CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 3
cytosol 2
mitochondrion 2

Predictors

GFP

MS/MS

Papers

endoplasmic reticulum: 23041469
endoplasmic reticulum: 27224218

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG98245	nucleus
KRH04373	cytosol
KRH17084	nucleus
KRH28319	cytosol
KRH30203	nucleus
KRH58165	cytosol
KRH72722	nucleus
KRH77167	cytosol

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G26480.1	KRG98245	AT5G58550.1	23512855
AT1G26480.1	KRH17084	AT5G58550.1	23512855
AT1G26480.1	KRH30203	AT5G58550.1	23512855
AT1G26480.1	KRH72722	AT5G58550.1	23512855
AT1G26480.1	KRH04373	AT5G65800.1	23512855
AT1G26480.1	KRH28319	AT5G65800.1	23512855
AT1G26480.1	KRH58165	AT5G65800.1	23512855
AT1G26480.1	KRH77167	AT5G65800.1	23512855

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH70879	Soybean	endoplasmic reticulum	98.46	98.46
VIT_01s0011g00620.t01	Wine grape	cytosol	91.51	89.43
KRH50527	Soybean	cytosol	88.8	88.8
KRG89486	Soybean	cytosol	88.42	88.08
Solyc01g010360.2.1	Tomato	cytosol	87.26	82.48
Bra024713.1-P	Field mustard	cytosol	83.78	81.27
Bra016273.1-P	Field mustard	cytosol	84.17	80.74
CDY16161	Canola	cytosol	83.78	80.67
AT1G26480.1	Thale cress	cytosol	83.4	80.6
CDX85023	Canola	cytosol	83.4	80.0
Solyc05g012420.2.1	Tomato	unclear	87.26	79.3
CDY40251	Canola	cytosol	74.52	78.78
PGSC0003DMT400072941	Potato	cytosol	86.49	78.6
KRH22287	Soybean	nucleus, plasma membrane	76.45	75.86
KRH27042	Soybean	nucleus	76.45	75.86
KRH42856	Soybean	nucleus	74.52	74.52
KRH58958	Soybean	endoplasmic reticulum	74.52	74.52
CDY20560	Canola	cytosol	74.13	71.91
KRH01783	Soybean	nucleus	70.27	70.82
KRH27339	Soybean	cytosol	72.2	70.57
KRH21981	Soybean	cytosol	71.43	70.34
KRH46909	Soybean	cytosol	68.34	68.08
KRH72385	Soybean	endoplasmic reticulum, nucleus	68.73	67.94
CDY61631	Canola	cytosol	84.17	66.46
CDY24128	Canola	cytosol	83.78	66.16
KRG89721	Soybean	cytosol	17.76	65.71
KRH52909	Soybean	endoplasmic reticulum	62.55	64.8
KRH62193	Soybean	endoplasmic reticulum	62.16	64.4
KRH53025	Soybean	cytosol	68.34	63.44
Bra012455.1-P	Field mustard	cytosol	74.52	63.28
KRH62315	Soybean	endoplasmic reticulum, mitochondrion, nucleus, plasma membrane	69.5	62.5
KRH16772	Soybean	cytosol	69.11	57.01
KRH05108	Soybean	cytosol	28.57	46.54

Protein Annotations

Gene3D:1.20.190.20	EntrezGene:100529130	InterPro:14-3-3	InterPro:14-3-3_CS	InterPro:14-3-3_dom_sf	InterPro:14-3-3_domain
MapMan:35.1	EMBL:ACUP02000107	ncoils:Coil	UniProt:E9KNA7	EnsemblPlantsGene:GLYMA_01G058000	GO:GO:0003674
GO:GO:0005488	GO:GO:0005515	GO:GO:0019904	EMBL:HM004360	InterPro:IPR036815	EnsemblPlants:KRH75027
ProteinID:KRH75027	ProteinID:KRH75027.1	PFAM:PF00244	PIRSF:PIRSF000868	PRINTS:PR00305	ScanProsite:PS00796
ScanProsite:PS00797	PANTHER:PTHR18860	PANTHER:PTHR18860:SF12	SMART:SM00101	SUPFAM:SSF48445	UniParc:UPI0001F72C45

Description

hypothetical protein

Coordinates

chr1:+:7642485..7646277

Molecular Weight (calculated)

29488.7 Da

IEP (calculated)

4.539

GRAVY (calculated)

-0.580

Length

259 amino acids

Sequence
(BLAST)

001: MSAEKERETQ VYLAKLAEQA ERYEEMVECM KKVAKLDLDL TVEERNLLSV GYKNVIGARR ASWRIMSSIE QKEESKGNEH NVKLIKSYCQ KVEEELSKIC
101: GDILTIIDQH LIPSSASAEA SVFYYKMKGD YFRYLAEFKT DQERKEAAEQ SLKGYEAASA TANTDLPSTH PIRLGLALNF SVFYYEIMNS PERACHLAKQ
201: AFDEAIAELD TLSEESYKDS TLIMQLLRDN LTLWTSDLPE DGGEDSIKAE ETKPSEPEH

Best Arabidopsis Sequence Match ( AT1G26480.1 )
(BLAST)

001: MSSSGSDKER ETFVYMAKLS EQAERYDEMV ETMKKVARVN SELTVEERNL LSVGYKNVIG ARRASWRIMS SIEQKEESKG NESNVKQIKG YRQKVEDELA
101: NICQDILTII DQHLIPHATS GEATVFYYKM KGDYYRYLAE FKTEQERKEA AEQSLKGYEA ATQAASTELP STHPIRLGLA LNFSVFYYEI MNSPERACHL
201: AKQAFDEAIA ELDTLSEESY KDSTLIMQLL RDNLTLWTSD LPEDGGEDNI KTEESKQEQA KPADATEN

Arabidopsis Description

GRF12GRF12 [Source:UniProtKB/TrEMBL;Acc:A0A178WJF9]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.