CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol, nucleus

Predictor Summary:

nucleus 1
plastid 2
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG97189
KRH06008
KRH10835
KRH17522
KRH20483
KRH21480
KRH31595
KRH50009
KRH51611
KRH60933
KRH68916

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G49250.1	KRH06008	AT1G20630.1	21798944
AT3G49250.1	KRH17522	AT1G20630.1	21798944
AT3G49250.1	KRH51611	AT1G20630.1	21798944
AT3G49250.1	KRH60933	AT1G20630.1	21798944
AT3G49250.1	KRG97189	AT3G61790.1	21798944
AT3G49250.1	KRH68916	AT3G61790.1	21798944
AT3G49250.1	KRH10835	AT4G21670.1	18541146
AT3G49250.1	KRH20483	AT4G21670.1	18541146
AT3G49250.1	KRH21480	AT4G21670.1	18541146
AT3G49250.1	KRH50009	AT4G21670.1	18541146
AT3G49250.1	KRH31595	AT4G35160.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH53504	Soybean	plastid	69.27	74.02
Solyc03g071800.1.1	Tomato	cytosol	22.94	61.73
VIT_16s0098g01820.t01	Wine grape	cytosol	57.8	58.47
Solyc03g071820.1.1	Tomato	cytosol	19.5	55.92
Solyc11g032150.1.1	Tomato	cytosol, extracellular, nucleus	9.17	55.56
GSMUA_Achr10P...	Banana	cytosol	42.43	54.25
KRG97196	Soybean	cytosol	37.16	53.47
AT3G49250.1	Thale cress	cytosol	48.39	50.24
Bra019588.1-P	Field mustard	cytosol	44.04	49.87
CDY00194	Canola	cytosol	44.72	48.27
CDX86984	Canola	cytosol	44.5	48.26
Solyc03g071790.1.1	Tomato	cytosol	25.46	47.64
Solyc03g083210.2.1	Tomato	cytosol, nucleus	46.56	46.45
Solyc06g051990.2.1	Tomato	cytosol	49.31	46.04
CDY08119	Canola	cytosol, plastid	44.5	45.97
CDY05915	Canola	cytosol	44.04	45.71
Solyc11g032160.1.1	Tomato	endoplasmic reticulum, extracellular	14.68	45.71
Bra017998.1-P	Field mustard	cytosol	44.27	44.68
KXG31464	Sorghum	cytosol	41.05	44.2
Zm00001d013511_P001	Maize	cytosol	28.44	43.06
Os01t0235200-02	Rice	cytosol	40.6	42.34
Zm00001d053353_P001	Maize	cytosol	25.92	42.32
TraesCS3A01G158200.1	Wheat	cytosol	38.99	37.28
TraesCS3B01G185200.1	Wheat	cytosol	38.99	37.28
TraesCS3D01G165900.1	Wheat	cytosol	38.99	37.28
HORVU3Hr1G057390.1	Barley	cytosol	39.45	35.91
Zm00001d050870_P001	Maize	cytosol	15.6	33.83
Solyc03g083170.2.1	Tomato	cytosol	43.81	24.55
KRH03942	Soybean	cytosol	25.69	9.6

Protein Annotations

EMBL:ACUP02002765	EnsemblPlants:KRH64437	EnsemblPlantsGene:GLYMA_04G235500	EntrezGene:100809510	ncoils:Coil	PANTHER:PTHR33566
PANTHER:PTHR33566:SF1	ProteinID:KRH64437	ProteinID:KRH64437.1	UniParc:UPI0002339F1D	UniProt:I1JYR2	MapMan:12.5.1.14.2

Description

hypothetical protein

Coordinates

chr4:+:50361699..50366933

Molecular Weight (calculated)

48798.6 Da

IEP (calculated)

6.938

GRAVY (calculated)

-0.368

Length

436 amino acids

Sequence
(BLAST)

001: MFQPPTPTGI NKHSLHTNPL SIQGASSALV PVDLNEGTVD AKESVQNGEM LQVQSIFRHS QKLEDDLRML GTKIKQHENN LYHLNSEKNK LDDSILHLQV
101: TIGKSESSSK ATIGDMDNPN PTNDEEVNKQ ILQHEKSAAG ILCQLKIRHG AQASHLTLTK DVVGIVATLG KVEDDNLSRL FSEYLGVETM LAIVCKTYEG
201: VKALEMYDME GCINKSCGLH GLGASIGRAL DGRFLVICLE YLRPYAGNYV VNDAQRKLDF LIPRLPNGEL PSGFLGFAVN MINLDSSNLF CVTPSGYGLR
301: ETLFYNLFSR LQVYKTRAEM IQALPCISEG ALSLDGGMVR SCGVFSLGNR EDVDVRFPRP ERSMRLDNHH IEIERQLKDV KWKKEKIMEE LKREQILLDT
401: TRFNYNKKKA DYLKFLAQSS SDATQAQTAP DRFACR

Best Arabidopsis Sequence Match ( AT3G49250.1 )
(BLAST)

001: MYPTGQQISF QTTPLNVQDP TRMMNLDQSS PVARNETQNG GGIAHAEFAM FNSKRLESDL EAMGNKIKQH EDNLKFLKSQ KNKMDEAIVD LQVHMSKLNS
101: SPTPRSENSD NSLQGEDINA QILRHENSAA GVLSLVETLH GAQASQLMLT KGVVGVVAKL GKVNDENLSQ ILSNYLGTRS MLAVVCRNYE SVTALEAYDN
201: HGNIDINAGL HCLGSSIGRE IGDSFDAICL ENLRPYVGQH IADDLQRRLD LLKPKLPNGE CPPGFLGFAV NMIQIDPAYL LCVTSYGYGL RETLFYNLFS
301: RLQVYKTRAD MISALPCISD GAVSLDGGII RKTGIFNLGN RDEVNVRFAK PTASRTMDNY SEAEKKMKEL KWKKEKTLED IKREQVLREH AVFNFGKKKE
401: EFVRCLAQSS CTNQPMNTPR

Arabidopsis Description

DMS3IDN1 [Source:UniProtKB/TrEMBL;Acc:A0A178VBM0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.