CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 3
cytosol 2
mitochondrion 2

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG98245	nucleus
KRH04373	cytosol
KRH17084	nucleus
KRH28319	cytosol
KRH30203	nucleus
KRH58165	cytosol
KRH72722	nucleus
KRH77167	cytosol

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G26480.1	KRG98245	AT5G58550.1	23512855
AT1G26480.1	KRH17084	AT5G58550.1	23512855
AT1G26480.1	KRH30203	AT5G58550.1	23512855
AT1G26480.1	KRH72722	AT5G58550.1	23512855
AT1G26480.1	KRH04373	AT5G65800.1	23512855
AT1G26480.1	KRH28319	AT5G65800.1	23512855
AT1G26480.1	KRH58165	AT5G65800.1	23512855
AT1G26480.1	KRH77167	AT5G65800.1	23512855

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH75027	Soybean	endoplasmic reticulum	98.46	98.46
VIT_01s0011g00620.t01	Wine grape	cytosol	92.28	90.19
KRH50527	Soybean	cytosol	88.8	88.8
KRG89486	Soybean	cytosol	88.8	88.46
Solyc01g010360.2.1	Tomato	cytosol	88.03	83.21
Bra024713.1-P	Field mustard	cytosol	84.17	81.65
Bra016273.1-P	Field mustard	cytosol	84.94	81.48
AT1G26480.1	Thale cress	cytosol	83.78	80.97
CDY16161	Canola	cytosol	83.78	80.67
CDX85023	Canola	cytosol	83.78	80.37
Solyc05g012420.2.1	Tomato	unclear	88.03	80.0
PGSC0003DMT400072941	Potato	cytosol	87.26	79.3
CDY40251	Canola	cytosol	74.9	79.18
KRH22287	Soybean	nucleus, plasma membrane	75.29	74.71
KRH27042	Soybean	nucleus	75.29	74.71
KRH42856	Soybean	nucleus	74.52	74.52
KRH58958	Soybean	endoplasmic reticulum	74.52	74.52
CDY20560	Canola	cytosol	74.52	72.28
KRH21981	Soybean	cytosol	71.81	70.72
KRH27339	Soybean	cytosol	71.81	70.19
KRH01783	Soybean	nucleus	69.5	70.04
KRH72385	Soybean	endoplasmic reticulum, nucleus	68.73	67.94
KRH46909	Soybean	cytosol	67.95	67.69
CDY61631	Canola	cytosol	84.94	67.07
CDY24128	Canola	cytosol	84.56	66.77
KRG89721	Soybean	cytosol	17.76	65.71
KRH52909	Soybean	endoplasmic reticulum	62.55	64.8
KRH62193	Soybean	endoplasmic reticulum	62.16	64.4
Bra012455.1-P	Field mustard	cytosol	74.9	63.61
KRH53025	Soybean	cytosol	68.34	63.44
KRH62315	Soybean	endoplasmic reticulum, mitochondrion, nucleus, plasma membrane	69.88	62.85
KRH16772	Soybean	cytosol	69.11	57.01
KRH05108	Soybean	cytosol	28.19	45.91

Protein Annotations

Gene3D:1.20.190.20	EntrezGene:100529129	InterPro:14-3-3	InterPro:14-3-3_CS	InterPro:14-3-3_dom_sf	InterPro:14-3-3_domain
MapMan:35.1	EMBL:ACUP02000818	ncoils:Coil	UniProt:E9KNA5	EnsemblPlantsGene:GLYMA_02G115900	GO:GO:0003674
GO:GO:0005488	GO:GO:0005515	GO:GO:0019904	EMBL:HM004358	InterPro:IPR036815	ProteinID:KRH70878.1
EnsemblPlants:KRH70879	ProteinID:KRH70879	ProteinID:KRH70879.1	PFAM:PF00244	PIRSF:PIRSF000868	PRINTS:PR00305
ScanProsite:PS00796	ScanProsite:PS00797	PANTHER:PTHR18860	PANTHER:PTHR18860:SF12	SMART:SM00101	SUPFAM:SSF48445
UniParc:UPI00018C707F	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr2:+:11280858..11285984

Molecular Weight (calculated)

29499.7 Da

IEP (calculated)

4.539

GRAVY (calculated)

-0.580

Length

259 amino acids

Sequence
(BLAST)

001: MSVEKERETQ VYLAKLAEQA ERYEEMVECM KKVAKLDLDL TVEERNLLSV GYKNVIGARR ASWRIMSSIE QKEESKGNEH NVKLIKSYCQ KVEEELSKIC
101: GDILTIIDQH LIPSSGSAEA SVFYYKMKGD YFRYLAEFKT DQERKEAAEQ SLKGYEAASA TANTDLPSTH PIRLGLALNF SVFYYEIMNS PERACHLAKQ
201: AFDEAIAELD TLSEESYKDS TLIMQLLRDN LTLWTSDLPE DGGEDNIKAE EAKPSEPEH

Best Arabidopsis Sequence Match ( AT1G26480.1 )
(BLAST)

001: MSSSGSDKER ETFVYMAKLS EQAERYDEMV ETMKKVARVN SELTVEERNL LSVGYKNVIG ARRASWRIMS SIEQKEESKG NESNVKQIKG YRQKVEDELA
101: NICQDILTII DQHLIPHATS GEATVFYYKM KGDYYRYLAE FKTEQERKEA AEQSLKGYEA ATQAASTELP STHPIRLGLA LNFSVFYYEI MNSPERACHL
201: AKQAFDEAIA ELDTLSEESY KDSTLIMQLL RDNLTLWTSD LPEDGGEDNI KTEESKQEQA KPADATEN

Arabidopsis Description

GRF12GRF12 [Source:UniProtKB/TrEMBL;Acc:A0A178WJF9]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.