondrion, plasma membrane, plastid	71.03	36.2
KRG89783	Soybean	plastid	3.53	14.58

Protein Annotations

MapMan:17.1.2.1.2	Gene3D:3.40.1370.10	EMBL:ACUP02002069	EnsemblPlantsGene:GLYMA_03G177400	GO:GO:0002181	GO:GO:0003674
GO:GO:0003676	GO:GO:0003723	GO:GO:0003735	GO:GO:0005198	GO:GO:0005488	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005829	GO:GO:0005840	GO:GO:0006412
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0019538	GO:GO:0022625
UniProt:I1JPI7	InterPro:IPR023574	EnsemblPlants:KRH67633	ProteinID:KRH67633	ProteinID:KRH67633.1	PFAM:PF00573
PFAM:PF14374	ScanProsite:PS00939	PANTHER:PTHR19431	PANTHER:PTHR19431:SF1	InterPro:Ribos_L4_C_dom	InterPro:Ribosomal_L4/L1e
InterPro:Ribosomal_L4/L1e_euk/arc_CS	InterPro:Ribosomal_L4_dom_sf	SUPFAM:SSF52166	UniParc:UPI00023C2456	SEG:seg	:

Description

hypothetical protein

Coordinates

chr3:+:38995717..38997703

Molecular Weight (calculated)

43677.7 Da

IEP (calculated)

11.333

GRAVY (calculated)

-0.329

Length

397 amino acids

Sequence
(BLAST)

001: MAVAAARPIV SVQTLEGDAT PTVPLPDVMK ASIRPDIVNF VHSNISRNSR QPYAVSKRAG HQTSAESWGT GRAVSRIPRV PGGGTHRAGQ GTFGNMCRGG
101: RMFAPTKIWR RWHRKINVQQ KRHAVVSAIA ASAIPSLVQA RGHRIESVPE LPLVVSDTVE SVEKSKEAVK ILQKIGAFTD AEKAKLSHGI RPGKGKMRNR
201: RYISGKGPLI VYGTEGAKAI KAFRNIPGVE VANVDRLNLL KLAPGGHLGR FIIWTKSAFE KLDSIYGSFD KASEKKNGYL LPRPKMINSD LARIINSDEV
301: QSVVRPIKKD VKRAPLKKNP LTNLNAMLKL NPYAKTAKRM ALLAEKQRIV AKKEKLHKKR NIVSKEEASA IKAAGKSVGK YLCFNLKFIN IYINSIL

Best Arabidopsis Sequence Match ( AT3G09630.1 )
(BLAST)

001: MAAAAARPLV TIQTLDGDMS TDQSSTVVLP DVMTAPVRPD IVNFVHAQIS NNSRQPYAVS KKAGHQTSAE SWGTGRAVSR IPRVPGGGTH RAGQAAFGNM
101: CRGGRMFAPT KIWRRWHRRV NVNMKRHAIV SAIAATAVPA LVMARGHKIE NVPEMPLVVS DSAEAVEKTS AAIKVLKQIG AYDDAEKAKN SIGIRPGKGK
201: MRNRRYISRK GPLVVYGTEG SKIVKAFRNL PGVELCHVER LNLLKLAPGG HLGRFVIWTK SAFEKLESIY GSFEKPSEKK KGYVLPRAKM VNADLARIIN
301: SDEIQSVVNP IKKDAKRAVL KKNPLKNLNV MLKLNPYAKT AKRMSLLAEA QRVKAKKEKL AKKRKTVTKE EALAIKAAGK SWYKTMISDS DYTEFDNFTK
401: WLGASQ

Arabidopsis Description

RPL4A60S ribosomal protein L4-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9SF40]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.