CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 3
extracellular 3
endoplasmic reticulum 4
vacuole 3
plasma membrane 4
golgi 3
mitochondrion 1
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	mitochondrion, nucleus, plastid, secretory
BaCelLo:	nucleus
EpiLoc:	nucleus
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	secretory
TargetP:	mitochondrion
WoLF PSORT:	plastid
YLoc:	plasma membrane

endoplasmic reticulum: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH03001
KRH13717
KRH52586
KRH54987
KRH58482
KRH61926
KRH63129
KRH72344

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G22360.1	KRH13717	AT1G16500.1	21798944
AT5G22360.1	KRH52586	AT1G22920.1	21798944
AT5G22360.1	KRH61926	AT1G22920.1	21798944
AT5G22360.1	KRH03001	AT2G24100.1	21798944
AT5G22360.1	KRH54987	AT2G24100.1	21798944
AT5G22360.1	KRH58482	AT2G24100.1	21798944
AT5G22360.1	KRH63129	AT2G24100.1	21798944
AT5G22360.1	KRH72344	AT2G24100.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH25265	Soybean	plasma membrane	98.64	98.64
VIT_06s0080g00800.t01	Wine grape	endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	89.55	89.55
Zm00001d052756_P001	Maize	endoplasmic reticulum	74.55	84.97
AT5G22360.1	Thale cress	plasma membrane	85.0	84.62
Bra020187.1-P	Field mustard	endoplasmic reticulum	85.0	84.62
CDY09133	Canola	endoplasmic reticulum	85.0	84.62
GSMUA_Achr10P...	Banana	endoplasmic reticulum, plasma membrane, plastid	83.64	84.4
GSMUA_Achr11P...	Banana	endoplasmic reticulum	83.18	83.94
Bra006623.1-P	Field mustard	endoplasmic reticulum, plasma membrane	84.09	83.71
CDX70999	Canola	endoplasmic reticulum	84.09	83.71
CDX88799	Canola	plasma membrane	84.09	83.71
EES13179	Sorghum	endoplasmic reticulum, plasma membrane	82.73	81.98
TraesCS7D01G324800.1	Wheat	plasma membrane	82.27	81.53
TraesCS7A01G328000.1	Wheat	plasma membrane	82.27	81.53
TraesCS7B01G228700.1	Wheat	plasma membrane	82.27	81.53
HORVU7Hr1G078500.1	Barley	plasma membrane	81.82	81.08
Os10t0154000-01	Rice	plasma membrane	81.36	79.2
PGSC0003DMT400015063	Potato	plastid	80.0	77.19
Solyc06g074250.2.1	Tomato	endoplasmic reticulum, plasma membrane	89.09	75.1
KRH73537	Soybean	endoplasmic reticulum	53.18	53.42
KRH14588	Soybean	endoplasmic reticulum	52.73	52.97
KRH20159	Soybean	plastid	52.27	51.34
KRH03646	Soybean	plasma membrane	52.27	51.11
KRH46773	Soybean	endoplasmic reticulum	37.73	37.56
KRG99810	Soybean	endoplasmic reticulum	37.27	37.1
KRG90219	Soybean	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	36.82	36.65
KRH33448	Soybean	extracellular, golgi	10.45	36.51
KRH36720	Soybean	endoplasmic reticulum	34.55	34.39
KRH12042	Soybean	cytosol	34.09	33.94
KRH11727	Soybean	cytosol	34.09	33.94
KRH02246	Soybean	cytosol	33.64	33.48
KRH36005	Soybean	endoplasmic reticulum	35.91	33.19
KRH50864	Soybean	cytosol	33.18	33.03
KRH37118	Soybean	cytosol	33.18	32.74
KRG89410	Soybean	cytosol	32.73	32.0
KRH50475	Soybean	endoplasmic reticulum	32.27	31.56
KRH33450	Soybean	cytosol	23.64	29.55
KRG90750	Soybean	cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, plastid, vacuole	31.82	27.67
KRG99807	Soybean	cytosol	27.73	27.6
KRH57708	Soybean	plastid	10.91	20.34

Protein Annotations

Gene3D:1.20.5.110	EntrezGene:100780787	MapMan:22.7.5.1	Gene3D:3.30.450.50	EMBL:ACUP02007004	EMBL:BT098899
UniProt:C6TME8	EnsemblPlantsGene:GLYMA_11G181900	GO:GO:0000149	GO:GO:0003674	GO:GO:0005484	GO:GO:0005488
GO:GO:0005515	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006810
GO:GO:0006887	GO:GO:0006906	GO:GO:0008150	GO:GO:0009987	GO:GO:0016020	GO:GO:0016021
GO:GO:0016043	GO:GO:0016192	GO:GO:0031201	InterPro:IPR001388	InterPro:IPR010908	EnsemblPlants:KRH30399
ProteinID:KRH30399	ProteinID:KRH30399.1	InterPro:Longin-like_dom_sf	InterPro:Longin_dom	PFAM:PF00957	PFAM:PF13774
PRINTS:PR00219	ScanProsite:PS00417	PFscan:PS50859	PFscan:PS50892	PANTHER:PTHR21136	PANTHER:PTHR21136:SF165
SMART:SM01270	SUPFAM:SSF58038	SUPFAM:SSF64356	SignalP:SignalP-noTM	InterPro:Synaptobrevin	TMHMM:TMhelix
UniParc:UPI0001B1722B	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr11:-:24902697..24906840

Molecular Weight (calculated)

25129.7 Da

IEP (calculated)

9.223

GRAVY (calculated)

0.070

Length

220 amino acids

Sequence
(BLAST)

001: MAIFYALVAR GTVVLAEFSA VTGNTGAVSR RILEKLPAES DSRLCFSQDR YIFHILRSDG ITYVCMANDT FGRRIPFSYL EDIQMRFMKN YSRVANYAPA
101: YAMNDEFSRV LHQQMEFFSS NASADTLNRV RGEVGEIRTI MVDNIEKILE RGDRIELLVD KTATMQDSAF HFRKQSKRLR RALWMKNFKL LALLTCFIVI
201: VLYLIIAACC GGISLPSCRS

Best Arabidopsis Sequence Match ( AT5G22360.1 )
(BLAST)

001: MAIVYAVVAR GTVVLAEFSA VTGNTGAVVR RILEKLSPEI SDERLCFSQD RYIFHILRSD GLTFLCMAND TFGRRVPFSY LEEIHMRFMK NYGKVAHNAP
101: AYAMNDEFSR VLHQQMEFFS SNPSVDTLNR VRGEVSEIRS VMVENIEKIM ERGDRIELLV DKTATMQDSS FHFRKQSKRL RRALWMKNAK LLVLLTCLIV
201: FLLYIIIASF CGGITLPSCR S

Arabidopsis Description

VAMP714Vesicle-associated membrane protein 714 [Source:UniProtKB/Swiss-Prot;Acc:Q9FMR5]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.